Hostname: page-component-78c5997874-xbtfd Total loading time: 0 Render date: 2024-11-05T04:49:15.716Z Has data issue: false hasContentIssue false
  • English
  • Français

Detection of polymorphisms among Theileria parva stocks using repetitive, telomeric and ribosomal DNA probes and anti-schizont monoclonal antibodies

Published online by Cambridge University Press:  06 April 2009

R. P. Bishop ,
B. K. Sohanpal ,
B. A. Allsopp ,
P. R. Spooner ,
T. T. Dolan  and
S. P. Morzaria
R. P. Bishop
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
B. K. Sohanpal
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
B. A. Allsopp
Affiliation:
University of Cambridge, Department of Biochemistry, Tennis Court Road, Cambridge CB2 1QW, U.K.
P. R. Spooner
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
T. T. Dolan
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
S. P. Morzaria
Affiliation:
International Laboratory for Research on Animal Diseases, P.O. Box 30709, Nairobi, Kenya
Get access Rights & Permissions [Opens in a new window]

Summary

A total of 21 Theileria parva stocks from 6 countries were characterized using T. parva repetitive and ribosomal DNA probes, a Plasmodium berghei telomeric oligonucleotide and a panel of anti-schizont monoclonal antibodies (MAbs). Hybridization of the repetitive DNA probe to Southern blots of EcoRI-digested T. parva DNA revealed 20 different restriction fragment patterns among DNA samples isolated from infections initiated using 16 parasite stocks. The panel of anti-schizont MAbs defined 8 different profiles among schizont-infected lymphoblastoid cell-cultures infected with the same 16 T. parva stocks. Many stocks, which were differentiated by the repetitive DNA probe, could not be distinguished using the anti-schizont MAbs. A cloned T. parva small subunit ribosomal RNA (SSUrRNA) gene probe separated 17 T. parva stocks into 2 groups, exhibiting either 1 or 2 restriction fragments, when hybridized to EcoRI-digested T. parva DNA. When hybridized to PvuII-digested DNA from 8 T. parva stocks, the ribosomal probe identified 4 groups with similar restriction fragment patterns. A synthetic oligonucleotide derived from a P. berghei telomeric sequence hybridized to 7 or 8 size-polymorphic restriction fragments in the EcoRI-digested DNA of most T. parva stocks. The telomeric and ribosomal probes defined the same 4 groups among 8 T. parva stocks as assessed by similarities in restriction fragment patterns. Based on the comparison of repetitive DNA sequences from the T. parva Uganda and Muguga stocks, a synthetic oligonucleotide was developed which distinguished the DNA of the T. parva Uganda stock from that of 4 other T. parva stocks on a positive/negative basis.

Keywords

Theileria parvaDNA proberestriction fragment length polymorphism (RFLP)synthetic oligonucleotide
Type
Research Article
Information
Parasitology , Volume 107 , Issue 1 , July 1993 , pp. 19 - 31
Copyright
Copyright © Cambridge University Press 1993

