Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-25T17:14:51.935Z Has data issue: false hasContentIssue false
  • English
  • Français

Developmental changes in proteins and glycoproteins revealed by direct radio-iodination of viable Taenia saginata larvae

Published online by Cambridge University Press:  06 April 2009

G. W. P. Joshua ,
L. J. S. Harrison  and
M. M. H. Sewell
G. W. P. Joshua
Affiliation:
Department of Tropical Animal Health, University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG
L. J. S. Harrison
Affiliation:
Department of Tropical Animal Health, University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG
M. M. H. Sewell
Affiliation:
Department of Tropical Animal Health, University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian EH25 9RG
Get access Rights & Permissions [Opens in a new window]

Summary

Direct surface I radio-isotope labelling techniques and SDS—PAGE analysis were used to compare the proteins and lentil—lectin adherent glycoproteins of the bovine stage of viable Taenia saginata larvae at three points in their development, the invasive oncospheres, immature (4-week-old) and mature (12 to 16-week-old) cysticerci. Some proteins and glycoproteins were present on all three of the ages of the parasite examined but there were also distinct age-specific proteins and glycoproteins detected on oncospheres and 4-week-old cysticerci and a marked difference between the protein/glycoprotein profiles of the parasite was apparent at these earlier stages of development and the mature cysticerci. The latter were characterized by the presence of high, 160–200 kDa molecular weight, lysine rich, glycoproteins, whereas small 16 and 18 kDa glycoproteins and a reduction-sensitive 23 kDa glycoprotein were first detected on 4-week-old immature cysticerci. Antigenic characterization of the isotope-labelled proteins and glycoproteins by immunoprecipitation against a panel of clinically defined bovine sera combined with SDS–PAGE analysis indicated that relatively few proteins were precipitated by sera from T. saginata-infected cattle as compared to the glycoproteins. However, both protein and glycoprotein antigens of possible protective and/or diagnostic significance were identified from oncospheres and cysticerci.

Keywords

Taenia saginatadevelopmentproteinsglycoproteinsantigens
Type
Research Article
Information
Parasitology , Volume 99 , Issue 2 , October 1989 , pp. 265 - 274
Copyright
Copyright © Cambridge University Press 1989

