Hostname: page-component-586b7cd67f-2brh9 Total loading time: 0 Render date: 2024-11-26T05:21:15.489Z Has data issue: false hasContentIssue false
  • English
  • Français

Cytogenetic and sequence comparison of adult Phyllodistomum (Digenea: Gorgoderidae) from the three-spined stickleback with larvae from two bivalves

Published online by Cambridge University Press:  18 November 2004

R. PETKEVIČIŪTĖ ,
V. STUNŽĖNAS  and
G. STANEVIČIŪTĖ
R. PETKEVIČIŪTĖ
Affiliation:
Institute of Ecology, Vilnius University, Akademijos 2, LT-08412, Vilnius 21, Lithuania
V. STUNŽĖNAS
Affiliation:
Institute of Ecology, Vilnius University, Akademijos 2, LT-08412, Vilnius 21, Lithuania
G. STANEVIČIŪTĖ
Affiliation:
Institute of Ecology, Vilnius University, Akademijos 2, LT-08412, Vilnius 21, Lithuania
Get access Rights & Permissions [Opens in a new window]

Abstract

Due to the low informative value of available morphological characters, cytogenetic and molecular methods, based on rDNA sequencing, were used to characterize adult and larval stages of Phyllodistomum spp. Species studied have 18 chromosomes with comparable absolute and relative lengths. Conventional Giemsa staining and karyometric analysis revealed clear differences in chromosome morphology of larval Phyllodistomum spp. infecting two bivalve host species, Sphaerium corneum and Pisidium amnicum. However, karyotypes of adult P. folium from three-spined sticklebacks and larval stages from S. corneum appear almost identical both with respect to the relative lengths and centromeric indices of the corresponding chromosome pairs. The entire internal transcribed spacer (ITS) region (ITS-1, 5.8S and ITS-2) and the D1-D3 region of 28S gene were sequenced and compared. Again, sufficient differences were observed between larval Phyllodistomum spp., while adult P. folium and larvae from S. corneum showed a high level of similarity. So, both cytogenetic and molecular data support the suggestion that they represent developmental stages of the same species. The results were compared with published data obtained by cytogenetic and molecular studies on the other Phyllodistomum species. Differences revealed in karyotype and rDNA sequences leads to the conclusion that the cercariaeum of P. folium sensu Sinitsin, 1905 could not be regarded as the larva of adult P. folium from three-spined stickleback.

Keywords

PhyllodistomumTrematodakaryotypechromosomesribosomal DNAlife-cycles
Type
Research Article
Information
Parasitology , Volume 129 , Issue 6 , December 2004 , pp. 771 - 778
Copyright
© 2004 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

