Hostname: page-component-586b7cd67f-dsjbd Total loading time: 0 Render date: 2024-11-23T14:44:55.371Z Has data issue: false hasContentIssue false
  • English
  • Français

An in vivo model for the study of chemotaxis induced by Schistosomula of Schistosoma mansoni

Published online by Cambridge University Press:  06 April 2009

L. P. Chao ,
J. T. Jones  and
J. R. Kusel
L. P. Chao
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
J. T. Jones
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
J. R. Kusel
Affiliation:
Department of Biochemistry, University of Glasgow, Glasgow G12 8QQ
Get access Rights & Permissions [Opens in a new window]

Summary

Peritoneal leucocytosis, with an increased percentage of eosinophils, was found in mice which had been infected with Schistosoma mansoni for 7 weeks or longer. Specific IgG against worm and egg antigens increased in peritoneal fluids and their corresponding sera respectively 5 and 7 weeks after infection. An intraperitoneal challenge with schistosomula elicited neutrophilia in all mice regardless of immune status, as well as infiltration of eosinophils and macrophages in infected mice. The secondary eosinophilia occurred in mice previously infected for 1 week or longer, whereas the infiltration of macrophages occurred only after worms from the primary infection had started laying eggs. Unlike the eosinophilia the macrophage response required infection with bisexual populations of cercariae. Injection of previously infected mice with Escherichia, Trichinella or Toxocara failed to increase the proportions of eosinophils and macrophages. Schistosomula-induced eosinophilia could be elicited in passively sensitized mice. Intraperitoneal injection of PBS extract of adult worms elicited eosinophilia in infected mice and neutrophilia in normal mice. Two chromatographic fractions induced eosinophilia and the third only neutrophilia. The relevance of these observations to host response to S. mansoni infections is discussed.

Type
Research Article
Information
Parasitology , Volume 92 , Issue 1 , February 1986 , pp. 117 - 132
Copyright
Copyright © Cambridge University Press 1986

