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Aegyptianella pullorum Carpano 1928—fine structure and taxonomy

Published online by Cambridge University Press:  06 April 2009

R. G. Bird  and
P. C. C. Garnham
R. G. Bird
Affiliation:
London School of Hygiene and Tropical Medicine
P. C. C. Garnham
Affiliation:
London School of Hygiene and Tropical Medicine
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Extract

Intraerythrocytic granules in the blood of Sudanese fowls first seen by Balfour (1908) were named Aegyptianella pullorum by Carpano (1928).

Electron microscopy of a Rhodesian strain of A. pullorum indicated the similarity between these organisms and the Rickettsia-PLT group of organisms, also their dissimilarity to Sporozoa which we have studied. The organism was coccoid or coccobacillary, measuring up to 0·6 μm in diameter, and in longitudinal sections 1·5–3 μm. Pleomorphic and dividing forms were observed. Each organism was surrounded by two unit membranes with short internal invaginations from the inner membrane only. A layer of ribosome-like particles varying in density and depth gave the impression of an outer cortex. Irregular extensions of this layer towards the centre of the organism followed the lines of fine DNA-like filaments forming a web lattice throughout the substance of the organism. There was no well-defined nucleus as seen in Protozoa. Single as well as groups of organisms were situated in vacuoles lined by a membrane similar to the erythrocytes' own outer membrane. No intranuclear organisms were seen.

The similarity of these organisms to symbionts studied by Roshdy and Bird in both adult and immature stages of A. persicus is illustrated. From this evidence, and its apparent likeness to the tick-borne fever agent, there is good reason for including A. pullorum among the Rickettsiaceae. The position suggested for it is between the Rickettsia and PLT group of organisms, closely allied to Wolbachia, the agent of tick-borne fever (Cytoecetes sp.) and the agent of bovine heart water disease (Cowdria ruminantium).

We thank Mr R. Jacobson, F.I.M.L.T. for his willing assistance in conducting the sensitivity test, also Miss L. Lanigan and Mr D. Day for their technical and photographic assistance.

Type
Research Article
Information
Parasitology , Volume 59 , Issue 4 , November 1969 , pp. 745 - 752
Copyright
Copyright © Cambridge University Press 1969

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