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Comparison of protein composition between schistosomula of Schistosoma japonicum and S. mansoni

Published online by Cambridge University Press:  06 April 2009

S. Maeda ,
Y. Irie  and
K. Yasuraoka
S. Maeda
Affiliation:
Department of Medical Biology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki-ken 305, Japan
Y. Irie
Affiliation:
Department of Medical Biology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki-ken 305, Japan
K. Yasuraoka
Affiliation:
Department of Medical Biology, Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki-ken 305, Japan
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Summary

SDS-PAGE analysis revealed that the protein composition of mechanically transformed schistosomula (ms) of a Japanese strain of Schistosoma japonicum was essentially similar to that of a Philippine strain of S. japonicum. However, the protein components of S. mansoni ms were remarkably different from those of S. japonicum. Specific proteins of Mr 60–65.and 30 kDa were found in ms of S. japonicum and S. mansoni, respectively. In skin-penetrated schistosomula (ss) of the two species, a common protein of 67 kDa was detected. Using two-dimensional electrophoresis, strain-specific polypeptides (9 in a Japanese and 11 in a Philippine strain) were identified in ms of S. japonicum. Mechanically transformed and skin-penetrated schistosomula of S. japonicum (Japanese) had, respectively, 7 and 10 specific proteins. Four and 1 specific polypeptides were also detected in two-dimensional profiles of ms and ss of S. mansoni, respectively.

Type
Research Article
Information
Parasitology , Volume 89 , Issue 3 , December 1984 , pp. 453 - 459
Copyright
Copyright © Cambridge University Press 1984

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