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Adherence of Helicobacter pylori to Opisthorchis viverrini gut epithelium and the tegument mediated via L-fucose binding adhesin

Published online by Cambridge University Press:  29 April 2022

Prissadee Thanaphongdecha ,
Yaovalux Chamgramol ,
Chawalit Pairojkul ,
Raksawan Deenonpoe ,
Sutas Suttiprapa Open the ORCID record for Sutas Suttiprapa [Opens in a new window] ,
Paul J. Brindley  and
Banchob Sripa Open the ORCID record for Banchob Sripa [Opens in a new window]
Prissadee Thanaphongdecha
Affiliation:
Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Yaovalux Chamgramol
Affiliation:
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Chawalit Pairojkul
Affiliation:
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Raksawan Deenonpoe
Affiliation:
Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Sutas Suttiprapa
Affiliation:
Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
Paul J. Brindley
Affiliation:
Department of Microbiology, Immunology and Tropical Medicine, and Research Center for Neglected Tropical Diseases of Poverty, School of Medicine & Health Sciences, The George Washington University, Washington DC 20037, USA
Banchob Sripa*
Affiliation:
Tropical Disease Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand
*
Author for correspondence: Banchob Sripa, E-mail: [email protected]
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Abstract

Recent reports implicate both the liver fluke Opisthorchis viverrini as a reservoir of Helicobacter pylori within the human gastrointestinal tract and H. pylori in the pathogenesis of opisthorchiasis-associated cholangiocarcinoma. We postulated that adherence of bacterial ligands to host receptors initiates colonization of the live fluke by H. pylori and here we aimed to assess the molecular interaction between O. viverrini and H. pylori by investigating host receptors for H. pylori in the fluke. Several known receptors of H. pylori including Lewis B, sialyl-Lewis X, Toll-like receptor 4 and L-fucose were detected immunohistochemically and histochemically by focusing analysis on the gut epithelium and tegument of the adult stage of the fluke. The frequency of detection of Lewis B, sialyl-Lewis X, TLR4 and L-fucose in 100 individual worms was 3, 3, 19 and 70%, respectively. Detection of H. pylori by a diagnostic ureA gene-based PCR assay revealed the presence of H. pylori in individual O. viverrini worms in 41 of 49 (79%) worms examined. In addition, numbers of bacteria decreased in a dose- and time-dependent fashion following exposure to fucosidase. These findings suggested that L-fucose represents a tractable receptor for H. pylori that can mediate bacterial colonization of the gut of O. viverrini.

Keywords

Glycan receptorsHelicobacter pylorihost–pathogen interactionsL-fucoseOpisthorchis viverrini
Type
Research Article
Information
Parasitology , Volume 149 , Special Issue 10: Foodborne Trematodes – time to rise from neglected status , September 2022 , pp. 1374 - 1379
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Copyright
Copyright © The Author(s), 2022. Published by Cambridge University Press

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