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Cultivation of Blastocrithidia triatomae (Trypanosomatidae) on a cell line of its host Triatoma infestans (Reduviidae) *

Published online by Cambridge University Press:  06 April 2009

Dagmar Reduth ,
G. A. Schaub  and
Mary Pudney
Dagmar Reduth
Affiliation:
Institut für Biologie I (Zoologie), Albert-Ludwigs-Universität, Albertstrasse 21a, D-7800 Freiburg, Federal Republic of Germany
G. A. Schaub
Affiliation:
Institut für Biologie I (Zoologie), Albert-Ludwigs-Universität, Albertstrasse 21a, D-7800 Freiburg, Federal Republic of Germany
Mary Pudney
Affiliation:
Department of Entomology, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, U.K.
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Summary

Blastocrithidia triatomae parasitizes vectors of Chagas' disease and is very difficult to cultivate in conventional media. However, co-cultivation with a cell line of its host Triatoma infestans (TI-32; in Schneider's Drosophila medium supplemented with 20% foetal calf serum and 10% tryptose phosphate broth) led to vigorous growth at 24 or 28 °C without an adaptation phase. More than 60 primary cultures were initiated successfully without any failures. Subcultures could be started immediately at weekly intervals. The doubling time was similar in both the primary cultures (41 h) and the 33rd subculture (39 h). The importance of the reduviid cells for B. triatomae became clear after removal of the insect cells, when multiplication of epimastigotes stopped and mainly cysts were formed. Cysts produced in vitro were infective for reduviids. Scanning electron microscopy showed that B. triatomae attached to the host cells, inserted its flagellum into them and destroyed them.

Keywords

Blastocrithidia triatomaeChagas' diseaseTriatoma infestans
Type
Research Article
Information
Parasitology , Volume 98 , Issue 3 , June 1989 , pp. 387 - 393
Copyright
Copyright © Cambridge University Press 1989

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