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Allsopp, B. A. & Allsopp, M. T. E. P. (1988). Theileria parva: genomic DNA studies reveal intra-specific sequence diversity. Molecular and Biochemical Parasitology 28, 77–84.CrossRefGoogle ScholarPubMed
Allsopp, B. A., Baylis, H. A., Allsopp, M. T. E. P., Cavalier-Smith, T., Bishop, R. P., Carrington, M. D., Sohanpal, B. & Spooner, P. R. (1993). Discrimination between six species of Theileria using oligonucleotide probes which detect small subunit ribosomal RNA sequences. Parasitology 107 (in the Press.)CrossRefGoogle ScholarPubMed
Allsopp, B., Carrington, M., Baylis, H., Sohal, S., Dolan, T. & Iams, K. (1989). Improved characterisation of Theileria parva isolates using the polymerase chain reaction and oligonucleotide probes. Molecular and Biochemical Parasitology 35, 137–48.CrossRefGoogle ScholarPubMed
Allsopp, B. A., Gibson, W. C., Stagg, D. A. (1985). Characterisation of some East African Theileria species isolates by isoenzyme analysis, with particular reference to T. parva. International Journal for Parasitology 15, 271–6.CrossRefGoogle ScholarPubMed
Baylis, H. A., Sohal, S. K., Carrington, M., Bishop, R. P. & Allsopp, B. A. (1991). An unusual repetitive gene family in Theileria parva which is stage specifically transcribed. Molecular and Biochemical Parasitology 49, 133–42.CrossRefGoogle ScholarPubMed
Biggs, B. A., Kemp, D. J. & Brown, D. V. (1989). Subtelomeric deletions in field isolates of Plasmodium faliparum and their relationship to loss of cytoadherence in vitro. Proceedings of the National Academy of Sciences, USA 86, 2428–32.CrossRefGoogle Scholar
Brocklesby, D. W., Barnett, S. F. & Scott, G. R. (1961). Morbidity and mortality rates in East coast fever (Theileria parva infection) and their application to drug screening procedures. British Veterinary Journal 17, 529–31.CrossRefGoogle Scholar
Brown, C. G. D. (1979). Propagation of Theileria. In Practical Tissue Culture Applications (ed. Maramorosch, K. & Hirum, H.), pp. 223–245. New York: Academic Press.Google Scholar
Brown, W. C. & Grab, D. J. (1985). Biological and biochemical characterisation of bovine interleukin 2. Studies with cloned bovine T cells. Journal of Immunology 133, 3184–9.CrossRefGoogle Scholar
Conrad, P. A., Baldwin, C. L., Brown, W. C., Sohanpal, B. A., Dolan, T. T., Goddeeris, B. M., Demartini, J. C. & Ole-Moiyoi, O. K. (1989 b). Infection of bovine T cell clones with genotypically distinct Theileria parva parasites and analysis of their cell surface phenotype. Parasitology 99, 205–31.CrossRefGoogle Scholar
Conrad, P. A., Iams, K., Brown, W. C., Sohanpal, B. & Ole-Moi Yoi, O. (1987 b). DNA probes detect genomic diversity in Theileria parva stocks. Molecular and Biochemical Parasitology 25, 213–26.CrossRefGoogle ScholarPubMed
Conrad, P. A., Ole-Moiyoi, O. K., Baldwin, C. L., Dolan, T. T., O'callaghan, C. J., Njamunggeh, R. E. G., Grootenhuis, J. G., Stagg, D. A., Leitch, B. L. & Young, A. S. (1989 a). Characterisation of buffalo-derived theilerial parasites with monoclonal antibodies and DNA probes. Parasitology 98, 179–88.CrossRefGoogle ScholarPubMed
Conrad, P. A., Stagg, D. A., Grootenhuis, J. G., Irvin, A. D., Newson, R. M., Njammungeh, R. E. G., Rossiter, P. B. & Young, A. S. (1987 a). Isolation of Theileria parasites from African buffalo (Syncerus caffer) and characterization with anti-schizont monoclonal antibodies. Parasitology 94, 413–23.CrossRefGoogle ScholarPubMed
Corcoran, L. M., Thompson, J. K., Walliker, D. & Kemp, D. J. (1988). Homologous recombination within subtelomeric repeat sequences generates chromosome size polymorphisms in Plasmodium falciparum. Cell 53, 807–13.CrossRefGoogle Scholar
Dalrymple, B. P. (1990). Cloning and characterisation of the rRNA genes and flanking regions from Babesia bovis: use of the genes as strain discriminating probes. Molecular and Biochemical Parasitology 43, 117–24.CrossRefGoogle ScholarPubMed
Dietrich, P., Pilar Dussan, M., Floeter-Winter, L. M., Trebilcock, M. H., Camargo, E. P. & Bento Soares, M. (1990). Restriction fragment length polymorphisms in the ribosomal gene spacers of Trypanosoma cruzi and Trypanosoma conorhini. Molecular and Biochemical Parasitology 42, 13–20.CrossRefGoogle ScholarPubMed
Dolan, T. T., Radley, D. E., Brown, C. G. D., Cunningham, M. P., Morzaria, S. P. & Young, A. S. (1980). East Coast fever: further studies on the protection of cattle immunised with a combination of theilerial strains. Veterinary Parasitology 6, 325–32.CrossRefGoogle Scholar
