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An ion-sensitive microelectrode study on the effect of a high concentration of ivermectin on chloride balance in the somatic muscle bag cells of Ascaris suum

Published online by Cambridge University Press:  06 April 2009

H. R. Parri ,
M. B. A. Djamgoz ,
L. Holden-Dye  and
R. J. Walker
H. R. Parri
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO9 3TU, UK
M. B. A. Djamgoz
Affiliation:
Department of Applied Biology, Imperial College, London SW7 2BB, UK
L. Holden-Dye*
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO9 3TU, UK
R. J. Walker
Affiliation:
Department of Physiology and Pharmacology, University of Southampton, Bassett Crescent East, Southampton SO9 3TU, UK
*
*Reprint requests to Dr L. Holden-Dye.
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Summary

Ivermectin has been shown to increase chloride conductances of invertebrate cells. On the muscle cells of the parasitic nematode Ascaris, ivermectin acts as both a GABA receptor antagonist and a chloride channel opener. In this study, ion-sensitive microelectrodes were used to investigate the effect of ivermectin on intracellular C1− concentration of the somatic muscle bag cells of Ascaris suum. Incubation of muscle cells with ivermectin (10 μM in 1% dimethyl sulphoxide vehicle for 60 min) increased intracellular C1− by 2·9 mM or 15% compared to controls (P > 0·01, n = 6).

Keywords

ion-sensitive microelectrodeschlorideAscaris suumivermectin.
Type
Research Article
Information
Parasitology , Volume 106 , Issue 4 , May 1993 , pp. 421 - 427
Copyright
Copyright © Cambridge University Press 1993

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References

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