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Activated macrophages in highly irradiated cercariae-induced immunity to Schistosoma japonicum in rats

Published online by Cambridge University Press:  06 April 2009

C. Xu
Affiliation:
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, No. 1 Lane 106, Shi-Long Road, Shanghai 200030, People's Republic of, China
S. Xu
Affiliation:
Shanghai Institute of Animal Parasitology, Chinese Academy of Agricultural Sciences, No. 1 Lane 106, Shi-Long Road, Shanghai 200030, People's Republic of, China

Summary

The results of studies on the schistosomulicidal activity of activated peritoneal and alveolar macrophages (pMø and aMø) from rats immunized with highly irradiated (50 krad.) Schistosoma japonicum cercariae are reported. The authors have examined the activation of these macrophages in terms of spreading, adhesion and ingestion of sheep erythrocytes and pinocytosis of horse-radish peroxidase. Using three criteria, peritoneal macrophages and alveolar macrophages from immunized rats and from rats intraperitoneally injected with BCG were significantly more active than those from normal rats or rats stimulated with 10% proteose-peptone or 1% sodium thioglycolate. A significantly higher percentage of adhesion and ingestion was obtained with the sheep erythrocytes that were co-opsonized by heat-inactivated rat anti-sheep erythrocyte serum and fresh normal rat serum. Schistosomulicidal effects were observed with macrophages from irradiated cercariae-immunized rats in two activation systems: in vitro activation in the presence of macrophage-activating factor (MAF), and in vivo activation by the intraperitoneal challenge with sonicated cercarial antigens.

