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Anthelmintic action of plant cysteine proteinases against the rodent stomach nematode, Protospirura muricola, in vitro and in vivo

Published online by Cambridge University Press:  11 October 2006

G. STEPEK ,
A. E. LOWE ,
D. J. BUTTLE ,
I. R. DUCE  and
J. M. BEHNKE
G. STEPEK
Affiliation:
School of Biology, University of Nottingham NG7 2RD, UK
A. E. LOWE
Affiliation:
School of Biology, University of Nottingham NG7 2RD, UK
D. J. BUTTLE
Affiliation:
Division of Genomic Medicine, University of Sheffield S10 2RX, UK
I. R. DUCE
Affiliation:
School of Biology, University of Nottingham NG7 2RD, UK
J. M. BEHNKE
Affiliation:
School of Biology, University of Nottingham NG7 2RD, UK
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Abstract

Cysteine proteinases from the fruit and latex of plants, including papaya, pineapple and fig, were previously shown to have a rapid detrimental effect, in vitro, against the rodent gastrointestinal nematodes, Heligmosomoides polygyrus (which is found in the anterior small intestine) and Trichuris muris (which resides in the caecum). Proteinases in the crude latex of papaya also showed anthelmintic efficacy against both nematodes in vivo. In this paper, we describe the in vitro and in vivo effects of these plant extracts against the rodent nematode, Protospirura muricola, which is found in the stomach. As in earlier work, all the plant cysteine proteinases examined, with the exception of actinidain from the juice of kiwi fruit, caused rapid loss of motility and digestion of the cuticle, leading to death of the nematode in vitro. In vivo, in contrast to the efficacy against H. polygyrus and T. muris, papaya latex only showed efficacy against P. muricola adult female worms when the stomach acidity had been neutralized prior to administration of papaya latex. Therefore, collectively, our studies have demonstrated that, with the appropriate formulation, plant cysteine proteinases have efficacy against nematodes residing throughout the rodent gastrointestinal tract.

Keywords

Protospirura muricolaplant cysteine proteinasespapayapineapplefigin vitroin vivoantacidanthelminticgastrointestinal nematodes
Type
Research Article
Information
Parasitology , Volume 134 , Issue 1 , January 2007 , pp. 103 - 112
Copyright
© 2006 Cambridge University Press

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