Hostname: page-component-586b7cd67f-gb8f7 Total loading time: 0 Render date: 2024-11-26T12:56:27.720Z Has data issue: false hasContentIssue false
  • English
  • Français

Attachment and invasion of Toxoplasma gondii and Neospora caninum to epithelial and fibroblast cell lines in vitro

Published online by Cambridge University Press:  11 July 2005

YING LEI ,
M. DAVEY  and
J. T. ELLIS
YING LEI
Affiliation:
Department of Cell and Molecular Biology, Faculty of Science, University of Technology, Sydney, Gore Hill, NSW 2065, Australia
M. DAVEY
Affiliation:
Department of Cell and Molecular Biology, Faculty of Science, University of Technology, Sydney, Gore Hill, NSW 2065, Australia
J. T. ELLIS
Affiliation:
Department of Cell and Molecular Biology, Faculty of Science, University of Technology, Sydney, Gore Hill, NSW 2065, Australia
Get access Rights & Permissions [Opens in a new window]

Abstract

Attachment and invasion of Toxoplasma gondii and Neospora caninum to a cat and a dog fibroblast cell line and 2 epithelial cell lines (a cat kidney and Vero) were compared in vitro using fluorescence antibody methodology. In addition, trypsin treatment of tachyzoites was used to determine whether protein molecules were essential to the process of invasion. The results show that both T. gondii and N. caninum invaded all 4 cell lines, and that pre-treatment of T. gondii tachyzoites with trypsin caused an increase in the ability of the parasite to invade these host cells. Furthermore T. gondii, in comparison to N. caninum, invaded all 4 cell lines at greater levels. The results here support the conclusion that both T. gondii and N. caninum have the ability to invade a variety of cell types including both dog and cat cells, and questions the utility of Vero cells as an appropriate host cell for in vitro studies on the biology of these taxa.

