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Differential infectivity and immunopathology in murine experimental infections by two natural clones belonging to the Trypanosoma cruzi I lineage

Published online by Cambridge University Press:  09 March 2005

E. GARZON ,
F. GENNA ,
M. F. BOSSENO ,
J. SIMONY-LA FONTAINE ,
M. RADAL ,
D. SERENO ,
F. MATHIEU-DAUDE ,
A. OUAISSI  and
S. F. BRENIÈRE
E. GARZON
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
F. GENNA
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
M. F. BOSSENO
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
J. SIMONY-LA FONTAINE
Affiliation:
Département d'Anatomopathologie, CRLC Val d'Aurelle-Paul Lamarque, Parc Euromédecine, 34298, Montpellier cédex 5, France
M. RADAL
Affiliation:
Département d'Anatomopathologie, CRLC Val d'Aurelle-Paul Lamarque, Parc Euromédecine, 34298, Montpellier cédex 5, France
D. SERENO
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
F. MATHIEU-DAUDE
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
A. OUAISSI
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
S. F. BRENIÈRE
Affiliation:
Institut de Recherche pour le Développement, IRD, UR 008 “Pathogénie et Epidémiologie des Trypanosomatidés”, 911 Av. Agropolis, BP 64501, 34394 Montpellier Cedex 5, France
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Abstract

Immunopathology of Chagas' disease in Balb/c mice infected with 2 Trypanosoma cruzi clones, belonging to the T. cruzi I lineage and presenting different in vitro virulence (P/209 cl1>SO34 cl4) was compared. In the acute phase, evading mechanisms such as parasite-induced lymphocyte polyclonal activation and T cell immunosuppression were higher in mice infected with the clone giving a higher parasitaemia (P/209 cl1). A similar increase of non-specific isotypes was observed in both infections with IgG2a prevalence. Interestingly, CD8+ cell hypercellularity and lymphocyte immunosuppression were observed during the chronic phase (245 days post-infection) in mice infected by the most virulent clone. In the same way, the parasite-specific antibody response was more intense in P/209 cl1-infected mice over the acute phase. During the chronic phase this response remarkably dropped down in SO34 cl4-infected mice exclusively. Finally, P/209 cl1-infected mice presented a more severe inflammation and tissue damage in heart and quadriceps than SO34 cl4-infected mice. This comparative study showed differences between the two clones: a higher virulence in vivo being clearly associated with a greater ability to induce evasion mechanisms and severe tissue damage.

Keywords

Trypanosoma cruzigenotypeimmunopathologyvirulencemouse
Type
Research Article
Information
Parasitology , Volume 131 , Issue 1 , July 2005 , pp. 109 - 119
Copyright
© 2005 Cambridge University Press

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