Hostname: page-component-586b7cd67f-dlnhk Total loading time: 0 Render date: 2024-11-24T12:39:47.873Z Has data issue: false hasContentIssue false
  • English
  • Français

Corynebacterium parvum as an adjuvant for Trypanosoma cruzi epimastigote vaccines: a comparison with saponin and Bordetella pertussis

Published online by Cambridge University Press:  06 April 2009

R. Bomford  and
N. McHardy
R. Bomford
Affiliation:
Department of Experimental Immunobiology and Department of Protozoology, The Wellcome Research Laboratories, Beckenham, Kent BR3 3B2
N. McHardy
Affiliation:
Department of Experimental Immunobiology and Department of Protozoology, The Wellcome Research Laboratories, Beckenham, Kent BR3 3B2
Get access Rights & Permissions [Opens in a new window]

Summary

The effect was compared in CBA mice of adding Corynebacterium parvum, saponin, and Bordetella pertussis to living or killed Trypanosoma cruzi (Y strain) epimastigote vaccines on the induction of protective immunity against subcutaneous (s.c.) challenge with blood trypomastigotes. The addition of C. parvum to a low dose of T. cruzi vaccine, which alone was non-protective, generated a greater degree of protection than did saponin or B. pertussis. C. parvum alone increased resistance to infection to a variable and usually weak extent. The addition of C. parvum to larger doses of T. cruzi vaccine, which were themselves sufficient to elicit some degree of protection, improved resistance when the challenge was given 1 or 12 weeks after immunization, but lowered it at 3 weeks. It is concluded that the comparative efficacy of adjuvants for T. cruzi vaccines needs to be assessed on 3 parameters: (1) the dose of antigen, (2) the dose of adjuvant and (3) the time interval between immunization and challenge.

Type
Research Article
Information
Parasitology , Volume 78 , Issue 1 , February 1979 , pp. 77 - 87
Copyright
Copyright © Cambridge University Press 1979

