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Differentiation of Schistosoma mansoni from S. rodhaini using cloned DNA probes

Published online by Cambridge University Press:  06 April 2009

Tina K. Walker ,
A. J. G. Simpson  and
D. Rollinson
Tina K. Walker
Affiliation:
Department of Zoology, British Museum (Natural History), Cromwell Road, South Kensington, London SW7 5BD
A. J. G. Simpson
Affiliation:
Division of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA
D. Rollinson
Affiliation:
Department of Zoology, British Museum (Natural History), Cromwell Road, South Kensington, London SW7 5BD
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Summary

The ribosomal RNA (rRNA) gene units of Schistosoma mansoni and S. rodhaini, of the lateral spined egg group, have been studied. The schistosome rRNA gene unit consists of a regular interspersion of the two genes encoding the large and small rRNA units with two spacers. The large spacer is not transcribed while the small spacer is part of the transcription unit. Variation in the rRNA gene unit between the two species is demonstrated to take the form of loss or gain of restriction sites within the non-transcribed and transcribed spacers. This variation has been demonstrated to enable the differentiation of S. mansoni from S. rodhaini by Southern hybridization analysis. In addition, a DNA clone representing a female specific, tandemly repeated sequence, is demonstrated to enable differentiation of S. mansoni and S. rodhaini using dot blot hybridization analysis.

Keywords

Schistosoma sppdifferentiationcloned DNA probes
Type
Research Article
Information
Parasitology , Volume 98 , Issue 1 , February 1989 , pp. 75 - 80
Copyright
Copyright © Cambridge University Press 1989

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