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Association between circulating antigen and parasite load in a model filarial system, Acanthocheilonema viteae in jirds

Published online by Cambridge University Press:  06 April 2009

W. Harnett ,
M. J. Worms ,
M. Grainger ,
S. D. M. Pyke  and
R. M. E. Parkhouse
W. Harnett
Affiliation:
Divisions of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
M. J. Worms
Affiliation:
Divisions of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
M. Grainger
Affiliation:
Divisions of Parasitology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
S. D. M. Pyke
Affiliation:
Mathematical Biology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
R. M. E. Parkhouse
Affiliation:
Immunology, National Institute for Medical Research, The Ridgeway, Mill Hill, LondonNW7 1AA
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Summary

Jirds (Meriones libycus) were infected with various numbers of Acanthocheilonema viteae L3 stage parasites. During the course of the ensuing 16 weeks, blood samples were collected at 2 weekly intervals and the amount of the major parasite excretory–secretory product (E–S 62) and antibodies directed against it measured. After 16 weeks, animals were sacrificed and the size of the mature worm burden established. In spite of interaction between E–S 62 and host antibody, a statistically significant relationship was found to exist between the amount of E–S 62 present in the bloodstream and the size of the parasite load. It is suggested that the detectable antigen level is more influenced by the size of the worm burden than the presence of antibody and that antibody is only likely to affect adversely antigen measurement in situations where the amount released is relatively low. Examples of this are early in infection and in low-level infections. These ideas are discussed in relation to the development and assessment of serological assays which attempt to predict parasite burden in human filarial infections.

Keywords

Acanthocheilonema viteaeantigencirculating antigenexcretory–secretory antigendiagnosisfilaria
Type
Research Article
Information
Parasitology , Volume 101 , Issue 3 , December 1990 , pp. 435 - 444
Copyright
Copyright © Cambridge University Press 1990