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Anderson, M. J. D. & Griffon, J. F. T. (1979 a). Taenia crassiceps in the rat. I. Differences in susceptibility to infection and development of immunocompetence in relation to age and host strain. International Journal for Parasitology 9, 229–35.CrossRefGoogle Scholar
Anderson, M. J. D. & Griffon, J. F. T. (1979 b). Taenia crassiceps in the rat. II. Transfer of immunity and immunocompetence of lymph node cells. International Journal for Parasitology 9, 235–9CrossRefGoogle Scholar
Bennett, C. E., Joshua, G. W. P. & Hughes, D. L. (1982). Demonstration of juvenile-specific antigens of Fasciola hepatica. Journal of Parasitology 68, 791–5.CrossRefGoogle ScholarPubMed
Bogh, H. O., Rickard, M. D. & Lightowlers, M. W. (1987). Stage-specific immunity following vaccination against Taenia taeniaeformis infection in mice. In Coopers Animal Health Symposium on the Immunobiology and Molecular Biology of Cestode Infections.Google Scholar
Gallie, G. J. & Sewell, M. M. H. (1972). The survival of Cysticercus bovis in resistant calves. The Veterinary Record 91, 481–2.CrossRefGoogle ScholarPubMed
Gallie, G. J. & Sewell, M. M. H. (1974). The serological response of calves infected neonatally with Taenia saginata (Cysticercus bovis). Tropical Animal Health and Production 6, 173–5.CrossRefGoogle Scholar
Gallie, G. J. & Sewell, M. M. H. (1981). Inoculation of calves and adult cattle with oncospheres of Taenia saginata and their resistance to challenge infection. Tropical Animal Health and Production 13, 147–54.CrossRefGoogle ScholarPubMed
Gemmell, M. A. (1986). A critical approach to the concepts of control and eradication of echinococcosis/hydatidosis and taeniasis/cysticercosis. Proceedings of the Sixth International Congress of Parasitology: Parasitology, Quovadit? (ed. Howell, M. J.) pp. 462–472. The Australian Academy of Science.Google Scholar
Gemmell, M. A., Matyas, Z., Pawlowski, Z. & Soulsby, E. J. L. (1983). Guidelines on surveillance, prevention and control of taeniasis/cysticercosis. World Health Organization, VPH 83/49.Google Scholar
Hammerberg, B. & Williams, J. F. (1978). Physicochemical characterisation of complement-interacting factors from Taenia taeniaeformis. Journal of Immunology 120, 1039–45.CrossRefGoogle ScholarPubMed
Hammerber, B., Dangler, C. & Williams, J. F. (1980). Taenia taeniaeformis: chemical composition of parasite factors affecting coagulation and complement cascades. Journal of Parasitology 66, 549–76.Google Scholar
Hannah, R. F. B. (1980). Fasciola hepatica: an immunofluorescent study of antigenic changes in the tegument during development in the rat and sheep. Experimental Parsitology 50, 155–70.CrossRefGoogle Scholar
Harrison, L. J. S., Joshua, G. W. P., Wright, S. H. & Parkhouse, R. M. E. (1989). Specific detection of circulating surface/secreted glycoproteins of viable cysticerci in Taenia saginata cysticercosis. Parasite Immunology 11, 351–70.CrossRefGoogle ScholarPubMed
Harrison, L. J. S. & Parkhouse, R. M. E. (1985). Antigens of taeniid cestodes in protection, diagnosis and escape. Current Topics in Microbiology and Immunology 120, 159–72.Google ScholarPubMed
Harrison, L. J. S. & Parkhouse, R. M. E. (1986). Passive protection against Taenia saginata infection in cattle by a mouse monoclonal antibody reactive with the surface of the invasive oncosphere. Parasite Immunology 8, 319–32.CrossRefGoogle ScholarPubMed
Harrison, L. J. S., Parkhouse, R. M. E. & Sewell, M. M. H. (1984). Variation in ‘target’ antigens between appropriate and inappropriate hosts of Taenia saginata metacestodes. Parasitology 88, 649–63.CrossRefGoogle ScholarPubMed
Joshua, G. W. P., Harrison, L. J. S. & Sewell, M. M. H. (1988). Excreted/secreted products of developing Taenia saginata metacestodes Parasitology 97, 477–87.CrossRefGoogle ScholarPubMed
Jungery, M., Clark, N. W. T. & Parkhouse, R. M. E. (1983). A major change in surface antigens during the maturation of Trichinella spiralis. Molecular and Biochemical Parasitology 7, 101–9.CrossRefGoogle Scholar
Kwa, B. H. & Liew, F. Y. (1977). Immunity in taeniasis/cysticercosis. I. Vaccination against Taenia taeniaeformis in rats using purified antigen. Journal of Experimental Medicine 146, 118–31.CrossRefGoogle ScholarPubMed
Lammas, D. A., Duffus, W. P. H. & Taylor, D. W. (1985). Identification of surface proteins of juvenile stages of Fasciola hepatica. Research in Veterinary Science 38, 248–9.CrossRefGoogle ScholarPubMed
Leid, R. W. (1988). Parasites and complement. Advances in Parasitology 27, 131–58.CrossRefGoogle ScholarPubMed
Letonja, T. & Hammerberg, C. (1987 a). Taenia taeniaeformis early inflammatory response around developing metacestodes in the liver of resistant and susceptible mice. I. Identification of leucocyte response with monoclonal antibodies. Journal of Parasitology 73, 962–70.CrossRefGoogle ScholarPubMed
