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An in vivo model for the study of chemotaxis induced by Schistosomula of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

L. P. Chao ,
J. T. Jones  and
J. R. Kusel
L. P. Chao
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
J. T. Jones
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
J. R. Kusel
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
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Summary

Peritoneal leucocytosis, with an increased percentage of eosinophils, was found in mice which had been infected with Schistosoma mansoni for 7 weeks or longer. Specific IgG against worm and egg antigens increased in peritoneal fluids and their corresponding sera respectively 5 and 7 weeks after infection. An intraperitoneal challenge with schistosomula elicited neutrophilia in all mice regardless of immune status, as well as infiltration of eosinophils and macrophages in infected mice. The secondary eosinophilia occurred in mice previously infected for 1 week or longer, whereas the infiltration of macrophages occurred only after worms from the primary infection had started laying eggs. Unlike the eosinophilia the macrophage response required infection with bisexual populations of cercariae. Injection of previously infected mice with Escherichia, Trichinella or Toxocara failed to increase the proportions of eosinophils and macrophages. Schistosomula-induced eosinophilia could be elicited in passively sensitized mice. Intraperitoneal injection of PBS extract of adult worms elicited eosinophilia in infected mice and neutrophilia in normal mice. Two chromatographic fractions induced eosinophilia and the third only neutrophilia. The relevance of these observations to host response to S. mansoni infections is discussed.

Type
Research Article
Information
Parasitology , Volume 92 , Issue 1 , February 1986 , pp. 117 - 132
Copyright
Copyright © Cambridge University Press 1986

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