Hostname: page-component-78c5997874-m6dg7 Total loading time: 0 Render date: 2024-11-19T13:16:45.518Z Has data issue: false hasContentIssue false
  • English
  • Français

Development of larval Echinococcus granulosus confronted with chicken heart tissue in vitro

Published online by Cambridge University Press:  06 April 2009

D. Janssen ,
P. H. De Rycke  and
L. De Ridder
D. Janssen
Affiliation:
Laboratory of Zoophysiology, State University of Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
P. H. De Rycke
Affiliation:
Laboratory of Zoophysiology, State University of Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
L. De Ridder
Affiliation:
Laboratory of Histology, State University of Gent, L. Pasteurlaan 2, B-9000 Gent, Belgium
Get access Rights & Permissions [Opens in a new window]

Summary

Vesiculated protoscoleces (VP) were produced by culturing freshly collected protoscoleces from Echinococcus granulosus horse liver hydatids in RPMI 1640 monophasic medium at 37°C for 18 days. Half of the VP were used as such, the other half used after killing them by freeze—thawing. Nine-day-old chicken heart fragments (CHF) were cultured in MEM at 37°C for 72 h. Subsequently, CHF were put together with live and dead VP, respectively, for up to 53 days, on a semisolid medium consisting of agar, Ringer's and MEM. Time-dependent histological observations revealed that dead VP were surrounded by CHF cells. Dead VP tissue was eventually internalized and disintegrated in about 1 week. Live VP penetrated into the CHF tissue and further developed into small hydatid cysts, located within the boundaries of the experimental ‘host’ tissue. The amorphous-looking contact region PAP-stained positively only with anti-E. granulosus serum and not with anti-CHF serum; it was considered identical to the normal laminated layer. The invasion of VP in CHF tissue proved to be different from a tumour or a bacterial invasion: it was concluded that the confrontation of VP and CHF had resulted in an ‘in vitro cohabitation’ rather than in an ‘in vitro infecion’.

Keywords

Echinococcus granulosuslarval developmentin vitrochicken heart
Type
Research Article
Information
Parasitology , Volume 100 , Issue 3 , June 1990 , pp. 453 - 458
Copyright
Copyright © Cambridge University Press 1990

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

De Rycke, P. H. & Pennoit-De Cooman, E (1978). Serial passages of larval Echinococcus granulosus from equine origin in mice. I. Infection with protoscoleces. Zeitschrift für Parasitenkunde 55, 229–34.CrossRefGoogle ScholarPubMed
De Wit, M. (1987). Study on the occurrence of subspeciation in Echinococcus granulosus (hydatidosis) in Belgium [In Flemish: Onderzoek naar het voorkomen van subspeciatie van Echinococcus granulosus (hydatidosis) in België]. Thesis, State University of Gent, Belgium.Google Scholar
Fried, D. D. (1989). Cultivation of trematodes in chick embryos. Parasitology Today 5, 3–5.CrossRefGoogle ScholarPubMed
Heath, D. D. & Osborn, P. J. (1976). Formation of Echinococcus granulosus laminated membrane in a defined medium. International Journal for Parasitology 6, 467–71.CrossRefGoogle Scholar
Howell, M. J. (1986). Cultivation of Echinococcus species in vitro. In The Biology of Echinococcus and Hydatid Diseases. (Thompson, R. C. A.), pp. 143–163London: George Allen & Unwin.Google Scholar
Mareel, M.Kint, J. & Meyvisch, C. (1979). Method of study of the invasion of malignant C3H mouse fibroblasts into embryonic chick heart in vitro. Virchows Archives B. Cell Pathology 30, 95–111.CrossRefGoogle ScholarPubMed
Osuna, A.Gamarro, F.Janssen, D.Castanys, S. & De Rycke, P. H. (1985). Echinococcus granulosus: cytotoxic effects of hydatid cyst fluid on intermediate host cells.. Annales de la Société belge de Médecine tropicale 65, 410.Google Scholar
Richards, K. S.Arme, C. & Bridges, J. F. (1983). Echinococcus granulosus equinus: an ultrastructural study of the laminated layer, including changes on incubating cysts in various media.. Parasitology 86, 399–405.CrossRefGoogle ScholarPubMed
Rogan, M. T. & Richards, K. S. (1986). In vitro development of hydatid cysts from posterior bladders and ruptured brood capsules of equine Echinococcus granulosus. Parasitology 92, 379–90.CrossRefGoogle ScholarPubMed
Romeis, B. (1968). Mikroskopische Technik. Müuchen: R. Oldenburg Verlag.Google Scholar
Smyth, J. D. (1962). Studies on tapeworm physiology. X. Axenic cultivation of the hydatid organism, Echinococcus granulosus: establishment of a basic technique.. Parasitology 52, 441–57.CrossRefGoogle Scholar
Smyth, J. D. & Davies, Z. (1974). Occurrence of physiological strains of Echinococcus granulosus demonstrated by in vitro culture of protoscoleces from sheep and horse hydatid cysts. International Journal for Parasitology 4, 443–5.CrossRefGoogle ScholarPubMed
Smyth, J. D.Howkins, A. B. & Barton, B. (1966). Factors controlling the differentiation of the organism Echinococcus granulosus, into cystic or strobilate stages in vitro. Nature London 211, 1374–7.CrossRefGoogle ScholarPubMed
Stenberger, L. A. (1974). Immunocytochemistry. Englewood Cliffs, New York: Prentice Hall. Inc.Google Scholar
Voge, M. (1978). Cestoda. In The Cultivation of Parasites in Vitro, 2nd. EdnTaylor, A. E. R. & Baker, J. R.), pp. 193–225Oxford: Blackwell Scientific Publications.Google Scholar
Wanson, J.-C., De Ridder, L. & Mosselmans, R. (1981). Invasiveness of hyperplastic nodule cells from diethylnitrosamine treated rat liver. Cancer Research 41, 5162–75.Google ScholarPubMed
Wardle, R. A. (1934). The viability of tapeworms in artificial media.. Physiological Zoology 7, 36–61.CrossRefGoogle Scholar