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Codon usage in Cryptosporidium parvum differs from that in other Eimeriorina

Published online by Cambridge University Press:  06 April 2009

S. Char ,
P. Kelly ,
A. Naeem  and
M. J. G. Farthing
S. Char*
Affiliation:
Digestive Diseases Research Centre, The Medical College of St Bartholomew's Hospital, London EC1M 6BQ
P. Kelly
Affiliation:
Digestive Diseases Research Centre, The Medical College of St Bartholomew's Hospital, London EC1M 6BQ
A. Naeem
Affiliation:
Digestive Diseases Research Centre, The Medical College of St Bartholomew's Hospital, London EC1M 6BQ
M. J. G. Farthing
Affiliation:
Digestive Diseases Research Centre, The Medical College of St Bartholomew's Hospital, London EC1M 6BQ
*
* Corresponding author.
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Summary

Codon usage of Crytosporidium parvum was compared with those of other Eimeriorina Toxoplasma gondii and Eimeria tenella and revealed a biased use of synonymous codons with a preference for NNU (40·0%) and NNA (33·4%). There was no close resemblance of the codon usage of C. parvum to T. gondii (correlation coefficient, r = 0·14) or E. tenella (r = 0·14) but it was similar to Entamoeba histolytica (r = 0·75) and Plasmodium falciparum (r = 0·5). Analysis of the codon usage in homologous gene sequences (actin, β-tubulin) also failed to reveal a close relationship between C. parvum and T. gondii or E. tenella. The low usage codons in C. parvum were most frequently used codons in T. gondii and E. tenella. These observations are consistent with 18S rRNA sequence analysis which shows no close relationship of Cryptosporidium with other Eimeriorina (Sarcocystis, Toxoplasma and Eimeria) and questions the validity of the current classification of C. parvum.

Keywords

coccidiacodon usageCryptosporidiumEimeriorina
Type
Research Article
Information
Parasitology , Volume 112 , Issue 4 , April 1996 , pp. 357 - 362
Copyright
Copyright © Cambridge University Press 1996

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