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Antigenic analysis of a variant-specific component of Trypanosoma brucei brucei: localization on the surface coat with labelled specific antibodies

Published online by Cambridge University Press:  06 April 2009

J. Fruit ,
D. Afchain ,
A. Petitprez ,
N. Van Meirvenne ,
D. Le Ray ,
D. Bout  and
A. Capron
J. Fruit
Affiliation:
Services d'immunologie et de Biologie Parasitaire (U.E.R. de Médecine) et de Parasitologie (U.E.R. de Pharmacie), 59000 Lille, France
D. Afchain
Affiliation:
Services d'immunologie et de Biologie Parasitaire (U.E.R. de Médecine) et de Parasitologie (U.E.R. de Pharmacie), 59000 Lille, France
A. Petitprez
Affiliation:
Laboratoire de Microscopie Electronique, Institut Pasteur, 59000 Lille
N. Van Meirvenne
Affiliation:
Laboratoires de Protozoologie et de Sérologie, Institut de Médecine Tropicale, 2000 Antwerpen, Belgium
D. Le Ray
Affiliation:
Laboratoires de Protozoologie et de Sérologie, Institut de Médecine Tropicale, 2000 Antwerpen, Belgium
D. Bout
Affiliation:
Services d'immunologie et de Biologie Parasitaire (U.E.R. de Médecine) et de Parasitologie (U.E.R. de Pharmacie), 59000 Lille, France
A. Capron
Affiliation:
Services d'immunologie et de Biologie Parasitaire (U.E.R. de Médecine) et de Parasitologie (U.E.R. de Pharmacie), 59000 Lille, France
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Extract

The nature of antigens present on the surface coat of Trypanosoma brucei brucei (serotype AnTat–1) trypanosomes has been investigated by light and electron microscopy using indirect immunofluorescence and immunoenzymatic labelling techniques. The use of a monospecific anticomponent V serum has shown that the variant-specific antigen, component V, is the major constituent of the surface coat. Antigens of heterologous serotype, of culture form trypanosomes, of host serum proteins or host red blood cells were not demonstrated on the surface coat.

Type
Research Article
Information
Parasitology , Volume 74 , Issue 2 , April 1977 , pp. 185 - 190
Copyright
Copyright © Cambridge University Press 1977

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References

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