Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-26T16:12:47.989Z Has data issue: false hasContentIssue false
  • English
  • Français

DNA comparisons of Trypanosoma evansi (Indonesia) and Trypanosoma brucei spp.

Published online by Cambridge University Press:  06 April 2009

W. T. Artama ,
M. W. Agey  and
J. E. Donelson
W. T. Artama
Affiliation:
IUC Biotechnology, University of Gadjah Mada, Jl. Teknika Utara, BarekYogyakarta, Indonesia Howard Hughes Medical Institute, Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
M. W. Agey
Affiliation:
Howard Hughes Medical Institute, Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
J. E. Donelson
Affiliation:
Howard Hughes Medical Institute, Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242, USA
Get access Rights & Permissions [Opens in a new window]

Summary

Two clones from separate isolates of Trypanosoma evansi in Indonesia were found by polymerase chain reaction (PCR) analyses to contain 3 different repeated nuclear DNA sequences of Trypanosoma brucei spp: the consensus sequence for a highly repetitive 177 base pairs and the gene repeats encoding procyclin and the spliced leader. In addition, the 994 bp minicircle sequence of one of the clones was determined, and PCR amplification primers specific for minicircles of T. evansi were identified that do not amplify minicircle sequences in the T. brucei spp. clones tested.

Keywords

Trypanosoma evansiminicircle DNApolymerase chain reactiongene repeats
Type
Research Article
Information
Parasitology , Volume 104 , Issue 1 , February 1992 , pp. 67 - 74
Copyright
Copyright © Cambridge University Press 1992