BLAIR, D., BRAY, R. A. & BARKER, S. C. ( 1998). Molecules and morphology in phylogenetic studies of the Hemiuroidea (Digenea: Trematoda: Platyhelminthes). Molecular Phylogenetics and Evolution 9, 15–25.CrossRefGoogle Scholar
BYKHOVSKAYA-PAVLOVSKAYA, I. E. & KULAKOVA, A. P. ( 1987). Trematoda. In Key to Parasites of Freshwater Fish of USSR (ed. Bauer, O. N.), 3, pp. 77–198. Nauka, Leningrad. (In Russian.)
CHAPPELL, L. H. ( 1969). The parasites of the three-spined stickleback Gasterosteus aculeatus L. from a Yorkshire pond. I. Seasonal variation of parasite fauna. Journal of Fish Biology 1, 137–152.Google Scholar
CRIBB, T. H., ANDERSEN, G. R., ADLARD, R. D. & BRAY, R. A. ( 1998). A DNA-based demonstration of a three-host life cycle for the Bivesiculidae (Plathelminthes: Digenea). International Journal for Parasitology 28, 1791–1795.CrossRefGoogle Scholar
DUGAROV, Zh. N. ( 2000). Morphological variability in adults of Phyllodistomum umblae and P. folium (Trematoda: Gorgoderidae) from fishes of the Baikal basin. Parazitologya 34, 315–322. (In Russian.)Google Scholar
DAWES, B. ( 1946). The Trematoda. Cambridge University Press, Cambridge, UK.
DHINGRA, O. P. ( 1954). Taxonomic values of chromosomes and cytoplasmic inclusions in a digenetic trematode – Phyllodistomum spatula. Research Bulletin of the Panjab University 51, 101–109.Google Scholar
DVORÁK, J., VANÁCOVÁ, S., HAMPL, V., FLEGR, J. & HORÁK, P. ( 2002). Comparison of European Trichobilharzia species based on ITS1 and ITS2 sequences. Parasitology 124, 307–313.CrossRefGoogle Scholar
GINETZINSKAYA, T. A. ( 1959). On cercarian fauna of molluscs from Rybinsk water reservoir. In Ecological Parasitology (ed. Polianskij, J. I.), pp. 96–149. University Press, Leningrad. (In Russian.)
JOUSSON, O., BARTOLI, P. & PAWLOWSKI, J. ( 1999). Molecular identification of developmental stages in Opecoelidae (Digenea). International Journal for Parasitology 29, 1853–1858.CrossRefGoogle Scholar
JOUSSON, O., BARTOLI, P., ZANINETTI, L. & PAWLOWSKI, J. ( 1998). Use of the ITS rDNA for elucidation of some life-cycles of Mesometridae (Trematoda, Digenea). International Journal for Parasitology 28, 1403–1411.CrossRefGoogle Scholar
KOZICKA, J. ( 1959). Parasites of fishes of Druzno Lake. Part VIII. Acta Parasitologica Polonica 7, 1–72.Google Scholar
KOVAL, V. P. ( 1978). Trematodes of the genus Phyllodistomum Braun, 1899 of freshwater fish of Ukraina. In Scientific and Applied Helminthological Problems (ed. Jershov, V. S.), pp. 48–52. Nauka, Moskow. (In Russian.)
KENNEDY, C. R. ( 1974). A checklist of British and Irish freshwater fish parasites with notes on their distribution. Journal of Fish Biology 6, 613–644.CrossRefGoogle Scholar
KUDINOVA, M. A. ( 1994). On the revision of system of the trematode genus Phyllodistomum Braun, 1899 (Gorgoderidae). In Ecological Parasitology (ed. Shulman, S. S.), pp. 96–112. Kaulian Research Center RAS, Petrozavodsk. (In Russian.)
LEVAN, A., FREDGA, K. & SANDBERG, A. ( 1964). Nomenclature for centromere position on chromosomes. Hereditas 52, 201–220.Google Scholar
LEWIS, F. J. ( 1935). The trematode genus Phyllodistomum Braun. Transactions of the American Microscopical Society 54, 103–117.CrossRefGoogle Scholar
MORGAN, J. A. & BLAIR, D. ( 1998). Mitochondrial ND1 gene sequences used to identify echinostome isolates from Australia and New Zealand. International Journal for Parasitology 28, 493–502.CrossRefGoogle Scholar
NIE, P. & KENNEDY, C. R. ( 1992). Occurrence of Phyllodistomum folium (Olfers) (Digenea) in the eel, Anguilla anguilla (Linnaeus), in a small river. Journal of Fish Biology 40, 469–470.CrossRefGoogle Scholar
NYBELIN, O. ( 1926). Zur Helminthenfauna der Süsswasser Fische Schwedens I. Phyllodistomen. Göteborgs K. Vetenskaps och Vitterhets Samhälles Handlinger 31, 1–29.Google Scholar
ODHNER, T. ( 1911). Zum natürlichen System der digenen Trematoden IV. Zoologischer Anzeiger 38, 513–531.Google Scholar
ORECCHIA, P., PAGGI, L., CASTAGNOLO, L., DELLA SETA, G. & MINERVINI, R. ( 1975). Ricerche sperimentali sul ciclo biologico di Phyllodistomum elongatum Nybelin, 1926 (Digenea: Gorgoderidae Looss, 1901). Parassitologia 17, 95–101.Google Scholar
ORLOVSKAYA, O. M., ATRASHKEVICH, G. I. & BARSHENE, Y. V. ( 1995). Phyllodistomum pungitii sp. n. (Trematoda: Gorgoderidae) from the fish Pungitius pungitius of Chukotka. Parazitologya 29, 532–537. (In Russian.)Google Scholar
PETKEVIČIŪTĖ, R. & STANEVIČIŪTĖ, G. ( 1999). Karyotypic characterization of Apatemon gracilis. Journal of Helminthology 73, 73–77.Google Scholar
PETKEVIČIŪTĖ, R., STANEVIČIŪTĖ, G. & MOLLOY, D. P. ( 2003). Chromosome analysis of Phyllodistomum folium (Trematoda, Gorgoderidae) infecting three European populations of zebra mussels. Parasitology Research 90, 377–382.CrossRefGoogle Scholar
PIGULEWSKY, S. W. ( 1953). Family Gorgoderidae Looss, 1901. In Trematodes of Animals and Man, Vol. 8 (ed. Skryabin, K. I.), pp. 253–615. Izdatelstvo Academii Nauk SSSR, Moscow. (In Russian.)
SINITSIN, D. T. ( 1905). Data on the Natural History of Trematodes. Distomes of Fishes and Frogs in the Vicinity of Warsaw. Warsaw University, Warsaw. (In Russian.)
STUNCÈNAS, V., CRYAN, J. R. & MOLLOY, D. P. ( 2004). Comparison of rDNA sequences from colchicine treated and untreated sporocysts of Phyllodistomum folium and Bucephalus polymorphus (Digenea). Parasitology International 53, 223–228.CrossRefGoogle Scholar
TKACH, V., PAWLOWSKI, J. & MARIAUX, J. ( 2000). Phylogenetic analysis of the suborder Plagiorchiata (Platyhelminthes, Digenea) based on partial 1srDNA sequences. International Journal for Parasitology 30, 83–93.CrossRefGoogle Scholar
ZDUN, V. I., KISELIENĖ, V. K., KARATAYEV, A. Y. & MAKAROVA, G. E. ( 1994). Parasites. In Freshwater Zebra Mussel Dreissena polymorpha (Pall.) (Bivalvia, Dreissenidae). Systematics, Ecology, Practical Meaning (ed. Starobogatov, J. I.), pp. 196–205. Nauka, Moscow. (In Russian.)
ZHOKHOV, A. E. ( 1987). New data on the developmental cycle and biology of the trematode Phyllodistomum elongatum (Fasciolata, Gorgoderidae). Parazitologya 21, 134–139. (In Russian.)Google Scholar