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Cloning of a repetitive DNA from the rickettsia-like organisms of tsetse flies (Glossina spp.)

Published online by Cambridge University Press:  06 April 2009

S. C. Welburn  and
W. C. Gibson
S. C. Welburn
Affiliation:
Tsetse Research Laboratory, ODA/University of Bristol, Langford, Bristol BS18 7DU
W. C. Gibson
Affiliation:
Department of Pathology, University of Bristol, Langford, Bristol BS18 7DU
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Summary

Three DNA fragments from the genome of the rickettsia-like organism (RLO) symbiotic in tsetse flies (Glossina spp.) have been cloned. One of these fragments represents a family of highly conserved repeats, which occurs in RLO from all species of tsetse examined and has been amplified in the original RLO culture from which the DNA is cloned. This fragment serves as a highly sensitive and specific probe for the detection of RLO in tsetse midguts. As few as 30 organisms were unequivocally identified by dot blot hybridization of homogenized midgut preparations. Since the presence of RLO within tsetse midguts is associated with susceptibility to trypanosome infection, this technique provides a rapid and reliable method of assessing the potential susceptibility of a tsetse population to Trypanosoma brucei s.l. and T. congolense infections.

Keywords

DNA proberickettsiatsetsetrypanosomewolbachia
Type
Research Article
Information
Parasitology , Volume 98 , Issue 1 , February 1989 , pp. 81 - 84
Copyright
Copyright © Cambridge University Press 1989

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References

Feinberg, A. P. & Vogelstein, B. (1983). A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Analytical Biochemistry 132, 6–13.CrossRefGoogle ScholarPubMed
Giminez, D. F. (1964). Staining rickettsiae in yolk-culture. Stain Technology 39, 135–40.CrossRefGoogle Scholar
Gibson, W. C., Dukes, P. & Gashumba, J. K. (1988). Species-specific DNA probes for the identification of African trypanosomes in tsetse flies. Parasitology 97, 63–73.CrossRefGoogle ScholarPubMed
Hayes, S. F. & Burgdorfer, W. B. (1981). Ultrastructural comparisons of wolbachia-like symbiotes of ticks (Acari: Ixodidae). In Rickettsiae and Rickettsial Diseases (ed. Burgdorfer, W. and Anacker, R. L.) London: Academic Press.Google Scholar
Maudlin, I. & Ellis, D. S. (1985). Association between intracellular rickettsia-like infections of midgut cells and susceptibility to trypanosome infection in Glossina species. Zeitschrift für Parasitenkunde 71, 683–7.CrossRefGoogle Scholar
Maudlin, I., Dukes, P., Luckins, A. G. & Hudson, K. M. (1986). Extrachromosomal inheritance of susceptibility to trypanosome infection in tsetse flies. II. Susceptibility of selected lines of Glossina morsitans morsitans to different stocks and species of trypanosomes. Annals of Tropical Medicine and Parasitology 80, 97–105.CrossRefGoogle Scholar
Reinhardt, C., Steiger, R. & Hecker, H. (1972). Ultrastructural study of the midgut mycetomebacteroids of the tsetse flies Glossina morsitans, G. fuscipes, and G. brevipalpis (Diptera, Brachycera). Acta Tropica 29, 280–8.Google Scholar
Southern, E. (1975). Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98, 503–17.CrossRefGoogle ScholarPubMed
Welburn, S. C., Maudlin, I. & Ellis, D. S. (1987). In vitro cultivation of rickettsia-like-organisms from Glossina spp. Annals of Tropical Medicine and Parasitology 81, 331–5.CrossRefGoogle ScholarPubMed
Young, C. J. & Godfrey, D. G. (1983). Enzyme polymorphism and the distribution of Trypanosoma congolense isolates. Annals of Tropical Medicine and Parasitology 77, 467–81.CrossRefGoogle ScholarPubMed