Higgins, D. G. & Sharp, P. M. (1988). CLUSTAL: a package for performing multiple sequence alignments on a microcomputer. Gene 73, 237–44.CrossRefGoogle ScholarPubMed
Irvin, A. D. (1987). Characterisation of species and strains of Theileria. Advances in Parasitology 26, 145–79.CrossRefGoogle ScholarPubMed
Irvin, A. D., Dobbelaere, D. A. E., Mwamachi, D. M., Minami, M., Spooner, P. R. & Ocama, J. G. R. (1983). Immunisation against East Coast fever: Correlation between monoclonal antibody profiles of Theileria parva stocks and cross-immunity in vivo. Research in Veterinary Science 35, 341–6.CrossRefGoogle ScholarPubMed
Lawrence, J. A. & Mackenzie, P. K. I. (1980). Isolation of a non-pathogenic Theileria of cattle transmitted by Rhipicephalus appendiculatus. Zimbabwe Veterinary Journal 11, 27–35.Google Scholar
Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: a Laboratory Manual. Cold Spring Harbour, New York: Cold Spring Harbour Laboratory.Google Scholar
Mehlhorn, H. & Schein, E. (1984). The piroplasms: life cycle and sexual stages. Advances in Parasitology 23, 37–103.CrossRefGoogle ScholarPubMed
Melrose, T. R. & Brown, C. G. D. (1979). Isoenzyme variation in piroplasms isolated from bovine blood infected with Theileria annulata and T. parva. Research in Veterinary Science 27, 379–81.CrossRefGoogle ScholarPubMed
Minami, T., Spooner, P. R., Irvin, A. D., Ocama, J. G. R., Dobbelaere, D. A. E. & Fujinaga, T. (1983). Characterisation of stocks of Theileria parva by monoclonal antibody profiles. Research in Veterinary Science 35, 334–40.CrossRefGoogle ScholarPubMed
Morzaria, S. P., Spooner, S. P., Bishop, R. P., Musoke, A. J. & Young, J. R. (1990). SfiI and NotI polymorphisms in Theileria stocks detected by pulsed field gel electrophoresis. Molecular and Biochemical Parasitology 40, 203–12.CrossRefGoogle ScholarPubMed
Morzaria, S. P. & Young, J. R. (1992). Restriction mapping of the genome of the protozoan parasite Theileria parva. Proceedings of the National Academy of Sciences, USA 89, 5241–5.CrossRefGoogle ScholarPubMed
Mutugi, J. J., Young, A. S., Maritim, A. C., Ndungu, S. K., Mining, S. K., Linyonyi, A., Ngumi, P. N., Leitch, B. L., Morzaria, S. P. & Dolan, T. T. (1989). Immunization of cattle in Coast Province Kenya: laboratory evaluation of a Theileria parva parva stabilate for use in infection and treatment immunization in the field. Research in Veterinary Science 27, 170–7.CrossRefGoogle Scholar
Ponzi, M., Pace, T., Dore, E. & Frontali, C. (1985). Identification of a telomeric DNA sequence in Plasmodium berghei. The EMBO Journal 4, 2991–5.CrossRefGoogle ScholarPubMed
Radley, D. E., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Kirimi, I. M., Purnell, R. E. & Young, A. S. (1975). East coast fever, 1. Chemoprophylactic immunization of cattle against Theileria parva (Muguga) and five theilerial strains. Veterinary Parasitology 1, 35–41.CrossRefGoogle Scholar
Shapiro, S. Z., Fujisaki, K., Morzaria, S. P., Webster, P., Fujinaga, T., Spooner, P. R. & Irvin, A. D. (1987). A life-cycle stage-specific antigen of Theileria parva recognized by anti-schizont monoclonal antibodies. Parasitology 94, 29–37.CrossRefGoogle Scholar
Stagg, D. A., Young, A. S., Leitch, B. L., Grootenhuis, J. G. & Dolan, T. T. (1983). Infection of mammalian cells with Theileria species. Parasitology 86, 243–54.CrossRefGoogle ScholarPubMed
Sugimoto, C., Conrad, P. A., Mutharia, L., Dolan, T. T., Brown, W. C., Goddeeris, B. M. & Pearson, T. W. (1989). Phenotypic characterisation of Theileria parva schizonts by two-dimensional gel electrophoresis. Parasitology Research 76, 1–7.CrossRefGoogle ScholarPubMed
Toye, P. G., Goddeeris, B. M., Iams, K., Musoke, A. J. & Morrison, W. I. (1991). Characterisation of a polymorphic immunodominant molecule in sporozoites and schizonts of Theileria parva. Parasite Immunology 13, 49–62.CrossRefGoogle ScholarPubMed
Uilenberg, G. (1981). Theilerial species of domestic livestock. In Advances in the Control of Theileriosis (ed. Irvin, A. D., Cunningham, M. P. & Young, A. S.), pp. 4–37. The Hague: Martinus Nijhoff.CrossRefGoogle Scholar
Young, A. S., Brown, C. G. D., Burridge, M. J., Cunningham, M. P., Kirimi, I. M. & Irvin, A. D. (1973). Observations on the cross-immunity between Theileria lawrencei (Serengeti) and Theileria parva (Muguga) in cattle. International Journal for Parasitology 3, 723–8.CrossRefGoogle ScholarPubMed