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Active entry of bloodstream forms of Trypanosoma cruzi into macrophages

Published online by Cambridge University Press:  06 April 2009

T. L. Kipnis ,
V. L. G. Calich  and
W. Dias da Silva
T. L. Kipnis
Affiliation:
Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Univereidade de São Paulo, São Paulo, Brasil
V. L. G. Calich
Affiliation:
Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Univereidade de São Paulo, São Paulo, Brasil
W. Dias da Silva
Affiliation:
Departamento de Microbiologia e Imunologia, Instituto de Ciências Biomédicas, Univereidade de São Paulo, São Paulo, Brasil
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Summary

The uptake of bloodstream forms of Trypanosoma cruzi, Y and CL stocks, by mouse peritoneal macrophages and their intracellular differentiation and multiplication has been compared in vitro. After 48 h the number of macrophages showing intracellular amastigote forms was higher when the Y stock was used. The number of parasitized cells increased with the time of contact between parasites and macrophages. Prior treatment of the parasites with anti-T. cruzi antibodies and/or complement increased the number of infected macrophages, but did not interfere with their subsequent differentiation within the macrophages. The number of parasitized cells was greater when macrophages were obtained from mice previously treated with lipopolysaccharide, peptone or thioglycollate. Uptake was not appreciably affected when macrophages were pre-treated with trypsin or anti-macrophage serum, or when the parasites and macrophages were incubated in the presence of cytochalasin B. In the same experimental conditions, epimastigotes of T. cruzi were not able to differentiate into amastigotes. Their uptake was potentiated by previous treatment with specific antibodies and/or complement and was blocked by cytochalasin B. These results confirm that epimastigotes derived from T. cruzi cultures are phagocytosed and suggest that bloodstream forms penetrate actively into macrophages.

Type
Research Article
Information
Parasitology , Volume 78 , Issue 1 , February 1979 , pp. 89 - 98
Copyright
Copyright © Cambridge University Press 1979

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