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Dipetalonema viteae in hamsters: effect of antiserum or immunization with parasite extracts on production of microfilariae

Published online by Cambridge University Press:  06 April 2009

A. Haque ,
M. N. Lefebvre ,
B. M. Ogilvie  and
A. Capron
A. Haque
Affiliation:
Institut Pasteur, 59012 Lille Cedex, France
M. N. Lefebvre
Affiliation:
Institut Pasteur, 59012 Lille Cedex, France
B. M. Ogilvie
Affiliation:
National Institute for Medical Research, Mill Hill, London NW7 1AA
A. Capron
Affiliation:
Institut Pasteur, 59012 Lille Cedex, France
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Summary

After hamsters have been infected with Dipetalonema viteae for about 4 months, microfilariae disappear from the circulation but the adult worms remain alive and active. Such hamsters are said to harbour a ‘latent’ infection. Additional adult worms implanted into such hamsters produce no microfilariae, but adult worms recovered from hamsters with latent infections resume microfilariae production when surgically transplanted into new hosts. In vitro, worms from hamsters with latent infections renewed microfilariae production in the presence of serum from uninfected but not infected hamsters. Serum from infected animals had a marked suppressive effect on the microfilariae production of adult worms implanted into passively immunized hamsters. This suggests that microfilarial release is regulated by a serum factor. Microfilarial production in in vitro and in vivo experiments was more strongly inhibited by serum taken from hamsters with latent infections and hamsters infected for only 30 days (pre-patent) than by serum taken 90 days after infection (whilst microfilariae were in the circulation of the serum donors). A cytotoxic effect of sera from infected hamsters on the microfilariae could not be demonstrated.

In animals immunized by extracts of adult worms or microfilariae, microfilaraemia was considerably reduced. In contrast, animals immunized repeatedly with the extract from small numbers of microfilariae during the pre-patent phase of an infection had an enhanced microfilaraemia. Adult worm numbers were not affected by immunization except in animals given extract from a single female worm which harboured fewer worms than controls.

Type
Research Article
Information
Parasitology , Volume 76 , Issue 1 , February 1978 , pp. 61 - 75
Copyright
Copyright © Cambridge University Press 1978

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References

REFERENCES

Biguet, J., Capron, A., Tran Van Ky, P. & Rose, F. (1965). Présence do substance de type C dans les antigènes vermineux et de protéine anti-C au cours des helminthiases humaines et expérimentales. Revue d'immunologie, Paris 29, 233–40.Google Scholar
Butterworth, A. E., Sturrock, B. F., Houba, V. & Rees, P. H. (1974). Antibody-dependent cell-mediated damage to schistosomula in vitro. Nature, London 252, 503–5.CrossRefGoogle ScholarPubMed
Capron, A., Biguet, J., Vernes, A. & Afchain, D. (1968). Structure antigénique des helminthes. Aspects immunologiques des relations hôte-parasite. Pathologie-Biologie 16, 121–38.Google Scholar
Capron, A., Gentilini, M. & Vernes, A. (1968). Le diagnostic immunologique de filarioses. Possibilitiés nouvelles offertes par immuno-électrophorèse. Pathologie-Biologie 16, 1039–45.Google Scholar
Danaraj, T.J., Pacheco, G., Shanmugaratnum, K. & Beaver, P. C. (1966). The etiology and pathology of eosinophilic lung (tropical eosinophilia). American Journal of Tropical Medicine and Hygiene 15, 183–9.CrossRefGoogle ScholarPubMed
Denham, D. A., Ponnudurai, T., Nelson, G. S., Rogers, R. & Guy, F. (1972). Studies with Brugia pahangi. II. The effect of repeated infection on parasite levels in cats. International Journal for Parasitology 2, 401–7.CrossRefGoogle ScholarPubMed
Hanks, J. H. & Wallace, R. E. (1949). Relation of oxygen and temperature in the preservation of tissues by refrigeration. proceedings of the Society for Experimental Biology and Medicine 71, 196–200.CrossRefGoogle ScholarPubMed
Jordan, P. (1955). Notes on elephantiasis and hydrocoele due to Wuchereria bancrofti. Journal of Tropical Medicine and Hygiene 15, 183–9.Google Scholar
Knott, J. (1939). A method for making microfilarial surveys on day blood. Transactions of the Royal Society of Tropical Medicine and Hygiene 33, 191–6.CrossRefGoogle Scholar
Manson, P. (1899). Tropical Diseases. A manual of the Diseases of Warm Climates. London: Cassell and Company, Ltd.Google Scholar
Neilson, J. T. M. & Forrester, D. J. (1975). The dynamics of primary, secondary and tertiary infections of Dipetalonema viteae in hamsters. Experimental Parasitology 37, 367–72.CrossRefGoogle Scholar
Obeck, D. K. (1973). Blood microfilariae: new and existing techniques for isolation. Journal of Parasitology 59, 220–9.CrossRefGoogle ScholarPubMed
Pacheco, G. (1970). Infection and superinfection of jirds (Meriones unguiculatus) and hamsters (Mesocricetus auratus) with Dipetalonema viteae. Journal of Parasitology 56, 255–6.Google Scholar
Ponnudurai, T., Denham, D. A., Nelson, G. S. & Rogers, R. (1974). Studies with Brugia pahangi 4. Antibodies against adult and microfilarial stages. Journal of Helminthology 48, 107–11.CrossRefGoogle ScholarPubMed
Ramakrishnan, S. P., Singh, D. & Krishnaswami, A. K. (1962). Evidence of acquired immunity against microfilaria of Litomosoides carinii in albino rats with mite-induced infection. Indian Journal of Malariology 16, 263–8.Google ScholarPubMed
Rilley, V. (1960). Adaptation of orbital bleeding technique to rapid serial blood studies. Proceedings of the Society for Experimental Biology and Medicine 104, 751–4.CrossRefGoogle Scholar
Subramanyam, D., Rao, Y. V. B. G., Metita, K. & Nelson, D. S. (1976). Serum-dependent adhesion and cytotoxicity of cells to Litomosoides carinii microfilariae. Nature, London 260, 529–30.CrossRefGoogle Scholar
Weiss, N. (1970). Parasitologische und immunobiologische UnterSuchungen über die durch Dipetalonema viteae erzeugte Nagetierfilariose. Acta Tropica 27, 219‐59.Google Scholar
Wong, M. M. (1964 a). Studies on microfilaraemia in dogs. I. A search for the mechanisms that stabilize the level of microfilaraemia. American Journal of Tropical Medicine and Hygiene 13, 57–65.CrossRefGoogle Scholar
Wong, M. M. (1964 b). Studies on microfilaraemia in dogs. II. Levels of microfilaraemia in relation to immunologic responses of the host. American Journal of Tropical Medicine and Hygiene 13, 66–77.CrossRefGoogle Scholar
Worms, M. J. (1972). The course of microfilaraemia in primary infections of Dirofilaria immitis and D. repene in dogs. Comptes Rendus 1er Multicolloque Européen de Rennes, 324–6.Google Scholar