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Coproantigen detection in rats experimentally infected with Strongyloides ratti

Published online by Cambridge University Press:  06 April 2009

C. Nageswaran ,
P. S. Craig  and
E. Devaney
C. Nageswaran
Affiliation:
Department of Parasitology, Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA
P. S. Craig
Affiliation:
Department of Biological Sciences, University of Salford, Salford M5 4WT
E. Devaney
Affiliation:
Department of Veterinary Parasitology, University of Glasgow, Glasgow G61 1QH
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Summary

The possibility of coproantigen detection in the diagnosis of strongyloidiasis was investigated in rats experimentally infected with Strongyloides ratti. A capture ELISA was developed using affinity-purified polyclonal antibodies raised in rabbits against adult and filariform larval somatic antigens. The first detection of coproantigen coincided with patency and the coproantigen level remained elevated even when the parasite count was low. Following challenge infection, when examination of stool for larvae or ova was negative, coproantigen was detected by anti-adult somatic capture ELISA. Despite the use of antisera raised against crude worm extracts, the test was specific showing only low cross-reactivity with Necator americanus and Syphacia muris.

Keywords

Strongyloides ratticoproantigen detectionhumoral responseexperimental infection
Type
Research Article
Information
Parasitology , Volume 108 , Issue 3 , April 1994 , pp. 335 - 342
Copyright
Copyright © Cambridge University Press 1994

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