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Development of Plasmodium berghei ookinetes in the midgut of Anopheles atroparvus mosquitoes and in vitro

Published online by Cambridge University Press:  06 April 2009

C. J. Janse ,
R. J. Rouwenhorst ,
P. F. J. Van Der Klooster ,
H. J. Van Der Kaay  and
J. P. Overdulve
C. J. Janse
Affiliation:
Department of Tropical Veterinary Medicine and Protozoology, State University of Utrecht, P.O. Box 80.172, 3508 TD Utrecht, The Netherlands
R. J. Rouwenhorst
Affiliation:
Laboratory of Parasitology (Medical Faculty), State University of Leiden, Wassenaarseweg 62, 2333 AL Leiden, The Netherlands
P. F. J. Van Der Klooster
Affiliation:
Department of Tropical Veterinary Medicine and Protozoology, State University of Utrecht, P.O. Box 80.172, 3508 TD Utrecht, The Netherlands
H. J. Van Der Kaay
Affiliation:
Laboratory of Parasitology (Medical Faculty), State University of Leiden, Wassenaarseweg 62, 2333 AL Leiden, The Netherlands
J. P. Overdulve
Affiliation:
Department of Tropical Veterinary Medicine and Protozoology, State University of Utrecht, P.O. Box 80.172, 3508 TD Utrecht, The Netherlands
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Extract

Plasmodium berghei ookinete formation in vitro and within the midgut of susceptible Anopheles atroparvus were compared. No significant morphological differences were seen, except that in vitro development was more synchronized and less degenerating forms occurred. In vitro ookinete yields were 4–31 times higher and less variable than those in vivo. Mosquitoes of a susceptible and of a refractory line of A. atroparvus were simultaneously fed on the same host or via a membrane with the same suspension of in vitro-formed mature ookinetes. Up to 100% of mosquitoes of the susceptible line produced oocysts, mostly in high numbers, whereas infection rates and numbers of oocysts produced in mosquitoes of the refractory line were lower and much more so after host feeding than after membrane feeding of mature ookinetes, indicating that refractoriness does not depend on a single process of inhibition.

Type
Research Article
Information
Parasitology , Volume 91 , Issue 2 , October 1985 , pp. 219 - 225
Copyright
Copyright © Cambridge University Press 1985

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