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Characterization of the effector mechanisms of a transmission-blocking antibody upon differentiation of Plasmodium berghei gametocytes into ookinetes in vitro

Published online by Cambridge University Press:  06 April 2009

G. R. R. Ranawaka ,
A. R. Alejo-Blanco  and
R. E. Sinden
G. R. R. Ranawaka
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Biology, Imperial College, London SW7 2BB
A. R. Alejo-Blanco
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Biology, Imperial College, London SW7 2BB
R. E. Sinden
Affiliation:
Molecular and Cellular Parasitology Research Group, Department of Biology, Imperial College, London SW7 2BB
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Summary

The transmission-blocking monoclonal antibody 13.1, which recognizes the ookinete surface antigen Pbs21 of Plasmodium berghei, and an IgG2a isotype control antibody 26.37 were purified by caprylic acid and ammonium sulphate precipitation. Fab fragments were prepared by papain digestion. IgG but not Fab from antibody 13.1 reduced ookinete formation by P. berghei in culture by as much as 94% at a concentration of 100 μg/ml. There was little difference in antibody efficacy in the range 6·25–400 μg/ml in this assay. The parasite was most sensitive to antibody activity in the first 6–9 h of culture, i.e. the gamete/zygote and early retort stages. Peripheral blood leucocytes (PBL) were essential to achieve maximal inhibition by mAb 13.1 (activity was abrogated totally if PBL were removed). Together the data suggest that one of the mechanisms of action of this antibody is antibody-mediated PBL killing. Phagocytosis of parasites was noted in these experiments in all cultures. We have not attempted in this study to distinguish between Fc-mediated opsonization, as opposed to antibody-dependent cellular cytotoxicity.

Keywords

ookinetetransmission-blocking antibodyphagocytosisleucocytes
Type
Research Article
Information
Parasitology , Volume 109 , Issue 1 , July 1994 , pp. 11 - 17
Copyright
Copyright © Cambridge University Press 1994

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