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Cuticle production and ecdysis in larval marine ascaridoid nematodes in vitro

Published online by Cambridge University Press:  06 April 2009

J. G. Mercer ,
A. E. Munn ,
J. W. Smith  and
H. H. Rees
J. G. Mercer
Affiliation:
Department of Biochemistry, University of Liverpool, P. O. Box 147, Liverpool
A. E. Munn
Affiliation:
Department of Biochemistry, University of Liverpool, P. O. Box 147, Liverpool
J. W. Smith
Affiliation:
DAFS Marine Laboratory, Aberdeen
H. H. Rees
Affiliation:
Department of Biochemistry, University of Liverpool, P. O. Box 147, Liverpool
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Summary

Infective 3rd-stage larvae of three species of marine ascaridoid nematodes, Pseudoterranova (= Phocanema = Porrocaecum = Terranova) decipiens, Anisakis simplex and Phocascaris/Contracaecum sp. were cultured in vitro in 0·9% saline or Medium 199 at 37°C beneath an atmosphere of either air or 95% air:5% CO2 All three species responded to culture at 37°C by producing a new cuticle. The majority of P. decipiens completed ecdysis under all the culture conditions employed. Ecdysis in A. simplex was stimulated by a high concentration of CO2; this effect was particularly noticeable in saline cultures. Both A. simplex and Phocascaris/Contracaecum sp. developed more effectively in a nutrient medium than in saline.

Type
Research Article
Information
Parasitology , Volume 92 , Issue 3 , June 1986 , pp. 711 - 720
Copyright
Copyright © Cambridge University Press 1986

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References

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