Keywords

Neospora caninumToxoplasma gondiiattachmentinvasionhost cellimmunofluorescence
Type
Research Article
Information
Parasitology , Volume 131 , Issue 5 , November 2005 , pp. 583 - 590
Copyright
© 2005 Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Buxton, D., Maley, S. W., Wright, S., Thompson, K. M., Rae, A. G. and Innes, E. A. ( 1998). The pathogenesis of experimental neosporosis in pregnant sheep. Journal of Comparative Pathology 118, 267–279.CrossRefGoogle Scholar
Canfield, P. J., Hartley, W. J. and Dubey, J. P. ( 1990). Lesions of toxoplasmosis in Australian marsupials. Journal of Comparative Pathology 103, 159–167.CrossRefGoogle Scholar
Carruthers, V. B., Hakansson, S., Giddings, O. K. and Sibley, L. D. ( 2000). Toxoplasma gondii uses sulphated proteoglycans for substrate and host cell attachment. Infection and Immunity 68, 4005–4011.CrossRefGoogle Scholar
Dobrowolski, J. M. and Sibley, L. D. ( 1996). Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite. Cell 84, 933–939.CrossRefGoogle Scholar
Dowse, T. and Soldati, D. ( 2004). Host cell invasion by the apicomplexans: the significance of microneme protein proteolysis. Current Opinion in Microbiology 7, 388–396.CrossRefGoogle Scholar
Dubey, J. P. ( 1994). Toxoplasmosis. Journal of the American Veterinary Medical Association 205, 1593–1598.Google Scholar
Dubey, J. P. and Lindsay, D. S. ( 1996). A review of Neospora caninum and neosporosis. Veterinary Parasitology 67, 1–59.CrossRefGoogle Scholar
Dubey, J. P., Lindsay, D. S. and Speer, C. A. ( 1998). Structure of Toxoplasma gondii tachyzoites, bradyzoites and sporozoites and biology and development of tissue cysts. Clinical Microbiology Reviews 11, 267–299.Google Scholar
Dubey, J. P., Carpenter, J. L., Speer, C. A., Topper, M. J. and Uggla, A. ( 1988). Newly recognized fatal protozoan disease of dogs. Journal of the American Veterinary Medical Association 192, 1269–1285.Google Scholar
Frenkel, J. K. ( 1988). Pathophysiology of toxoplasmosis. Parasitology Today 4, 273–278.CrossRefGoogle Scholar
Grimwood, J. and Smith, J. E. ( 1992). Toxoplasma gondii: the role of a 30 kDa surface protein in host cell invasion. Experimantal Parasitology 74, 106–111.CrossRefGoogle Scholar
Grimwood, J. and Smith, J. E. ( 1996). Toxoplasma gondii: the role of parasite surface and secreted proteins in host cell invasion. International Journal for Parasitology 26, 169–173.CrossRefGoogle Scholar
Hall, S., Ryan, K. A. and Buxton, D. ( 2001). The epidemiology of Toxoplasma infection. In Toxoplasmosis: a Comprehensive Clinical Guide (ed. Joynson, D. H. and Wreghitt, T. J.), pp. 58–124. Cambridge University Press, Cambridge, UK.CrossRef
Hartley, W. J. ( 1966). A review of the epidemiology of toxoplasmosis. Medical Journal of Australia 1, 232–236.Google Scholar
Hartley, W. J. and Dubey, J. P. ( 1991). Fatal toxoplasmosis in some native Australian birds. Journal of Veterinary Diagnostic Investigation 3, 167–169.CrossRefGoogle Scholar
Hemphill, A. ( 1999). The host-parasite relationship in neosporosis. Advances in Parasitology 43, 47–104.CrossRefGoogle Scholar
Hemphill, A., Gottstein, B. and Kaufmann, H. ( 1996). Adhesion and invasion of bovine endothelial cells by Neospora caninum. Parasitology 112, 183–197.CrossRefGoogle Scholar
Henriques, C. and De Souza, W. ( 2000). Redistribution of plasma-membrane surface molecules during formation of the Leishmania amazonensis-containing parasitophorous vacuole. Parasitology Research 86, 215–225.CrossRefGoogle Scholar
Hughes, H. P. A., Hudson, L. and Fleck, D. G. ( 1986). In vitro culture of Toxoplasma gondii in primary and established cell lines. International Journal for Parasitology 16, 317–322.CrossRefGoogle Scholar
Inskeep, W. 2nd, GardinerR, C. H., Harris, R. K., Dubey, J. P. and Goldston, R. T. ( 1990). Toxoplasmosis in Atlantic bottle-nosed dolphins (Tursiops truncatus). Journal of Wildlife Diseases 26, 377–382.CrossRefGoogle Scholar
Laemmli, U. K. ( 1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227, 680–685.CrossRefGoogle Scholar
Linch, J. G., Botero-Kleiven, S., Arboleda, J. I. and Wahgren, M. ( 2001). A protease inhibitor associated with the surface of Toxoplasma gondii. Molecular and Biochemistry Parasitology 116, 137–145.Google Scholar
Lekutis, C., Ferguson, D. J., Grigg, M. E., Camps, M. and Boothroyd, J. C. ( 2001). Surface antigens of Toxoplasma gondii: variations on a theme. International Journal for Parasitology 31, 1285–1292.CrossRefGoogle Scholar
McAllister, M. M., Dubey, J. P., Lindsay, D. S., Jolley, W. R., Wills, R. A. and McGuire, A. M. ( 1998). Dogs are definitive hosts of Neospora caninum. International Journal for Parasitology 128, 1473–1478.CrossRefGoogle Scholar
Miller, N. L., Frenkel, J. K. and Dubey, J. P. ( 1972). Oral infections with Toxoplasma cysts and oocysts in felines, other mammals, and in birds. Journal of Parasitology 58, 928–937.CrossRefGoogle Scholar
Mineo, J. R. and Kasper, C. A. ( 1994). Attachment of Toxoplasma gondii to host cells involves major surface protein SAG-1 (P30). Experimental Parasitology 79, 11–20.CrossRefGoogle Scholar
Mineo, J. R., McLeod, R., Mack, D., Smith, J., Khan, I. A., Ely, K. H. and Kasper, L. H. ( 1993). Antibodies to Toxoplasma gondii major surface protein (SAG-1, P30) inhibit infection of host cells and are produced in murine intestine after peroral infection. Journal of Immunology 150, 3951–3964.Google Scholar
Naguleswaran, A., Cannes, A., Keller, N., Vonlaufen, N., Björkman, C. and Hemphill, A. ( 2002). Vero cell surface proteglycans interacted with the micromenes protein NCMIC 3 mediates adhesion of Neospora caninum tachyzoites to host cells unlike that in Toxoplasma gondii. International Journal for Parasitology 32, 695–704.CrossRefGoogle Scholar
Ngo, H. M., Yang, M. and Joiner, K. A. ( 2004). Are rhoptries in Apicomplexan parasites secretory granules or secretory lysosomal granules? Molecular Microbiology 52, 1531–1541.Google Scholar
Ortega-Barria, E. and Boothroyd, J. C. ( 1999). A Toxoplasma-like activity specific for sulphated polysaccharides is involved in host cell infection. Journal of Biological Chemistry 274, 1267–1276.CrossRefGoogle Scholar
Robinson, S. A., Smith, J. E. and Miller, P. A. ( 2004). Toxoplasma gondii major surface antigen (SAG-1): in vitro analysis of host cell binding. Parasitology 128, 391–396.CrossRefGoogle Scholar
Schenkaman, S., Diaz, C. and Nussenzweig, V. ( 1991). Attachment of Trypanosoma cruzi trypomastigotes to receptors at restricted cell surface domains. Experimental Parasitology 72, 76–86.CrossRefGoogle Scholar
Sibley, L. D. ( 2004). Intracellular parasite invasion strategies. Science 304, 248–253.CrossRefGoogle Scholar
Sundermann, C. A. and Estridge, B. H. ( 1999). Growth of and competition between Neospora caninum and Toxoplasma gondii in vitro. International Journal for Parasitology 29, 1725–1732.CrossRefGoogle Scholar
Vonlaufen, N., Guetg, N., Naguleswaran, A., Müller, N., Björkman, C., Schares, G., Blumroeder, D., Ellis, J. and Hemphill, A. ( 2004). In vitro induction of Neospora caninum bradyzoites in Vero cells reveals differential antigen expression, localization, and host-cell recognition of tachyzoites and bradyzoites. Infection and Immunity 72, 576–583.CrossRefGoogle Scholar