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The effect of Blastocrithidia triatomae (Trypanosomatidae) on the midgut of the reduviid bug Triatoma infestans

Published online by Cambridge University Press:  06 April 2009

C. Jensen ,
G. A. Schaub  and
D. H. Molyneux
C. Jensen
Affiliation:
Institut für Biologie I (Zoologie), Albert-Ludwigs-Universität, Albertstrasse 21a, D-7800 Freiburg, Federal Republic of Germany
G. A. Schaub
Affiliation:
Institut für Biologie I (Zoologie), Albert-Ludwigs-Universität, Albertstrasse 21a, D-7800 Freiburg, Federal Republic of Germany
D. H. Molyneux
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT, England
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Summary

The pathogenic flagellate Blastocrithidia triatomae disrupts the digestion of Triatoma infestans; the midgut ultrastructure of bugs infected with the flagellate and of uninfected bugs is compared. Third or fourth instar larvae were dissected either unfed or 1 week after feeding.In all uninfected bugs extracellular membrane layers (e.m.l.) covered the apical microvillar border of the epithelial cells. Some midgut regions of bugs infected with B. triatomae appeared normal but often adjacent cells showed pathological effects. In affected cells the e.m.l. and the microvilli and finally the cells themselves were reduced or destroyed. Correlated with these observations of pathogenicity the method of attachment of parasites changed. When the e.m.l. were present only rarely were flagella found, but on extracellular membrane-free cells B. triatomae attached by flagellar enlargement to the microvillar border or, if this was reduced, to the apical host cell membrane. No hemidesmosome-like plaques were found at the attachment site. Although some flagella were inserted into the apical region of the cells no intracellular flagellates were observed.

Keywords

Blastocrithidia triatomaeTriatoma infestansmidgut ultrastructurepathogenicity
Type
Research Article
Information
Parasitology , Volume 100 , Issue 1 , February 1990 , pp. 1 - 9
Copyright
Copyright © Cambridge University Press 1990

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