Letonja, T. & Hammerberg, C. (1987 b). Taenia taeniaeformis early inflammatory response around developing metacestodes in the liver of resistant and susceptible mice. II. Histochemistry and cytochemistry. Journal of Parasitology 73, 971–9.CrossRefGoogle ScholarPubMed
Lloyd, S. (1979). Homologous and heterologous immunization against metacestodes of Taenia saginata and Taenia taeniaeformis in cattle and mice. Zeitschrift für Parasitenkunde 60, 87–96.CrossRefGoogle Scholar
Lloyd, S. & Soulsby, E. J. L. (1976). Passive transfer of immunity to neonatal calves against metacestodes of Taenia saginata. Veterinary Parasitology 2, 355–62.CrossRefGoogle Scholar
Maizels, R. M., Meghji, M. & Ogilvie, B. (1983). Restricted sets of parasite antigens from the surface of different stages and sexes of the nematode parasite Nippostrongylus braziliensis. Immunology 48, 107–20.Google Scholar
Mcmanus, D. P., Mclaren, D. J., Clark, N. W. T. & Parkhouse, R. M. E. (1987). A comparison of two procedures for labelling the surface of the hydatid disease organism, Echinococcus granulosis with 125I. Journal of Helminthology 61, 47–52.CrossRefGoogle Scholar
Mitchell, G. F., Goding, J. W. & Rickard, M. D. (1977). Studies on the immune response to larval cestodes in mice. I. Increased susceptibility in certain mouse strains and hypothymic mice to Taenia taeniaeformis and analysis of passive transfer of resistance with serum. Australian Journal of Experimental Biology and Medical Science 55, 165–86.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E. (1984). Parasite evasion of the immune response. Parasitology 88, 571–82.CrossRefGoogle Scholar
Parkhouse, R. M. E. & Harrison, L. J. S. (1987). Cyst fluid and surface associated glycoprotein antigens of Taenia sp., metacestodes. Parasite Immunology 9, 263–8.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E. & Ortega-Pierres, G. (1984). Stage-specific antigens of Trichinella spiralis. Parasitology 88, 623–30.CrossRefGoogle ScholarPubMed
Parkhouse, R. M. E., Philipp, M. & Ogilvie, B. (1981). Characterisation of surface antigens of Trichinella spiralis infective larvae. Parasite Immunology 3, 339–45.CrossRefGoogle ScholarPubMed
Philipp, M., Parkhouse, R. M. E. & Ogilvie, B. (1980). Changing proteins on the surface of a parasitic nematode. Nature, London 287, 538–40.CrossRefGoogle ScholarPubMed
Pollacco, S., Nicholas, W. L., Mitchell, G. F. & Stewart, A. C. (1978). T-cell dependent collagenous encapsulating response in the mouse liver to Mesocestoides cortii. International Journal for Parasitology 8, 457–62.CrossRefGoogle Scholar
Rajasekariah, G. R., Rickard, M. D. & Mitchell, G. F. (1980). Immunisation of mice against infection with Taenia taeniaeformis using various antigens prepared from eggs, oncospheres, developing larvae and strobilocerci. International Journal for Parasitology 10, 155–60.CrossRefGoogle ScholarPubMed
Rickard, M. D. & Brumley, J. L. (1981). Immunisation of calves against Taenia saginata infection using antigens collected by in vitro incubation of T. saginata oncospheres or ultrasonic disintegration of T. saginata and Taenia hydatigena oncospheres. Research in Veterinary Science 30, 99–103.CrossRefGoogle ScholarPubMed
Rickard, M. D. & Outeridge, P. M. (1974). Antibody and cell-mediated immunity in rabbits infected with the larval stage of Taenia pisiformis. Zeitschrift für Parasitenkunde 44, 187–201.CrossRefGoogle ScholarPubMed
Rickard, M. D. & Williams, J. F. (1982). Hydatidosis/Cysticercosis: Immune mechanisms and immunisation against infection. Advances in Parasitology 21, 230–96.Google ScholarPubMed
Rogers, A. W. (1979). Practical Autoradiography. Amersham International plc. Review No. 20.Google Scholar
Slais, J. (1970). The morphology and pathogenicity of the bladder worms Cysticercus cellulosae and Cysticercus bovis. Pubs. Academica; Publishing House of the Czechoslovak Academy of Sciences, Prague.CrossRefGoogle Scholar
Siebert, A. E., Good, A. H. & Simmons, J. E. (1978 a). Kinetics of primary and secondary infection with Taenia crassiceps metacestodes. International Journal for Parasitology 8, 39–43.CrossRefGoogle Scholar
Siebert, A. E., Good, A. H. & Simmons, J. E. (1978b). Ultrasonic aspects of early immune damage to Taenia crassiceps metacestodes. International Journal for Parasitology 8, 45–53.CrossRefGoogle Scholar
Smithers, S. R. (1986). Vaccination against schistosomes and other systemic helminths. In Parasitology: Quo vadit Proceedings of the 6th International Congress of Parasitology, pp. 31–43.Google Scholar
Williams, J. F. (1982). Cestode infections. In Immunology of Parasitic Infections (ed. Cohen, S. & Warren, K. S.), pp. 676–714. Oxford: Blackwell Scientific Publications.Google Scholar
Zingales, B. (1984). Labelling of trypanosome surface antigens by lactoperoxidase and Iodo-gen. In Genes and Antigens of Parasites: a Laboratory Manual. 2nd Edn. (ed. Morel, C.), pp. 333–8. Fundacio Oswaldo Cruz.Google Scholar