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References

REFERENCES

Atkinson, A. C. (1986). Plots, Transformations and Regression. An Introduction to Graphical Methods of Diagnostic Regression Analysis, vol. 1. Oxford: Oxford Statistical Science Series.Google Scholar
Chamberlain, J. P. (1979). Fluorographic detection of radioactivity in polyacrylamide gels, with the water soluble fluor, sodium salicylate. Analytical Biochemistry 98, 132–5.CrossRefGoogle ScholarPubMed
Das, M. K., Subramanyam, V. R., Ravindran, B. & Pattnaik, N. M. (1987). A study of the antigen, antibody and immune complex levels in Wuchereria bancrofti filariasis with reference to clinical status. Journal of Tropical Medicine and Hygiene 90, 135–41.Google ScholarPubMed
Des Moutis, I., Ouaissi, A., Grzych, J. M., Yarzabal, L., Haque, A. & Capron, A. (1983). Onchocerca volvulus: detection of circulating antigen by monoclonal antibodies in human onchocerciasis. American Journal of Tropical Medicine and Hygiene 32, 533–42.CrossRefGoogle ScholarPubMed
Dissanayake, S., Forsyth, K. P., Ismail, M. M. & Mitchell, G. F. (1984). Detection of circulating antigen in bancroftian filariasis by using a monoclonal antibody. American Journal of Tropical Medicine and Hygiene 33, 1130–40.CrossRefGoogle ScholarPubMed
Ehrenberg, J. P., Tamashiro, W. K. & Scott, A. L. (1987). Dirofilaria immitis: identification and characterization of circulating parasite antigens. Experimental Parasitology 63, 205–14.CrossRefGoogle ScholarPubMed
Forsyth, K. P., Mitchell, G. F. & Copeman, D. B. (1984). Onchocerca gibsoni: increase of circulating egg antigen with chemotherapy in bovines. Experimental Parasitology 58, 41–55.CrossRefGoogle ScholarPubMed
Forsyth, K. P., Spark, R., Kazura, J., Brown, G. V., Peters, P., Heywood, P., Dissanayake, S. & Mitchell, G. F. (1985). A monoclonal antibody-bound immunoradiometric assay for detection of circulating antigen in bancroftian filariasis. Journal of Immunology 134, 1172–7.CrossRefGoogle Scholar
Grauer, G. F., Culman, C. A., Bowman, D. D., Mikagrieve, M. & Grieve, R. B. (1988). Parasite excretory–secretory antigen and antibody to excretory–secretory antigen in body fluids and kidney tissue of Dirofilaria immitis infected dogs. American Journal of Tropical Medicine and Hygiene 39, 380–7.CrossRefGoogle ScholarPubMed
Gualzata, M., Weiss, N. & Heusser, C. H. (1986). Dipetalonema viteae: phosphorylcholine and non-phosphorylcholine antigenic determinants in infective larvae and adult worms. Experimental Parasitology 61, 95–102.CrossRefGoogle ScholarPubMed
Hamilton, R. G., Hussain, R. & Ottesen, E. A. (1984). Immunoradiometric assay for detection of filarial antigens in human serum. Journal of Immunology 133, 2237–42.CrossRefGoogle ScholarPubMed
Harnett, W., Worms, M. J., Kapil, A., Grainger, M. & Parkhouse, R. M. E. (1989). Origin, kinetics of circulation and fate in vivo of the major excretory–secretory product of Acanthocheilonema viteae. Parasitology 99, 229–39.CrossRefGoogle ScholarPubMed
Huijun, Z., Zhenghou, T., Reddy, M. V. R., Harinath, B. C. & Piessens, W. F. (1987). Parasite antigens in sera and urine of patients with bancroftian and brugian filariasis detected by sandwich ELISA with monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 36, 554–60.CrossRefGoogle Scholar
Kendall, M. (1987). Kendall's Advanced Theory of Statistics, vol. 11. 5th Edn.London: Griffin.Google Scholar
Kestens, L., Mangelschots, K., van Marck, E. A. E., Gigase, P. L. & Deelder, A. M. (1988). Schistosoma mansoni: impaired clearance of model immune complexes consisting of circulating anodic antigen and monoclonal IgG1 in infected mice. Parasitology Research 74, 356–62.CrossRefGoogle ScholarPubMed
Kirkwood, B. R. (1988). Essentials of Medical Statistics. Oxford: Blackwell Scientific Publications.Google Scholar
Lal, R. B., Paranjape, R. S., Briles, D. E., Nutman, T. B. & Ottesen, E. A. (1987). Circulating parasite antigen(s) in lymphatic filariasis: use of monoclonal antibodies to phosphorylcholine for immunodiagnosis. Journal of Immunology 138, 3554–60.Google ScholarPubMed
Leon, M. A. & Young, N. M. (1971). Specificity for phosphorylcholine of six murine myeloma proteins reactive with Pneumococcus C polysaccharide and β-lipoprotein. Biochemistry 10, 1424–9.CrossRefGoogle ScholarPubMed
Lucius, R., Kapaun, A. & Diesfeld, H. J. (1987). Dipetalonema viteae infection in three species of rodents: species specific patterns of the antibody response. Parasite Immunology 9, 67–80.CrossRefGoogle ScholarPubMed
Lutsch, C., Cesbron, J. Y., Henry, D., Dessaint, J. P., Wandji, K., Ismail, M. & Capron, A. (1988). Lymphatic filariasis: detection of circulating and urinary antigen and differences in antibody isotypes complexed with circulating antigen between symptomatic and asymptomatic subjects. Clinical and Experimental Immunology 71, 253–60.Google ScholarPubMed
