Hostname: page-component-cd9895bd7-7cvxr Total loading time: 0 Render date: 2024-12-22T23:12:54.167Z Has data issue: false hasContentIssue false
  • English
  • Français

Use of accelerator mass spectrometry for studies in nutrition

Published online by Cambridge University Press:  14 December 2007

George S. Jackson ,
Connie Weaver  and
David Elmore
George S. Jackson
Affiliation:
Department of Physics, Purdue University, West Lafayette, IN 47907, USA
Connie Weaver
Affiliation:
Department of Foods and Nutrition, Purdue University, West Lafayette, IN 47907, USA
David Elmore*
Affiliation:
Department of Physics, Purdue University, West Lafayette, IN 47907, USA
*
*Corresponding author: Professor David Elmore, fax +1 765 496 7228, email [email protected]
Rights & Permissions [Opens in a new window]

Abstract

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

Accelerator mass spectrometry (AMS) is an ultrasensitive analytical technique for measuring rare nuclides such as 14C, 26Al and 41Ca. The low detection limit and wide dynamic range of AMS allow long-term and highly sensitive tracer studies in nutrition that cannot be performed with other methods. The present paper is intended to provide a description of AMS to the interested nutritionist and present proven applications. AMS is compared to liquid scintillation counting and stable isotope MS. A description of common AMS methodology is presented that consists of determining the dose, preparing the sample, diluting the sample (if necessary), and measuring the sample. Applications include Ca metabolism, Al uptake from the environment, dietary intake of carcinogens, fat meta-bolism and folate metabolism. Throughout this discussion the experimental advantages (small doses that pose no health risk, extremely long experimental lifetime, small sample sizes and high sensitivity) made possible by the unique analytical capabilities of AMS are emphasized. The future of AMS is discussed. As the number of AMS centres, instruments, and studies increases, the number of nutritional applications that employ AMS will continue to grow. The coupling of AMS with other analytical techniques (e.g. high performance liquid chromatography) will be developed as access to AMS improves.

Keywords

Accelerator mass spectrometryStable isotopesMicronutrient metabolismFat metabolismCarcinogenRadioisotope
Type
Research Article
Information
Nutrition Research Reviews , Volume 14 , Issue 2 , December 2001 , pp. 317 - 334
Copyright
Copyright © CABI Publishing 2001