Type
Research Article
Copyright
Copyright © Cambridge University Press 1991

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References

Alevy, Y. G., Hutcheson, P., Mueller, K. R. & Slavin, R. G. (1983). Suppressor alveolar macrophages in experimentally induced uremia. Journal of the Reticuloendothelial Society 33, 1120.Google ScholarPubMed
Bianco, C., Eden, A. & Cohn, Z. A. (1976). The induction of macrophage spreading: role of coagulation factors and the complement system. Journal of Experimental Medicine 144, 1531–44.CrossRefGoogle ScholarPubMed
Bianco, C., Griffin, F. M. & Silverstein, S. C. (1975). Studies of the macrophage complement receptor. Alteration of receptor function upon macrophage activation. Journal of Experimental Medicine 141, 1278–90.CrossRefGoogle ScholarPubMed
Borashi, D. & Meltzer, M. S. (1979). Defective tumoricidal capacity of macrophages from A/J mice. II. Comparison of the macrophage defect of A/J mice with that of lipid-A unresponsive C3H/HeJ mice. Journal of Immunology 122, 1592–7.Google Scholar
Bout, D. T., Dupas, H., Carlier, Y., Afchain, D. & Capron, A. (1977). High resistance induced by young live Bacillus Calmette-Guérin (BCG) to Schistosoma mansoni infection in mice. Annals of Immunology 128C, 811–16.Google Scholar
Bout, D. T., Joseph, M., David, J. R. & Capron, A. R. (1981). In vitro killing of S. mansoni schistosomula by lymphokine-activated mouse macrophages. Journal of Immunology 127, 15.CrossRefGoogle ScholarPubMed
Clegg, J. A. & Smithers, S. R. (1972). The effects of immune rhesus monkey serum on schistosomula of Schistosoma mansoni during cultivation in vitro. International Journal for Parasitology 2, 7998.CrossRefGoogle ScholarPubMed
Edelson, P. J., Zwiebel, R. & Cohn, Z. A. (1975). The pinocytic rate of activated macrophages. Journal of Experimental Medicine 142, 1150–64.CrossRefGoogle ScholarPubMed
Gordon, S., Todd, J. & Cohn, Z. A. (1974). In vitro synthesis and secretion of lysozyme by mononuclear phagocytes. Journal of Experimental Medicine 139, 1228–48.CrossRefGoogle ScholarPubMed
James, S. L. & Glaven, J. (1989). Macrophage cytotoxicity against schistosomula of Schistosoma mansoni involves arginine-dependent production of reactive nitrogen intermediates. Journal of Immunology 143, 4208–12.CrossRefGoogle ScholarPubMed
James, S. L., Lazdins, J. K., Hieny, S. & Natovitz, P. (1983 b). Macrophages as effector cells of protective immunity in murine schistosomiasis. VI. T cell-dependent, lymphokine-mediated, activation of macrophages in response to Schistosoma mansoni antigens. Journal of Immunology 131, 1481–6.CrossRefGoogle Scholar
James, S. L., Lazdins, J. K., Meltzer, M. S. & Sher, A. (1982 a). Macrophages as effector cells of protective immunity in murine schistosomiasis. I. Activation of peritoneal macrophages during natural infection. Cellular Immunology 67, 255–66.CrossRefGoogle Scholar
James, S. L., Leonard, E. J. & Meltzer, M. S. (1982 b). Macrophages as effector cells of protective immunity of murine schistosomiasis. IV. Coincident induction of macrophage activation for extracellular killing of schistosomula and tumor cells. Cellular Immunology 74, 8696.CrossRefGoogle ScholarPubMed
James, S. L., Natovitz, P. C., Farrar, W. L. & Leonard, E. J. (1984). Macrophages as effector cells of protective immunity in murine schistosomiasis: macrophage activation in mice vaccinated with radiation-attenuated cercariae. Infection and Immunity 44, 569–75.CrossRefGoogle ScholarPubMed
James, S. L. & Sher, A. (1983 a). Macrophages as effector cells of protective immunity in murine schistosomiasis. III. Identification of a mouse strain, P/N, that fails to respond to vaccination. Parasite Immunology 5, 567–75.CrossRefGoogle Scholar
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
McLaren, D. J. (1984). Disguise as an evasive stratagem of parasite organisms. Parasitology 88, 597611.CrossRefGoogle ScholarPubMed
Mahmoud, A. A. F., Peters, P. A. S., Civil, R. H. & Remington, J. S. (1979). In vitro killing of schistosomula of Schistosoma mansoni by BCG and Corynebacterium parvum-activated macrophages. Journal of Immunology 122, 1655–7.CrossRefGoogle Scholar
Mangold, B. L. & Dean, D. A. (1983). Autoradiographic analysis of Schistosoma mansoni migration from skin to lungs in naive mice. Evidence that most attrition occurs after the skin phase. American Journal of Tropical Medicine and Hygiene 32, 785–9.CrossRefGoogle ScholarPubMed
Nacy, C. A. & Meltzer, M. S. (1979). Macrophage in resistance to rickettsial infection: macrophage activation in vitro for killing of Rickettsia tsutsugamushi. Journal of Immunology 123, 2544–9.CrossRefGoogle ScholarPubMed
Nacy, C. A., Fortier, A. H., Meltzer, M. S., Buchmeier, N. A. & Schreiber, R. D. (1985). Macrophage activation to kill Leishmania major: activation of macrophages for intracellular destruction of amastigotes can be induced by both recombinant gamma interferon and non-interferon lymphokine. Journal of Immunology 135, 3505–11.CrossRefGoogle Scholar
Nibbering, P. H., Zomerdijk, T. P. L., CorsÈl-Van Tilburg, A. J. & Furth, R. V. (1990), Mean cell volume of human blood leucocytes and resident and activated murine macrophages. Journal of Immunological Methods 129, 143–5.CrossRefGoogle ScholarPubMed
Pearce, E. J. & James, S. L. (1986). Post-lung stage schistosomula of Schistosoma mansoni exhibit transient susceptibility to macrophage-mediated cytotoxicity in vitro that may relate to late phase killing in vivo. Parasite Immunology 8, 513–27.CrossRefGoogle ScholarPubMed
Ruco, L. P. & Meltzer, M. S. (1977). Macrophage activation for tumor cytotoxicity: induction of tumoricidal macrophages by PPD-stimulated Bacillus Calmette Guérin immune spleen cell cultures. Journal of Immunology 119, 889–96.CrossRefGoogle ScholarPubMed
Someya, A. (1986). A novel rat monoclonal antibody reactive with murine tumoricidal Kupffer cells and activated peritoneal macrophages from BCG-infected mice. Immunology 57, 605–10.Google ScholarPubMed
Unkeless, J. C., Gordon, S. & Reich, E. (1974). Secretion of plasminogen activator by stimulated macrophages. Journal of Experimental Medicine 139, 834–50.CrossRefGoogle ScholarPubMed
Werb, Z. & Gordon, S. (1975 a). Secretion of a specific collagenase by stimulated macrophages. Journal of Experimental Medicine 142, 346–60.CrossRefGoogle ScholarPubMed
Werb, Z. & Gordon, S. (1975 b). Elastinase secretion by stimulated macrophages. Characterization and regulation. Journal of Experimental Medicine 142, 361–77.CrossRefGoogle Scholar
Xu, C. B. (1987). Experimental studies on the highly irradiated Schistosoma japonicum cercariae-induced immunity in rats (in Chinese, with English abstract). Ph. D. thesis, Shanghai, People's Republic of China.Google Scholar