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Bomford, R. (1975). Active specific immunotherapy of mouse methylcholanthrene induced tumours with Corynebacterium parvum and irradiated tumour cells. British Journal of Cancer 32, 551–7.CrossRefGoogle ScholarPubMed
Boné, G. J. & Parent, G. (1963). Stearic acid, an essential growth factor for Trypanosoma cruzi. Journal of General Microbiology 31, 261–6.CrossRefGoogle ScholarPubMed
Brener, Z. & Cardoso, J. E. (1976). Nonspecific resistance against Trypanosoma cruzi enhanced by Corynebacterium parvum. Journal of Parasitology 62, 645–6.CrossRefGoogle ScholarPubMed
Clark, I. A., Cox, F. E. G. & Allison, A. C. (1977). Protection of mice against Babesia spp. and Plasmodium spp. with killed Corynebacterium parvum. Parasitology 74, 9–18.CrossRefGoogle ScholarPubMed
Dresser, D. W., Wortis, H. H. & Anderson, H. R. (1970). The effect of pertussis vaccine on the immune response of mice to sheep red blood cells. Clinical and Experimental Immunology 7, 817–31.Google ScholarPubMed
Garnham, P. C. C. & Humphrey, J. H. (1969). Problems in leishmaniasis related to immunology. Current Topics in Microbiology and Immunology 48, 29–42.Google ScholarPubMed
Goble, F. C. (1970). South American trypanosomes. In Immunity to Parasitic Animals, vol. 2 (ed. Jackson, G. J., Herman, R. and Singer, I.), pp. 597–689. New York: Appleton-Century-Crofts.Google Scholar
Gonzalez-Cappa, S. M., Schmunis, G. A., Traversa, O. C., Yanovsky, J. F. & Parodi, A. S. (1968). Complement-fixation tests, skin tests, and experimental immunization with antigens of Trypanosoma cruzi prepared under pressure. American Journal of Tropical Medicine and Hygiene 17, 709–15.CrossRefGoogle ScholarPubMed
Hanson, W. L., Chien, J. & Chapman, W. L. (1973). Partial resistance produced in mice by inoculation with irradiated Trypanosoma cruzi from cell culture. Journal of Protozoology 20, 511.Google Scholar
Howard, J. G., Scott, M. T. & Christie, G. H. (1973). Cellular mechanisms underlying the adjuvant activity of Corynebacterium parvum: interactions of activated macrophages with T and B lymphocytes. In Immunopotentiation Ciba Fdn Symp. 18 (ed. Wolstenholme, J. W. and Knight, J.), pp. 101–20. Amsterdam: Associated Scientific Publishers.Google Scholar
Johnson, P., Neal, R. A. & Gall, D. (1963). Protective effect of killed trypanosome vaccines with incorporated adjuvants. Nature, London 200, 83.CrossRefGoogle ScholarPubMed
Kierszenbaum, F. (1975). Enhancement of resistance and suppression of immunization against experimental Trypanosoma cruzi infection by Corynebacterium parvum. Infection and Immunity 12, 1227–9.CrossRefGoogle ScholarPubMed
Kierszenbaum, F. & Budzko, B. B. (1975). Immunization against experimental Chagas' disease by using culture forms of Trypanosoma cruzi killed with a solution of sodium perchlorate. Infection and Immunity 12, 461–5.CrossRefGoogle ScholarPubMed
Kierszenbaum, F. & Howard, J. G. (1976). Mechanisms of resistance against experimental Trypanosoma cruzi infection: the importance of antibodies and antibody-forming capacity in the Biozzi high and low responder mice. Journal of Immunology 116, 1208–11.CrossRefGoogle ScholarPubMed
Mauel, J. & Behin, R. (1974). Cell-mediated and humoral immunity to protozoan infections (with special reference to leishmaniasis). Transplantation Reviews 19, 121–46.Google Scholar
McHardy, N. (1977 a). Immunization of mice against Trypanosoma cruzi. The effect of size of dose and route of injection of immunizing and challenging inocula. Tropenmedizin und Parasitologie 28, 11–16.Google ScholarPubMed
McHardy, N. (1977 b). Passive immunization of mice against Trypanosoma cruzi using convalescent mouse serum. Tropenmedizin und Parasitologie 28, 195–201.Google ScholarPubMed
Menezes, H. (1965). O omprego de adjuvantes na vacinacao do camundongos con Trypanosoma cruzi. Revista brasileira de Medicina 22, 536–8.Google Scholar
Milas, L. & Scott, M. T. (1977). Antitumor activity of Corynebacterium parvum. Advances in Cancer Research 26, (in the Press).Google Scholar
Neal, R. A. & Johnson, P. (1977). Immunization against Trypanosoma cruzi using killed antigens and with saponin as adjuvant. Acta Tropica 34, 87–96.Google ScholarPubMed
Nussenzweig, R. S. (1967). Increased non-specific resistance to malaria produced by administration of killed Corynebacterium parvum. Experimental Parasitology 21, 224–31.CrossRefGoogle Scholar
Scott, M. T. (1975). Potentiation of the tumor-specific immune response by Corynebacterium parvum. Journal of the National Cancer Institute 55, 65–72.CrossRefGoogle ScholarPubMed
Seneca, H., Peer, P. & Hampar, B. (1966). Active immunization of mice with chagastoxin, a polysaccharide prepared from Trypanosoma cruzi. Nature, London 209, 309–10.CrossRefGoogle Scholar
Swartzberg, J. E., Krahenbuhl, J. L. & Remington, J. S. (1975). Dichotomy between macrophage activation and degree of protection against Listeria monocytogenes and Toxoplasma condi in mice stimulated with Corynebacterium parvum. Infection and Immunity 12, 1037–43.CrossRefGoogle Scholar
Tuttle, R. L. & North, R. J. (1976). Mechanisms of antitumor action of Corynebacterium parvum: replicating short-lived T cells as the mediators of potentiated tumor-specific immunity. Journal of the Reticuloendothelial Society 20, 209–16.Google ScholarPubMed