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Alternative energy production pathways in Taenia crassiceps cysticerci in vitro exposed to a benzimidazole derivative (RCB20)

Published online by Cambridge University Press:  28 December 2015

CAROLINA MIGUEL FRAGA ,
TATIANE LUIZA DA COSTA ,
ANA MARIA DE CASTRO ,
OLIVIA REYNOSO-DUCOING ,
JAVIER AMBROSIO ,
ALICIA HERNÁNDEZ-CAMPOS ,
RAFAEL CASTILLO  and
MARINA CLARE VINAUD
CAROLINA MIGUEL FRAGA
Affiliation:
Laboratory of Studies of the Host-Parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias
TATIANE LUIZA DA COSTA
Affiliation:
Laboratory of Studies of the Host-Parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias
ANA MARIA DE CASTRO
Affiliation:
Laboratory of Studies of the Host-Parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias
OLIVIA REYNOSO-DUCOING
Affiliation:
Laboratorio de Biologia del Citoesqueleto, Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (UNAM), México, DF 04510, Mexico
JAVIER AMBROSIO
Affiliation:
Laboratorio de Biologia del Citoesqueleto, Departamento de Microbiologia y Parasitologia, Facultad de Medicina, Universidad Nacional Autonoma de Mexico (UNAM), México, DF 04510, Mexico
ALICIA HERNÁNDEZ-CAMPOS
Affiliation:
Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México (UNAM), México, DF 04510, Mexico
RAFAEL CASTILLO
Affiliation:
Facultad de Química, Departamento de Farmacia, Universidad Nacional Autónoma de México (UNAM), México, DF 04510, Mexico
MARINA CLARE VINAUD*
Affiliation:
Laboratory of Studies of the Host-Parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias
*
* Corresponding author: Laboratory of Studies of the Host-Parasite Relationship, Tropical Pathology and Public Health Institute, Federal University of Goias, Rua 235, s/n, Setor Universitário, Goiania, Goias, CEP:74605-050, Brazil. E-mail: [email protected]
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Summary

Biochemical studies of benzimidazole derivatives are important to determine their mode of action and activity against parasites. The lack of antihelminthic alternatives to treat parasitic infections and albendazole resistance cases make the search for new antiparasitary drugs of utmost importance. The 6-chloro-5-(1-naphthyloxy)-2-(trifluoromethyl)-1H-benzimidazole (RCB20) is a benzimidazole derivative with promising effect. This study evaluated the effect of different concentrations of RCB20 in the alternative energetic pathway of in vitro Taenia crassiceps cysticerci. The parasites were in vitro exposed to 6·5 and 13 µ m of RCB20 and albendazole sulfoxide (ABZSO). The quantification of acetate, acetoacetate, β-hydroxybutyrate, fumarate and propionate was performed by high-performance liquid chromatography. The quantification of urea, creatinine and total proteins was performed by spectrophotometry. The increase in β-hydroxybutyrate reflects the enhancement of the fatty acid oxidation in the treated groups. Volatile fatty acids secretion, acetate and propionate, was increased in the treated groups. The secretion mechanisms of the treated parasites were impaired due to organic acids increased concentrations in the cysticerci. It is possible to conclude that the metabolic effect on alternative energetic pathways is slightly increased in the parasites treated with RCB20 than the ones treated with ABZSO.

Keywords

Taenia crassicepsketonic bodiesfatty acids oxidationprotein catabolismbenzimidazole derivative
Type
Research Article
Information
Parasitology , Volume 143 , Issue 4 , April 2016 , pp. 488 - 493
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Copyright
Copyright © Cambridge University Press 2015 

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