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The development of Haemonchus contortus in vitro

I. The effect of pH and pCO2 on the rate of development to the fourth-stage larva

Published online by Cambridge University Press:  06 April 2009

C. J. Mapes
C. J. Mapes
Affiliation:
Parasitology Department, Moredun Research Institute, Edinburgh 9
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Extract

The development of the exsheathed third-stage larva of Haemonchus contortus to the fourth stage in vitro has been investigated in half-strength Ringer's solution and in solutions with ionic concentrations similar to those found in the abomasum during normal secretion of acid and during heavy infestations of Haemonchus. The concentration of carbon dioxide required for the optimum development of the larva has been shown to be dependent on the pH of the medium. At a low pH the optimum pC02 is low and the larva is less sensitive to a range of carbon dioxide concentrations than at a pH near neutrality. Gastric mucin and histamine have been shown to have an inhibiting and a potentiating effect, respectively, on development, in media at pH 6.

Type
Research Article
Information
Parasitology , Volume 59 , Issue 1 , February 1969 , pp. 215 - 231
Copyright
Copyright © Cambridge University Press 1969

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References

REFERENCES

Anderson, N., Armour, J., Rosalind, M., Jarrett, W. F. H., Jennings, F. W., Ritchie, J. S. D. & Urquhart, G. M. (1966). Experimental Ostertagia infections in calves; results of single infections with five graded dose levels of larvae. Am. J. vet. Res. 27, 1259–65.Google Scholar
Ash, R. W. & Kay, R. N. B. (1963). Digestive secretions and the flow of food material in the sheep. In Progress in Nutrition and Allied Sciences. Ed. Cuthbertson, D. P.Edinburgh and London: Oliver and Boyd.Google Scholar
Armour, J., Jarrett, W. F. H. & Jennings, F. W. (1966). Experimental Ostertagia circumcincta infections in sheep. Am. J. vet. Res. 27, 1267–78.Google Scholar
Christie, M. G., Brambell, M. R. & Mapes, C. J. (1967). Effect of young Haemonchus on abomasal pH in the sheep. Vet. Rec. 80, 207.Google Scholar
Christie, M. G. & Patterson, J. E. (1963). Improved methods of obtaining clean larva of Haemonchus contortus. J. Helminth. 37, 251–4.CrossRefGoogle ScholarPubMed
Diamond, L. S. & Douvres, F. W. (1962). Bacteria free cultivation of some parasitic stages of the swine nematode Hyostrongylus rubidus and Oesophagostomum quadrispinulatum (O. longicaudum). J. Parasit. 48, 39–42.CrossRefGoogle ScholarPubMed
Hill, K. J. (1965). Abomasal secretory function in the sheep. In Physiology of Digestion in the Ruminant Ed. Dougherty, R. W.et al. London: Butterworths.Google Scholar
Lapage, G. (1935). The behaviour of sterilized exsheathed infective Trichostrongylid larvae in sterile media resembling their environment in the ovine host. J. Helminth. 13, 115–28.CrossRefGoogle Scholar
Leland, S. E. (1963). Studies on the in vitro growth of parasitic nematodes. I. Complete or partial parasitic development of some gastrointestinal nematodes of sheep and cattle. J. Parasit. 49, 600–11.CrossRefGoogle ScholarPubMed
Oyeart, W. & Boukaert, J. H. (1961). A study of the passage of fluid through the sheep's omasum. Res. vet. Sci. 2, 41–52.CrossRefGoogle Scholar
Read, C. P. (1950). The vertebrate small intestine as an environment for parasitic helminths. Rice Inst. Pamph. 37 (2), 1–94.Google Scholar
Rogers, W. P. (1960). The physiology of infective processes of nematode parasites; the stimulus of the animal host. Proc. R. Soc. B 152, 367–86.Google ScholarPubMed
Rogers, W. P. (1966 a). The reversible inhibition of exsheathment in some parasitic nematodes. Comp. Biochem. Physiol. 17, 1103–10.CrossRefGoogle Scholar
Rogers, W. P. (1966 b). Reversible inhibition of a receptor governing infection with some nematodes. Expl Parasit. 19, 15–20.CrossRefGoogle ScholarPubMed
Schultz, H. P. (1967). Versuche zur Kultivierung der parasitischen Larvenstadien von Haemonchus contortus in vitro. Berl. Münch. tierärztl. Wschr. 80, 89–96.Google Scholar
Sommerville, R. I. (1964). Effect of carbon dioxide on the development of third stage larvae of Haemonchus contortus in vitro. Nature, Lond. 202, 316–7.CrossRefGoogle ScholarPubMed
Sommerville, R. I. (1966). The development of Haemonchus contortus to the fourth stage in vitro. J. Parasit. 52, 127–36.CrossRefGoogle Scholar
Turner, A. W. & Hodgetts, V. E. (1955). Buffering systems in the rumen of the sheep. I. pH and bicarbonate concentration in relationship to pCO2. Aust. J. agric. Res. 6, 115–24.CrossRefGoogle Scholar
Weinstein, P. P. & Jones, M. F. (1956). The in vitro cultivation of Nippostrongylus muris to the adult stage. J. Parasit. 42, 215–36.CrossRefGoogle Scholar