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Barrois, M., Riou, G. & Galibert, F. (1981). Complete nucleotide sequence of minicircle kinetoplast DNA from Trypanosoma equiperdum. Proceedings of the National Academy of Sciences, USA 78, 3323–7.CrossRefGoogle ScholarPubMed
Benne, R. (1989). RNA editing in trypanosome mitochondria. Biochimica et Biophysica Acta 1007, 131–9.CrossRefGoogle ScholarPubMed
Blum, B., Bakalara, N. & Simpson, L. (1990). A model for RNA editing in kinetoplastid mitochondria; ‘guide’ RNA molecules transcribed from maxicircle DNA provide the edited information. Cell 60, 189–98.CrossRefGoogle Scholar
Borst, P., Fase-Fowler, F. & Gibson, W. C. (1987). Kinetoplast DNA of Trypanosoma evansi. Molecular and Biochemical Parasitology 23, 31–8.CrossRefGoogle ScholarPubMed
Borst, P., Fairlamb, A. H., Fase-Fowler, F., Hoeijmakers, J. H. & Weislogel, P. O. (1976). The structure of kinetoplast DNA. In The Genetic Function of Mitochondrial DNA (ed. Saccone, C. & Kroon, A. M.), pp. 59–69: Amsterdam, North-Holland.Google Scholar
Chen, K. K. & Donelson, J. E. (1980). Sequences of two kinetoplast DNA minicircles of Trypanosoma brucei. Proceedings of the National Academy of Sciences, USA 77, 2445–9.CrossRefGoogle Scholar
Clayton, C. E., Fueri, J. P., Itzhaki, J. E., Bellofatto, V., Sherman, D. R., Wisdom, G. S., Vijayasarathy, S. & Mowalt, M. R. (1990). Transcription of the procyclic acidic repetitive protein genes of Trypanosoma brucei. Molecular and Cellular Biology 10, 3036–47.Google ScholarPubMed
Donelson, J. E., Majiwa, P. A. O., Williams, R. O. (1979). Kinetoplast DNA minicircles of Trypanosoma brucei share regions of sequence homology. Plasmid 2, 572–88.CrossRefGoogle ScholarPubMed
Donelson, J. E., Majiwa, P. A. O. & Williams, R. O. (1979). Kinetoplast DNA minicircles of Trypanosoma brucei share regions of sequence homology. Plasmid 2CrossRefGoogle ScholarPubMed
Dorfman, D. M. & Donelson, J. E. (1984). Characterization of the 1.35 kb DNA repeat unit containing the conserved 35 nucleotides at the 5 termini of variant surface glycoprotein mRNAs in Trypanosoma brucei. Nucleic Acid Research 12, 4907–20.CrossRefGoogle ScholarPubMed
Frasch, A. C. C., Hajduk, S. L., Heoijmakers, J. H. J., Borst, P., Brunel, F. & Davison, J. (1980). The kinetoplast DNA of Trypanosoma equiperdum. Biochimica et Biophysica Acta 607, 397–410.CrossRefGoogle ScholarPubMed
Gibson, W. C., Wilson, A. J. & Moloo, S. K. (1983). Characterization of Trypanosoma (Trypanozoon) evansi from camels in Kenya using isoenzyme electrophoresis. Research in Veterinary Science 34, 114–18.CrossRefGoogle ScholarPubMed
Hattori, M. & Sakaki, Y. (1986). Dideoxy sequencing method using denatured plasmid templates. Analytical Biochemistry 152, 232–8.CrossRefGoogle ScholarPubMed
Jasmer, D. P. & Stuart, K. (1986 a). Conservation of kinetoplastid minicircle characteristics without nucleotide sequence conservation. Molecular and Biochemical Parasitology 18, 257–69.CrossRefGoogle ScholarPubMed
Jasmer, D. P. & Stuart, K. (1986 b). Sequence organization in African trypanosome minicircles is defined by 18 bp inverted repeats. Molecular and Biochemical Parasitology 18, 321–31.CrossRefGoogle Scholar
Kanthack, A. A., Durham, H. E. & Blandford, W. F. H. (1898). On nagana or tsetse-fly disease. Proceedings of the Royal Society 64, 100.Google Scholar
Lanham, S. M. & Godfrey, D. G. (1970). Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Experimental Parasitology 28, 521–34.CrossRefGoogle ScholarPubMed
Laurent, M., Van Assel, S. & Steinert, M. (1971). A unique macromolecular structure of considerable size and mechanical resistance. Biochemical and Biophysical Research Communications 43, 278–84.CrossRefGoogle ScholarPubMed
Lenardo, M. J., Rice-Ficht, A. C., Kelly, G., Esser, K. & Donelson, J. E. (1984). Characterization of the genes specifying two metacyclic variant surface antigen types in Trypanosoma brucei rhodesiense. Proceedings of the National Academy of Sciences, USA 81, 6642–6.CrossRefGoogle Scholar
Marini, J. C., Levene, S. D., Crothers, D. M. & Englund, P. T. (1982). Bent helical structure in kinetoplast DNA. Proceedings of the National Academy of Sciences, USA 79, 7664–8.CrossRefGoogle ScholarPubMed
Masiga, D. K. & Gibson, W. C. (1990). Specific probes for Trypanosoma (Trypanozoon) evansi based on kinetoplast DNA minicircles. Molecular and Biochemical Parasitology 40, 279–84.CrossRefGoogle ScholarPubMed
Mohan, R. N. (1968). Diseases and parasites of buffaloes. Part III. Parasitic and miscellaneous diseases. Veterinary Bulletin 38, 735.Google Scholar
Ntambi, J. M. & Englund, P. T. (1985). A gap at the unique location in newly replicated kinetoplast DNA minicircles from Trypanosoma equiperdum. Journal of Biological Chemistry 260, 5574–9.CrossRefGoogle ScholarPubMed
Ou, Y. C. & Baltz, T. (1991). Kinetoplast DNA analysis of four Trypanosoma evansi strains. Molecular and Biochemical Parasitology 46, 97–102.CrossRefGoogle ScholarPubMed
Pollard, V. W. & Hajduk, S. L. (1991). Trypanosoma equiperdum minicircles encode three distinct primary transcripts which exhibit guide RNA characteristics. Molecular and Cellular Biology 11, 1668–75.Google ScholarPubMed
Pollard, V. W., Rohrer, S. P., Michelotti, E. F., Hancock, K. & Hajduk, S. L. (1990). Organization of minicircle genes for guide RNAs in Trypanosoma brucei. Cell 63, 783–90.CrossRefGoogle ScholarPubMed
Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, New York.Google Scholar
Silva, L. H. P. & Nussenzweig, V. (1953). Sobre uma cepa de Trypanosoma cruzi altamente virulenta para o camundongo branco. (On a strain of Trypanosoma cruzi highly virulent for white mice.) Folia Clinical Biologie 20, 197–207.Google Scholar
Simpson, L. (1986). Kinetoplast DNA in trypanosomatid flagellates. International Review of Cytology 99, 119–79.CrossRefGoogle Scholar
Sloof, P., Menke, H. H., Caspers, M. P. & Borst, P. (1983). Size fractionation of Trypanosoma brucei DNA: localization of the 177-bp repeat satellite DNA and a variant surface glycoprotein gene in a minichromosomal DNA fraction. Nucleic Acids Research 11, 3889–901.CrossRefGoogle Scholar
Songa, E. B., Paindavoine, P., Wittouck, E., Viseshakul, N., Muldermans, S., Steinert, M. & Hamers, R. (1990). Evidence for kinetoplast and nuclear DNA homogeneity in Trypanosoma evansi isolates. Molecular and Biochemical Parasitology 43, 167–80.CrossRefGoogle ScholarPubMed
Stephen, L. E. (1986). Trypanosomiasis, a Veterinary Perspective. Oxford: Pergamon Press.Google Scholar
Sturm, N. R. & Simpson, L. (1990). Kinetoplast DNA minicircles encode guide RNAs for editing cytochrome oxidase subunit III mRNA. Cell 61, 879–84.CrossRefGoogle ScholarPubMed
World Health Organization (1978). Proposals for the nomenclature of salivarian trypanosomes and for the maintenance of reference collections. Bulletin of the World Health Organization 56, 467–80.Google Scholar