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DNA barcoding of Cryptosporidium

Published online by Cambridge University Press:  08 November 2017

JAN ŠLAPETA Open the ORCID record for JAN ŠLAPETA [Opens in a new window]
JAN ŠLAPETA*
Affiliation:
Sydney School of Veterinary Science, Faculty of Science, University of Sydney, Sydney, New South Wales, Australia
*
*Corresponding author: Sydney School of Veterinary Science, Faculty of Science, University of Sydney, Sydney, New South Wales, Australia. E-mail: [email protected]
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Summary

Cryptosporidium spp. (Apicomplexa) causing cryptosporidiosis are of medical and veterinary significance. The genus Cryptosporidium has benefited from the application of what is considered a DNA-barcoding approach, even before the term ‘DNA barcoding’ was formally coined. Here, the objective to define the DNA barcode diversity of Cryptosporidium infecting mammals is reviewed and considered to be accomplished. Within the Cryptosporidium literature, the distinction between DNA barcoding and DNA taxonomy is indistinct. DNA barcoding and DNA taxonomy are examined using the latest additions to the growing spectrum of named Cryptosporidium species and within-species and between-species identity is revisited. Ease and availability of whole-genome DNA sequencing of the relatively small Cryptosporidium genome offer an initial perspective on the intra-host diversity. The opportunity emerges to apply a metagenomic approach to purified field/clinical Cryptosporidum isolates. The outstanding question remains a reliable definition of Cryptosporidium phenotype. The complementary experimental infections and metagenome approach will need to be applied simultaneously to address Cryptosporidium phenotype with carefully chosen clinical evaluations enabling identification of virulence factors.

Keywords

DNA barcodingDNA taxonomysystematicsCryptosporidiumApicomplexapublic health
Type
Special Issue Review
Information
Parasitology , Volume 145 , Special Issue 5: Advances and challenges in barcoding of microbes, parasites, and their vectors and reservoirs , April 2018 , pp. 574 - 584
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Copyright
Copyright © Cambridge University Press 2017 

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