References

Barker, J & Garner, RC (1999) Biomedical applications of accelerator mass spectrometry-isotope measurements at the level of the atom. Rapid Communications in Mass Spectrometry 13, 285–293.3.0.CO;2-R>CrossRefGoogle ScholarPubMed
Buchholz, BA, Arjomand, A, Dueker, SR, Schneider, PD, Clifford, AJ & Vogel, JS (1999) Intrinsic erythrocyte labeling and attomole pharmacokinetic tracing of 14C-labeled folic acid with accelerator mass spectrometry. Analytical Biochemistry 269, 348–352.CrossRefGoogle ScholarPubMed
Buchholz, BA, Fultz, E, Haack, KW, Vogel, JS, Gilman, DS, Gee, SJ, Hammock, BD, Hui, X, Wester, RC & Maibach, HI (1999) HPLC-accelerator MS measurement of atrazine metabolites in human urine after dermal exposure. Analytical Chemistry 71, 3519–3525.CrossRefGoogle ScholarPubMed
Carr, TEF, Harrison, GE and Nolan, J (1973) The long-term excretion and retention of an intravenous dose of 45Ca in two healthy men. Calcified Tissue Research 12, 217–226.CrossRefGoogle ScholarPubMed
Davis, JC (1994) AMS beyond 2000. Nuclear Instruments and Methods in Physics Research 92, 1–6.CrossRefGoogle Scholar
Day, JP, Barker, J, Evans, LJA, Perks, J, Seabright, PJ, Ackrill, P, Lilley, JS, Drumm, PV & Newton, GWA (1991) Aluminum absorption studied by 26Al tracer. Lancet 337, 1345.CrossRefGoogle ScholarPubMed
Day, JP, Barker, J, King, SJ, Miller, RV, Templar, J, Lilley, JS, Drumm, PV, Newton, GWA, Fifield, LK, Stone, JOH, Allan, GL, Edwardson, JA, Moore, PB, Ferrier, IN, Priest, ND, Newton, D, Talbot, RJ, Brock, JH, Sánchez, L, Dobson, CB, Itzhaki, RF, Radunovic, A & Bradbury, MWB (1994) Biological chemistry of aluminum using 26Al and accelerator mass spectrometry. Nuclear Instruments and Methods in Physics Research. 92, 463–468.CrossRefGoogle Scholar
Dingley, KH, Freeman, SPHT, Nelson, DO, Garner, CR & Turteltaub, KW (1998) Covalent binding of 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline to albumin and hemoglobin at environmentally relevant doses. Drug Metabolism and Disposition 26, 825–828.Google ScholarPubMed
Dingley, KH, Roberts, ML, Velsko, CA & Turteltaub, KW (1998) Attomole detection of 3H in biological samples using accelerator mass spectrometry: application in low-dose dual-isotope tracer studies in conjunction with 14C accelerator mass spectrometry. Chemical Research in Toxicology 11, 1217–1222.CrossRefGoogle ScholarPubMed
Dobson, CB, Day, PJ, King, SJ & Itzhaki, RF (1998) Location of aluminum and gallium in human neuroblastoma cells treated with metal-chelating agent complexes. Toxicology and Applied Pharmacology 152, 145–152.CrossRefGoogle ScholarPubMed
Dueker, SR, Lin, Y, Buchholz, BA, Schneider, PD, Lame, MW, Segall, HJ, Vogel, JS & Clifford, AJ (2000) Long-term kinetic study of β-carotene in a human subject using accelerator mass spectrometry in an adult volunteer. Journal of Lipid Research 41, 1790–1800.CrossRefGoogle Scholar
Edwardson, JA, Moore, PB, Ferrier, IN, Lilley, JS, Newton, GWA, Barker, J, Templar, J & Day, JP (1993) Effect of silicon on gastrointestinal absorption of aluminum. Lancet 342, 211–212.CrossRefGoogle Scholar
Elmore, D, Bhattacharyya, MH, Sacco-Gibson, N & Peterson, DP (1990) Calcium-41 as a long-term biological tracer for bone resorption. Nuclear Instruments and Methods in Physics Research 52, 531–535.CrossRefGoogle Scholar
Elmore, D & Phillips, FM (1987) Accelerator mass-spectrometry for measurement of long-lived radioisotopes. Science 236, 543–550.CrossRefGoogle ScholarPubMed
Fifield, LK (1999) Accelerator mass spectrometry and its applications. Reports on Progress in Physics 62, 1223–1274.CrossRefGoogle Scholar