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

REFERENCES

Askenase, P. W., Hayden, B. J. & Higashi, G. I. (1976). Cutaneous basophil hypersensitivity and inhibited macophage migration in guinea pigs with schistosomiasis. Clinical and Experimental Immunology 23, 318–27.Google Scholar
Colley, D. G., Magalhaes-Filho, A. & Coelho, R. B. (1972). Immunopathology of dermal reactions induced by Schistosoma mansoni cercariae and cercarial extract. American Journal of Tropical Medicine and Hygiene 21, 558–77.CrossRefGoogle ScholarPubMed
Da Silva, W. D. & Kazatchkine, M. D. (1980). Schistosoma mansoni: activation of the alternative pathway of human complement by schistosomula. Experimental Parasitology 50, 278–86.CrossRefGoogle Scholar
Denham, D. A. (1965). Studies with methyridine and Trichinella spiralis. I. Effect upon the intestinal phase in mice. Experimental Parasitology 17, 10–11.CrossRefGoogle ScholarPubMed
De Savigny, D. H. (1975). In vitro maintenance of Toxocara canis larvae and a simple method for the production of Toxocara Es antigen for use in serodiagnostic tests for visceral larval migrans. Journal of Parasitology 61, 781–2.CrossRefGoogle Scholar
Fishel, C. W., Halkias, D. G., Klein, T. W. & Szentivanyi, A. (1976). Characteristics of cells present in peritoneal fluids of mice injected intraperitoneally with Bordetella pertussis. Infection and Immunity 13, 262–72.CrossRefGoogle ScholarPubMed
Hopper, K. E. & Nelson, D. S. (1979). Specific triggering of macrophage accumulation at the site of secondary tumor challenge in mice with concomitant tumor immunity. Cellular Immunology 47, 163–9.CrossRefGoogle ScholarPubMed
James, S. L., Correa-Oliveira, R. & Leonard, E. J. (1984). Defective vaccine-induced immunity to Schistosoma mansoni in P strain mice. II. Analysis of cellular responses. Journal of Immunology 133, 1598–3.Google Scholar
Kopaniak, M., Issekutz, A. C. & Movat, H. Z. (1980). Kinetics of acute inflammation induced by Escherichia coli in rabbit. American Journal of Pathology 98, 485–93.Google Scholar
Lichtenberg, F. von & Ritchie, L. S. (1961). Cellular resistance against schistosomula of Schistosoma mansoni in Macaca mulatta monkeys followed prolonged infections. American Journal of Tropical Medicine and Hygiene 10, 859–69.CrossRefGoogle Scholar
Lichtenberg, F. Von, Sher, A., Gibbons, N. & Doughty, B. L. (1976). Eosinophil-enriched inflammatory response to Schistosoma in the skin of mice immune to Schistosoma mansoni. American Journal of Pathology 84, 479–500.Google ScholarPubMed
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
Maarsseveen, A. C. M. Th. (1977). Presence and production of migration inhibitory activity in the peritoneal cavity. Immunology 32, 893–8.Google ScholarPubMed
McLaren, M., Draper, C. C., Roberts, J. M., Minter-Goedbloed, E., Lighthart, G. S., Teesdale, C. H., Amin, M. A., Omer, A. H. S., Bartlett, A. & Voller, A. (1978). Studies on the enzyme linked immunosorbent assay (ELISA) test for Schistosoma mansoni infections. Annals of Tropical Medicine and Parasitology 72, 243–53.CrossRefGoogle Scholar
McLaren, D. J., Pearce, E. J. & Smithers, S. R. (1985). Site potential for challenge attrition in mice, rats and guinea pigs vaccinated with irradiated cercariae of Schistosoma mansoni. Parasite Immunology 7, 29–44.CrossRefGoogle ScholarPubMed
Phillips, S. M. & Colley, D. G. (1978). Immunologic aspect of host response to schistosomiasis, immunopathology and eosinophil involvement. Progress in Allergy 24, 49–182.Google ScholarPubMed
Postelthwaite, A. E. & Snyderman, R. S. (1975). Characterization of chemotactic activity produced in vivo by a cell-mediated immune reaction in the guinea pig. Journal of Immunology 114, 274–8.CrossRefGoogle Scholar
Ramalho-Pinto, F. J., Gazzinelli, G., Howells, R. E., Mota-Santos, T. A. & Figueiredo, E. A. (1974). Schistosoma mansoni: defined system for stepwise transformation of cercaria to schistosomula in vitro. Experimental Parasitology 36, 360–72.CrossRefGoogle Scholar
Santoro, F., Lachmann, P. J., Capron, A. & Capron, M. (1979). Activation of complement by Schistosoma mansoni schistosomula: killing of parasites by the alternative pathway and requirement of IgG for classical pathway activation. Journal of Immunology 123, 1551–7.CrossRefGoogle ScholarPubMed
Smith, M. A. (1975). Ph.D. thesis, University of London and University of Brunel (Middlesex).Google Scholar
Smithers, S. R. & Terry, R. J. (1976). The immunology of schistosomiasis. In Advances in Parasitology, vol. 14 (ed. Dawes, B.), pp. 399–422. London: Academic Press.Google Scholar
Stevenson, P. & Jacobs, D. E. (1977). Toxocara infection in pigs. The use of indirect fluorescent antibody tests and an in vitro larval precipitate test for detecting specific antibodies. Journal of Helminthology 51, 149–54.CrossRefGoogle Scholar
Tavares, C. A. P., Gazzinelli, G., Mota-Santos, T. A. & Da Silva, D. W. (1978). Schistosoma mansoni: Complement-mediated cytotoxic activity in vitro and effect of decomplementation on acquired immunity in mice. Experimental Parasitology 46, 145–51.CrossRefGoogle ScholarPubMed
Taylor, M. G., Amin, M. B. A. & Nelson, G. S. (1969). ‘Parthenogenesis’ in Schistosoma mattheei. Journal of Helminthology 43, 197–206.CrossRefGoogle ScholarPubMed
Valone, G. H. & Goetzl, E. J. (1978). Immunologica release in the rat peritoneal cavity of lipid chemotactic and chemokinetic factors for polymorphonuclear leukocytes. Journal of Immunology 120, 102–8.CrossRefGoogle Scholar