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An immunocytochemical study with Fasciola hepatica

Published online by Cambridge University Press:  06 April 2009

E. Thorpe
E. Thorpe
Affiliation:
Imperial Chemical Industries Limited, Pharmaceuticals Division, Alderley Park, Cheshire
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Extract

The results are described of a study of the distribution of the antigens in F. hepatica at various stages of development, and in the liver and spleen of experimentally infected rats using globulins labelled with fluorescein isothiocyanate, from rats and sheep infected with F. hepatica.

Possible uses of this technique in aiding the diagnosis of fascioliasis are indicated.

I wish to thank Dr A. W. J. Broome for a supply of the metacercariae of F. hepatica, and Dr J. S. S. Lowe who kindly isolated and labelled the globulins with fluorescein isothiocyanate. Miss Enid Eccleston gave valuable technical assistance.

Type
Research Article
Information
Parasitology , Volume 55 , Issue 2 , May 1965 , pp. 209 - 214
Copyright
Copyright © Cambridge University Press 1965

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