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Effect of intra-erythrocytic magnesium ions on invasion by Plasmodium falciparum

Published online by Cambridge University Press:  06 April 2009

S. J. Field ,
K. Rangachari ,
A. R. Dluzewski ,
R. J. M. Wilson  and
W. B. Gratzer
S. J. Field
Affiliation:
Medical Research Council Muscle and Cell Motility Unit, King's College, 26–29 Drury Lane, London WC2B 5RL
K. Rangachari
Affiliation:
Medical Research Council Muscle and Cell Motility Unit, King's College, 26–29 Drury Lane, London WC2B 5RL National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
A. R. Dluzewski
Affiliation:
Medical Research Council Muscle and Cell Motility Unit, King's College, 26–29 Drury Lane, London WC2B 5RL
R. J. M. Wilson
Affiliation:
National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
W. B. Gratzer
Affiliation:
Medical Research Council Muscle and Cell Motility Unit, King's College, 26–29 Drury Lane, London WC2B 5RL
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Extract

Exclusion of magnesium ions from resealed ghosts or their extraction from intact human red cells by means of an ionophore results in a reversible drop in susceptibility to invasion by Plasmodium falciparum merozoites in vitro. Resealed ghosts, containing magnesium-ATP and diluted cytosol, are invaded with high efficiency only when the original hypotonic lysis is carried Out in the presence of magnesium ions. This effect is not related to the loss of membrane-associated constituents when magnesium ions are absent. Ghosts containing calcium ions, together with the protective agent, flunarizine, were essentially resistant to invasion; this effect is again at least partially reversible. A possible explanation of these phenomena is that entry of the merozoite may be inhibited by breakdown of the host cell phospholipid asymmetry, with the appearance of aminophospholipids at the outer cell surface.

Keywords

Plasmodiurn falciparummerozoitemagnesiumphospholipid asymmetryred blood cells.
Type
Research Article
Information
Parasitology , Volume 105 , Issue 1 , August 1992 , pp. 15 - 19
Copyright
Copyright © Cambridge University Press 1992

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