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Comparative analysis of two fatty acid binding proteins from Fasciola gigantica

Published online by Cambridge University Press:  16 June 2010

SUPATRA CHUNCHOB ,
RUDI GRAMS ,
VITHOON VIYANANT ,
PETER M. SMOOKER  and
SUKSIRI VICHASRI-GRAMS
SUPATRA CHUNCHOB
Affiliation:
Department of Biology, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand
RUDI GRAMS
Affiliation:
Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Phaholyothin Rd., Klongluang, Pathumthani, 12121, Thailand
VITHOON VIYANANT
Affiliation:
Graduate Program in Biomedical Sciences, Faculty of Allied Health Sciences, Thammasat University, Phaholyothin Rd., Klongluang, Pathumthani, 12121, Thailand
PETER M. SMOOKER
Affiliation:
School of Applied Sciences, RMIT University, BundooraVIC 3032, Australia
SUKSIRI VICHASRI-GRAMS*
Affiliation:
Department of Biology, Faculty of Science, Mahidol University, Rama VI Rd., Bangkok, 10400, Thailand
*
*Corresponding author: Department of Biology, Faculty of Science, Mahidol University, 272 Rama VI Road, Ratchathewi, Bangkok 10400, Thailand. Tel: +662 201 5480. Fax: +662 354 7161. E-mail: [email protected]
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Summary

Fatty acid binding proteins are considered to be promising vaccine candidates against trematodiasis. In order to provide additional information about their function in Fasciola gigantica we performed a comparative analysis of FgFABP1 and FgFABP3, two isoforms with quite different isoelectric points of 4·9 and 9·9 and 67% sequence identity. Both are expressed in the juvenile and adult parasite but differ in their tissue-specific distribution. In addition, the sequence of FABP3 is identical in F. hepatica and F. gigantica indicating the protein's functional importance in this genus. Immune sera produced against soluble recombinant FgFABPs reacted with 14 kDa antigens in crude worm, soluble egg, cirrus sac extracts, and excretion/secretion product. Both FgFABPs were located in the parenchyma of the parasite but in addition, FgFABP1 was abundant in testes and spermatozoa while FgFABP3 was abundant in vitelline cells, eggs, and caecal epithelium. Mass spectrometry identified FgFABP1 and FgFABP3 in the ES product whereas only FgFABP3 was identified in egg extract. Serum samples of an experimentally infected rabbit reacted from week 6 post-infection with FgFABP3 and from week 12 with FgFABP1 while sera of infected sheep were not reactive. The results suggest differences in the biological functions of these 2 isoforms and differences in the host/parasite interaction that should be considered for their potential as vaccines against fascioliasis.

Keywords

Fasciola giganticafatty acid binding proteintissue-specificity2DE proteomics
Type
Research Article
Information
Parasitology , Volume 137 , Issue 12 , October 2010 , pp. 1805 - 1817
Copyright
Copyright © Cambridge University Press 2010

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