Lutsch, C., Cesbron, J. Y., Zahnek, H. & Capron, A. (1987). Detection of circulating and urinary antigens in Mastomys natalensis experimentally infected with Brugia malayi, Brugia pahangi or itomosoides carinii. Parasitology Research 74, 191–5.CrossRefGoogle ScholarPubMed
Maizels, R. M., Burke, J. & Denham, D. A. (1987). Phosphorylcholine-bearing antigens in filarial nematode parasites: analysis of somatic extracts and in vitro secretions of Brugia malayi and B. pahangi. Parasite Immunology 9, 49–66.CrossRefGoogle ScholarPubMed
Maizels, R. M., Morgan, T. M., Gregory, W. F., Selkirk, M. E., Purnomo, Sukartono & Partono, F. (1988). Circulating antibodies and antigens in Presbytis monkeys infected with the filarial parasite Wuchereria bancrofti. Tropical Medicine and Parasitology 39, 214–20.Google ScholarPubMed
Nash, T. R. (1984). Immune complex size determines the clearance rate of a circulating antigen in schistosome infected mice. American Journal of Tropical Medicine and Hygiene 33, 621–6.CrossRefGoogle ScholarPubMed
Ouaissi, A., Kouemeni, L.-C., Haque, A., Ridel, P.-c., Saint Andre, P. & Capron, A. (1981). Detection of circulating antigens in onchocerciasis. American Journal of Tropical Medicine and Hygiene 30, 1211–18.CrossRefGoogle ScholarPubMed
Parker, C. W. (1976). Radioimmunoassay of Biologically Active Compounds. Eagle Wood Cliffs, New Jersey: Prentice-Hall Inc.Google Scholar
Parkhe, K. A., Ramaprasad, P. & Harinath, B. C. (1988). Stick enzyme-linked immunosorbent assay using the avidin-biotin system for detection of circulating antigen in bancroftian filariasis. Journal of Bioscience 13, 229–33.CrossRefGoogle Scholar
Parkhouse, R. M. E., Philip, M. & Ogilvie, B. M. (1981). Characterization of surface antigens of Trichinella spiralis infective larvae. Parasite Immunology 3, 339–52.CrossRefGoogle ScholarPubMed
Reddy, M. V. R., Malhotra, A. & Harinath, B. C. (1984). Detection of circulating antigen in bancroftian filariasis by sandwich ELISA using filarial serum IgG. Journal of Helminthology 58, 259–62.CrossRefGoogle ScholarPubMed
Schlie-Guzman, M. A. & Rivas-Alcala, A. R. (1989). Antigen detection in onchocerciasis: correlation with worm burden. Tropical Medicine and Parasitology 40, 47–50.Google ScholarPubMed
Sutanto, I., Maizels, R. M. & Denham, D. A. (1985). Surface antigens of a filarial nematode: analysis of adult Brugia pahangi surface components and their use in monoclonal antibody production. Molecular and Biochemical Parasitology 15, 203–14.CrossRefGoogle ScholarPubMed
Weil, G. J. (1988). Brugia malayi: detection of parasite antigen in sera from infected jirds. Experimental Parasitology 67, 54–62.CrossRefGoogle ScholarPubMed
Weil, G. J., Blair, L. S., Ewanciw, D. V. & Malatesta, P. F. (1986 a). Use of parasite antigen detection to monitor the success of drug therapy in Dirofilaria immitis infected dogs. Journal of Parasitology 72, 737–40.CrossRefGoogle ScholarPubMed
Weil, G. J., Jain, D. C., Santhanam, S., Malhotra, A., Kumar, H., Sethumadhaven, K. V. P., Liftis, F. & Ghosh, T. K. (1987). A monoclonal antibody-based enzyme immunoassay for detecting parasite antigenemia in bancroftian filariasis. Journal of Infectious Diseases 156, 350–5.CrossRefGoogle ScholarPubMed
Weil, G. J., Kumar, H., Santhanan, S., Sethumadhavan, K. V. P. & Jain, D. C. (1986 b). Detection of circulating parasite antigen in bancroftian filariasis by counter-immunoelectrophoresis. American Journal of Tropical Medicine and Hygiene 35, 565–70.CrossRefGoogle Scholar
Weil, G. J., Malane, M. S. & Powers, K. G. (1984). Detection of circulating parasite antigens in canine dirofilariasis by counter-immunoelectrophoresis. American Journal of Tropical Medicine and Hygiene 33, 425–30.CrossRefGoogle Scholar
Weil, G. J., Malane, M. S., Powers, K. G. & Blair, L. S. (1985). Monoclonal antibodies to parasite antigens found in the serum of Dirofilaria immitis-infected dogs. Journal of Immunology 134, 1185–91.CrossRefGoogle ScholarPubMed
Weiss, N., Van Den Ende, M.-C., Albiez, E. J., Barbiero, V. K., Forsyth, K. & Prince, A. M. (1986). Detection of serum antibodies and circulating antigens in a chimpanzee experimentally infected with Onchocerca volvulus. Transactions of the Royal Society of Tropical Medicine and Hygiene 80, 587–91.CrossRefGoogle Scholar
Wenger, J. D., Forsyth, K. P. & Kazura, J. W. (1988). Identification of phosphorylcholine epitope-containing antigens in Brugia malayi and relation of serum epitope levels to infection status of jirds with Brugian filariasis. American Journal of Tropical Medicine and Hygiene 38, 133–41.CrossRefGoogle ScholarPubMed
Worms, M. J., Terry, R. J. & Terry, A. (1961). Dipetalonema witei: filarial parasite of the jird, Meriones libycus. 1. Maintenance in the laboratory. Journal of Parasitology 47, 963–70.CrossRefGoogle Scholar