Hostname: page-component-cd9895bd7-mkpzs Total loading time: 0 Render date: 2024-12-26T00:29:44.506Z Has data issue: false hasContentIssue false
  • English
  • Français

Antibody dependent cell-mediated cytotoxicity of Trypanosoma cruzi: the release of tritium-labelled RNA, DNA and protein

Published online by Cambridge University Press:  06 April 2009

C. J. Sanderson ,
Marlene M. Bunn Moreno  and
Angel F. Lopez
C. J. Sanderson
Affiliation:
Instituto de Biofísica and Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal Rio de Janeiro, Cidade Universitária – ZC 32, Rio de Janeiro, RJ 20.000, Brasil
Marlene M. Bunn Moreno
Affiliation:
Instituto de Biofísica and Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal Rio de Janeiro, Cidade Universitária – ZC 32, Rio de Janeiro, RJ 20.000, Brasil
Angel F. Lopez
Affiliation:
Instituto de Biofísica and Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal Rio de Janeiro, Cidade Universitária – ZC 32, Rio de Janeiro, RJ 20.000, Brasil
Get access Rights & Permissions [Opens in a new window]

Summary

The cytotoxicity of normal rat spleen cells to antibody-coated Trypanosoma cruzi epimastigotes has been studied by assaying the release of [3H]-labelled macromolecules from the parasites. The release of thymidine (DNA) is slower than the release of uridine (RNA), suggesting that the nucleus is broken down more slowly than the cytoplasmic membrane. Less than 50% of the leucine (protein) is released when the parasites are lysed, whereas uridine (RNA) is almost totally released. In practical terms these results show that the release of incorporated radioisotope-labelled uridine can be used as a sensitive assay for cytotoxicity of T. cruzi. Cytotoxicity by normal rat spleen cells is antibody dependent and proportional to the logarithm of effector cell number. The lag phase and the rate of RNA release is not altered by centrifuging the parasites and effector cells to enhance contacts between them.

Type
Research Article
Information
Parasitology , Volume 76 , Issue 3 , June 1978 , pp. 299 - 307
Copyright
Copyright © Cambridge University Press 1978

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Abrahamsohn, I. A. & Dias de Silva, W. (1977). Antibody dependent cell-mediated cytotoxicity against Trypanosoma cruzi. Parasitology 75, 317–23.CrossRefGoogle ScholarPubMed
Butterworth, A. E., Sturrock, R. F., Houba, V. & Rees, P. H. (1974). Antibody-dependent cell-mediated damage to schistosomula in vitro. Nature, London 252, 503–5.CrossRefGoogle ScholarPubMed
Franks, D., Kelly, M. & Sanderson, C. J. (1975). Inhibition of xenogeneic cell-mediated cytotoxicity in the rat by antiglobulin serum. International Archives of Allergy and Applied Immunology 48, 621–31.CrossRefGoogle Scholar
Kuhn, R. E. & Vaughn, R. T. (1976). An immunocytotoxic assay for Trypanosoma cruzi. International Journal for Parasitology 6, 129–34.CrossRefGoogle ScholarPubMed
Kuhn, R. E., Vaughn, R. T. & Iannuzzi, N. P. (1974). The in vivo distribution of [51Cr] labelled Trypanosoma cruzi in mice. International Journal for Parasitology 4, 585–8.CrossRefGoogle ScholarPubMed
Mkwananzi, J. B., Franks, D. & Baker, J. R. (1976). Cytotoxicity of antibody-coated trypanosomes by normal human lymphoid cells. Nature, London 249, 403–4.CrossRefGoogle Scholar
Sanderson, C. J. (1976). The mechanism of T-cell mediated cytotoxicity. I. The release of different cell components. Proceedings of the Royal Society of London, B 192, 221–39.Google ScholarPubMed
Sanderson, C. J., Lopez, A. F., & Moreno, Bunn M. M. (1977). Eosinophils and not lymphoid K cells kill Trypanosoma cruzi. Nature, London 268, 340–1.CrossRefGoogle Scholar
Subrahmayam, D., Rao, Y. V. B. G., Mehta, K. & Nelson, D. S. (1976). Serum-dependent adhesion and cytotoxicity of cells to Litomosoides carinii microfilia. Nature, London 260, 529–30.CrossRefGoogle Scholar
Warren, I. G., (1960). Metabolism of Schizotrypanum C. Chagas. I. Effect of culture and substrate concentration on respiratory rate. Journal of Parasitology 46, 529–39.CrossRefGoogle Scholar