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Antibody is responsible for the passive transfer of immunity to mice from rabbits, rats or mice vaccinated with attenuated Schistosoma japonicum cercariae

Published online by Cambridge University Press:  06 April 2009

N. A. Moloney  and
G. Webbe
N. A. Moloney
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
G. Webbe
Affiliation:
London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT
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Summary

Sera from rabbits, rats and mice multiply-vaccinated with attenuated cercariae of Schistosoma japonicum conferred high levels of resistance against challenge to naive recipient mice (up to 97, 64 and 60% respectively). Vaccinated rabbit and rat sera were given before challenge and vaccinated mouse serum 5 days after challenge. To show that the protective factors in these sera were antibodies, vaccinated rabbit and mouse sera were fractionated by protein A-Sepharose and the fractions precipitated by 50% ammonium sulphate. The protein A-Sepharose binding or non-binding fractions in vaccinated rabbit serum transferred approximately equal levels of significant resistance to mice, suggesting that both the IgG and non-IgG components of vaccinated rabbit serum are protective. The major part of the protective activity in vaccinated mouse serum was transferred to recipients by the protein A-Sepharose binding fraction, i.e. the IgG antibodies. Heat inactivation of sera at 56 °C for 3 h affected the protective capacity of vaccinated rat sera, but not that of vaccinated rabbit or mouse sera.

Keywords

Schistosoma japonicumantibodyimmunity
Type
Research Article
Information
Parasitology , Volume 100 , Issue 2 , April 1990 , pp. 235 - 239
Copyright
Copyright © Cambridge University Press 1990

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