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Characterization of the exposed carbohydrates on the surface membrane of adult Schistosoma mansoni by analysis of lectin binding

Published online by Cambridge University Press:  06 April 2009

A. J. G. Simpson  and
S. R. Smithers
A. J. G. Simpson
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
S. R. Smithers
Affiliation:
Division of Parasitology, National Institute for Medical Research, Mill Hill, London NW7 1AA
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Summary

The surface architecture of adult male Schistosoma mansoni was explored using a range of lectins with differing carbohydrate specificities. Highest specific binding was achieved with concanavalin A and the agglutinin of molecular weight 60000 from Ricinus communis; the binding of wheat germ agglutinin was mostly non-specific. Small amounts of peanut agglutinin and soybean agglutinin binding were observed and the binding of these lectins was increased by pre-treating the parasite with neuraminidase. The fucose binding protein of Lotus tetragonolobus failed to bind. These results indicate that mannose and/or glucose, galactose, N-acetyl-glucosamine, N-acetyl-galactosamine and sialic acid are exposed on the surface of the adult male schistosome.

Type
Research Article
Information
Parasitology , Volume 81 , Issue 1 , August 1980 , pp. 1 - 15
Copyright
Copyright © Cambridge University Press 1980

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References

REFERENCE

Bennett, J. L., & Seed, J. L., (1977). Characterization and isolation of concanavalin A binding sites from the epidermis of S. mansoni. Journal of Parasitology 63, 250–8.CrossRefGoogle ScholarPubMed
Bouchard, P., Moroux, Y., Tixier, R., Privat, J-P., & Monsigny, M., (1976). An improved method for purification of wheat germ agglutinin (lectin) by affinity chromatography. Biochemie 58, 1247–53.CrossRefGoogle ScholarPubMed
Goldring, O. L., Clegg, J. A., Smithers, S. R., & Terry, R. J., (1976). Acquisition of human blood group antigens by Schistosoma mansoni. Clinical and Experimental Immunology 26, 181–7.Google ScholarPubMed
Hughes, R. C., (1976). Membrane glycoproteins. A Review of Structure and Function. London: Butterworth.Google Scholar
Hunter, W. M., & Greenwood, F. C., (1962). Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature, London 194, 495–6.CrossRefGoogle ScholarPubMed
Kornfeld, S., & Kornfeld, R., (1969). Solubilization and partial characterization of a phytohaemagglutinin receptor site from human erythrocytes. Proceedings of the National Academy of Science, USA 63, 1439–46.CrossRefGoogle ScholarPubMed
Kornfeld, R., & Kornfeld, S., (1970). The structure of a phytohaemagglutinin receptor site from human erythrocytes. Journal of Biological Chemistry 245, 2536–45.CrossRefGoogle ScholarPubMed
Lis, H., Sela, B-A., Sachs, L., & Sharon, N., (1970). Specific inhibition by N-acetyl-D-galactosamine of the interaction between soybean agglutinin and animal cell surfaces. Biochimica et biophysica acta 211, 582–5.CrossRefGoogle ScholarPubMed
Lotan, R., Skutelsky, E., Danon, D., & Shabon, N., (1975). The purification, composition and specificity of the anti-T lectin from peanut (Arachis hypogaea). Journal of Biological Chemistry 250, 8518–23.CrossRefGoogle ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Fabb, A. L., & Randall, R. J., (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
McLaren, D. J., (1974). The anterior glands of adult Necator americanus (Nematoda: Strongyloidea). I. Ultrastructural studies. International Journal for Parasitology 4, 25–37.CrossRefGoogle ScholarPubMed
Mclaren, D. J., Hockley, D. J., Goldbing, O. L., & Hammond, B. J., (1978). A freeze fracture study of the developing tegumental outer membrane of Schistosoma mansoni. Parasitology 76, 327–48.CrossRefGoogle ScholarPubMed
Morris, G. P., & Threadgold, L. T., (1968). Ultrastructure of the tegument of adult Schistosoma mansoni. Journal of Parasitology 54, 15–27.CrossRefGoogle ScholarPubMed
Murrell, K. D., Taylor, D. W., Vannier, W. E., & Dean, D. A., (1978). Schistosoma mansoni: analysis of surface membrane carbohydrates using lectins. Experimental Parasitology 46, 247–55.CrossRefGoogle ScholarPubMed
Nathenson, S. G., & Cullen, S. E., (1974). Biochemical properties and immunochemicalgenetic relationships of mouse H2-alloantigens. Biochimica et biophysica acta 344, 1–25.CrossRefGoogle Scholar
Nicolson, G. L., Blaustein, J., & Etzler, M. E., (1974). Characterization of two plant lectins from Ricinus communis and their quantitative interaction with a murine lymphoma Biochemistry 13, 196–204.CrossRefGoogle ScholarPubMed
Noonan, K. D., & Burger, M. M., (1973). Binding of [3H] concanavalin A to normal and transformed cells. Journal of Biological Chemistry 248, 4286–92.CrossRefGoogle ScholarPubMed
Pereira, M. E. A., & Kabat, E. A., (1974 a). Immunochemical studies on the specificity of soybean agglutinin. Carbohydrate Research 37, 89–102.CrossRefGoogle ScholarPubMed
Pereira, M. E. A., & Kabat, E. A., (1974 b). Specificity of purified hemagglutinin (lectin) from Lotus tetragonolobus. Biochemistry 13, 3184–92.CrossRefGoogle ScholarPubMed
Rumjanek, F. D., Broomfield, K. E., & Smithers, S. R., (1979). Schistosoma mansoni: glycosyl transferase activity and the carbohydrate composition of the tegument. Experimental Parasitology 47, 24–35.CrossRefGoogle ScholarPubMed
Scatchard, G., (1949). The attraction of proteins for small molecules and ions. Annals ofthe New York Academy of Sciences 51, 660–72.CrossRefGoogle Scholar
Smithers, S. R., & Terry, R. J., (1965). Infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of adult worms. Parasitology 55, 695–700.CrossRefGoogle ScholarPubMed
Stein, P. C., & Lumsden, R. D., (1973). Schistosoma mansoni: topochemical features of cercariae, schistosomula and adults. Experimental Parasitology 33, 499–514.CrossRefGoogle ScholarPubMed
Wilson, R. A., & Babnes, P. E., (1977). The formation and turnover of the membranocalyx of the tegument of Schistosoma mansoni. Parasitology 74, 61–71.CrossRefGoogle ScholarPubMed