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Cross-reactivity of the 31 kDa antigen of Angiostrongylus cantonensis – Dealing with the immunodiagnosis of meningoencephalitis

Published online by Cambridge University Press:  21 November 2016

ALESSANDRA L. MORASSUTTI ,
LISA N. RASCOE ,
SUKWAN HANDALI ,
ALEXANDRE J. DA SILVA ,
PATRICIA P. WILKINS  and
CARLOS GRAEFF-TEIXEIRA
ALESSANDRA L. MORASSUTTI*
Affiliation:
Laboratório de Biologia Parasitária da Faculdade de Biociências e Laboratório de Parasitologia Molecular do Instituto de Pesquisas Biomédicas da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av Ipiranga 6690, 90690-900 Porto Alegre RS, Brasil
LISA N. RASCOE
Affiliation:
Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, Georgia 30329, USA
SUKWAN HANDALI
Affiliation:
Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, Georgia 30329, USA
ALEXANDRE J. DA SILVA
Affiliation:
U.S. Food & Drug Administration, Division of Virulence Assessment, Center for Foods Safety and Applied Nutrition, Office of Applied Research and Safety Assessment, Silver Spring, Maryland, USA
PATRICIA P. WILKINS
Affiliation:
Division of Parasitic Diseases and Malaria, Center for Global Health, Centers for Disease Control and Prevention, 1600 Clifton Road NE, Atlanta, Georgia 30329, USA
CARLOS GRAEFF-TEIXEIRA
Affiliation:
Laboratório de Biologia Parasitária da Faculdade de Biociências e Laboratório de Parasitologia Molecular do Instituto de Pesquisas Biomédicas da Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Av Ipiranga 6690, 90690-900 Porto Alegre RS, Brasil
*
*Corresponding author: Instituto de Pesquisas Biomédicas da PUCRS, Avenida Ipiranga 6690, 2 andar, Sala 20, CEP: 90690-900 Porto Alegre RS, Brazil. E-mail: [email protected]
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Summary

The primary causative agent of eosinophilic meningoencephalitis (EoM) in endemic regions is the nematode Angiostrongylus cantonensis. The occurrence of EoM was previously restricted to countries in Southeast Asia and the Pacific Islands; however, more recently, it has been reported from other regions, including Brazil. The commonly used diagnosis is detection of specific antibody reactivity to the 31 kDa antigen, which is derived from female worm somatic extracts. Here we report the occurrence of cross-reactivity to this antigen in sera from other parasitic infections, especially those that may cause EoM, such as gnathostomiasis, toxocariasis, hydatidosis and strongyloidiasis. We also demonstrated that the cross-reactivity, in part, is dependent of the concentration of antigen used in Western blot assays. We discuss the importance of these findings on the interpretation of this test.

Keywords

Angiostrongyliasis diagnosis31 kDa antigencross-reactivityeosinophilic meningoencephalitis
Type
Research Article
Information
Parasitology , Volume 144 , Issue 4 , April 2017 , pp. 459 - 463
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Copyright
Copyright © Cambridge University Press 2016 

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