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Carbon dioxide fixation in trypanosomatids

Published online by Cambridge University Press:  06 April 2009

R. A. Klein ,
D. J. Linstead  and
M. V. Wheeler
R. A. Klein
Affiliation:
Medical Research Council, Biochemical Parasitology Unit, Molteno Institute, University of Cambridge, Downing Street, Cambridge CB2 3EE
D. J. Linstead
Affiliation:
Medical Research Council, Biochemical Parasitology Unit, Molteno Institute, University of Cambridge, Downing Street, Cambridge CB2 3EE
M. V. Wheeler
Affiliation:
Medical Research Council, Biochemical Parasitology Unit, Molteno Institute, University of Cambridge, Downing Street, Cambridge CB2 3EE
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Extract

Fixation of carbon dioxide has been demonstrated for extracts from Crithidia fasciculata, Trypanosoma mega and Trypanosoma brucei brucei bloodstream and culture forms. The enzymes involved in this fixation were found to be ADP-stimulated phosphoenolpyruvate carboxykinase (E.C. 4. 1. 1. 32), ‘malic’ enzyme (E.C. 1. 1. 138–40) and pyruvate carboxylase (E. 0. 6. 4. 1. 1). The subcellular localization of these enzymes has been investigated in all three organisms. Products of short and long term fixation experiments were separated and identified.

The importance of carboxylation reactions is discussed in relation to the maintenance of oxidized and reduced coenzyme levels.

Type
Research Article
Information
Parasitology , Volume 71 , Issue 1 , August 1975 , pp. 93 - 107
Copyright
Copyright © Cambridge University Press 1975

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