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Description of inflammation and cytokine profile at the inoculation site and in heart tissue of mice re-infected with Trypanosoma cruzi vector derived-metacyclic trypomastigotes

Published online by Cambridge University Press:  06 January 2005

L. GÓMEZ-GARCÍA
Affiliation:
Laboratorio Inmunoparasitología, Instituto Nacional Cardiología “Ignacio Chávez”, México DF
R. ALEJANDRE-AGUILAR
Affiliation:
Departamento Parasitología, Escuela Nacional Ciencias Biológicas, IPN, México DF
A. ARANDA-FRAUSTRO
Affiliation:
Laboratorio Inmunoparasitología, Instituto Nacional Cardiología “Ignacio Chávez”, México DF
R. LOPEZ
Affiliation:
Centro de Investigación en Enfermedades Tropicales, Universidad Autónoma de Campeche, México
V. M. MONTEÓN
Affiliation:
Centro de Investigación en Enfermedades Tropicales, Universidad Autónoma de Campeche, México

Abstract

We studied the role of Trypanosoma cruzi reinfection in regard to inflammatory and cytokine response at the inoculation site, lymph node and heart. We reinfected Balb/c mice intradermically into the hind foot-pad with natural infective metacyclic trypomastigotes. They were followed from 24 h to 30 days after the last reinfection. At the inoculation site 24 h after the last re-infection, the infiltrating inflammatory cells increased dramatically with respect to baseline inflammation, reaching maximum infiltrates for the third day. In contrast, parasite DNA was undetectable 24 h after inoculation, despite poor cytokine induction, only IFN-gamma, IL-12 and TGF-β were noticeable on days 7 and 15, whereas in the lymph nodes draining the inoculation site positive expression of IL-2, IL-4, IL-12 and TGF-beta were found to be induced as soon as 24 h after re-entry of parasite. In the heart, the inflammatory response increased immediately 24 h after re-entry of parasites, reaching its maximum on the 7th day and returning to baseline on day 30. In conclusion, although the inflammatory response is triggered in both compartments by re-entry of parasites, the inflammatory process returns almost to baseline after 30 days, leaving a persistent low-grade inflammation.

Type
Research Article
Copyright
© 2005 Cambridge University Press

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