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Differentiation between human and animal strains of Cryptosporidium parvum using isoenzyme typing

Published online by Cambridge University Press:  06 April 2009

F. M. Awad-El-Kariem ,
H. A. Robinson ,
D. A. Dyson ,
D. Evans ,
S. Wright ,
M. T. Fox  and
V. Mcdonald
F. M. Awad-El-Kariem
Affiliation:
Departments of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
H. A. Robinson
Affiliation:
Departments of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
D. A. Dyson
Affiliation:
Veterinary Investigation Centre, Ministry of Agriculture, Fisheries and Food, Calthwaite, Penrith, Cumbria CA11 9RR
D. Evans
Affiliation:
Clinical Sciences and Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
S. Wright
Affiliation:
Moredun Research Institute, 408 Gilmerton Road, Edinburgh EH17 7JH
M. T. Fox
Affiliation:
Department of Pathology, Royal Veterinary College, Royal College Street, London NW1
V. Mcdonald
Affiliation:
Departments of Medical Parasitology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
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Summary

Isoenzyme typing was used to study a number of oocyst isolates of Cryptosporidium parvum from different geographical locations and of human or animal origin. All isolates showed identical enzyme motility when glucose phosphate isomerase (GPI; 23 isolates tested) or lactate dehydrogenases (LDH; 20 isolates tested) was assayed. However, two isoenzyme forms were observed with phosphoglucomutase (PGM; 9 animal isolates showed one form, while 8/9 human isolates showed a second form) and hexokinase (HK; 4 human isolates showed one form and 6 animal isolates showed a second form). Thus, PGM and HK each exhibit 2 isoenzymes corresponding to 2 parasite populations associated with separate hosts. The data from this study, plus supportive evidence obtained by different methods and by independent researchers, lend support to the hypothesis that separate cycles of transmission of C. parvum may exist within human and animal hosts.

Keywords

Cryptosporidium parvumisoenzyme electrophoresiszoonotic transmissionhost specificity
Type
Research Article
Information
Parasitology , Volume 110 , Issue 2 , February 1995 , pp. 129 - 132
Copyright
Copyright © Cambridge University Press 1995

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