Fink, D, Middleton, R, Klein, J & Sharma, P (1990) 41Ca: Measurement by accelerator mass spectrometry and applications. Nuclear Instruments and Methods in Physics Research 47, 79–96.CrossRefGoogle Scholar
Fink, D, Walton, J, Hotchkis, MAC, Jacobsen, GE, Lawson, EM, Smith, AM, Tuniz, C & Wilcox, D (1994) First 26Al analyses at the Antares AMS Centre: uptake via oral ingestion of 26Al in rats. Nuclear Instruments and Methods in Physics Research 92, 473–477.CrossRefGoogle Scholar
Flarend, R & Elmore, D (1997) Aluminum-26 as a tracer using accelerator mass spectrometry. In Aluminum Toxicity in Infants Health and Disease, pp. 16–39 [Zatta, P & Alfrey, A, editors]. River Edge, NJ: World Scientific Publishing Co.Google Scholar
Freeman, SPHT, Beck, B, Bierman, J, Caffee, MW, Heaney, RP, Holloway, L, Marcus, R, Southon, JR & Vogel, JS (2000) The study of skeletal Ca metabolism with 41Ca and 45Ca. Nuclear Instruments and Methods in Physics Research 172, 930–933.CrossRefGoogle Scholar
Freeman, SPHT, Serfass, RE, King, JC, Southon, JR, Fang, Y, Woodhouse, LR, Bench, GS & McAninch, JE (1995) Biological sample preparation and 41Ca AMS measurement at LLNL. Nuclear Instruments and Methods in Physics Research 99, 557–561.CrossRefGoogle Scholar
Freeman, SPHT & Vogel, JS (1995) Biomedical accelerator mass spectrometry. International Journal of Mass Spectrometry and Ion Processes 143, 247–256.CrossRefGoogle Scholar
Gilman, DS, Gee, SJ, Hammock, BD, Vogel, JS, Haack, K, Buchholz, BA, Freeman, SPHT, Wester, RC, Hui, X & Maibach, HI (1998) Analytical performance of accelerator mass spectrometry and liquid scintillation counting for detection of 14C-labelled atrazine metabolites in human urine. Analytical Chemistry 70, 3463–3469.CrossRefGoogle Scholar
Heaney, RP (2000) Factors influencing the measurement of bioavailibility, taking calcium as a model. Journal of Nutrition 131, 13445–13485.Google Scholar
Hohl, C, Gerisch, P, Korschinek, G & Nolte, E (1994) Medical application of 26Al. Nuclear Instruments and Methods in Physics Research 92, 478–482.CrossRefGoogle Scholar
Hughey, BJ, Klinkowstein, RE, Shefer, RE, Skipper, PL, Tannenbaum, SR & Wishnok, JS (1997) Design of a compact 1 MV AMS system for biomedical research. Nuclear Instruments and Methods in Physics Research 123, 153–158.CrossRefGoogle Scholar
Jackson, GS, Elmore, D, Rickey, FA, Musameh, SA, Sharma, P, Hillegonds, D, Coury, L & Kissinger, P (2000) The PRIME Lab Biomedical Program. Nuclear Instruments and Methods in Physics Research 172, 901–905.Google Scholar
Jackson, GS, Rickey, FA, Hillegonds, D, Rounds, MA & Elmore, D (2000) Attomole measurement of labelled compounds. Current Separations 19, 3–8.Google Scholar
Jacob, SAW, Suter, M & Synal, H-A (2000) Ion beam interaction with stripper gas-Key for AMS at sub MeV. Nuclear Instruments and Methods in Physics Research 172, 235–241.CrossRefGoogle Scholar
Johnson, RR, Berkovits, D, Boaretto, E, Gelbart, Z, Ghelberg, S, Meirav, O, Paul, M, Prior, J, Sossi, V & Venczel, E (1994) Calcium resorption from bone in a human studied by 41Ca tracing. Nuclear Instruments and Methods in Physics Research 92, 483–488.CrossRefGoogle Scholar
Johnson, RR, Berkovits, D, Boaretto, E, Gelbart, Z, Hershkowitz, A, Hui, SK, Paul, M, Prior, J & Venczel, E (1999 ) Upgrading of 41Ca AMS detection and biomedical application. 8th International Conference on Accelerator Mass SpectrometryVienna, Austria.Google Scholar
Jouhanneau, P, Lacour, B, Raisbeck, G, Yiou, F, Banide, H, Brown, E & Drüeke, T (1993) Gastrointestinal absorption of aluminum in rats using 26Al and accelerator mass spectrometry. Clinical Nephrology 40, 244–248.Google ScholarPubMed
Kieser, WE, Litherland, AE, Zhao, X-L, Kilius, LR & Beukens, RP (1996) A high capacity ion source for AMS. Nuclear Instruments and Methods in Physics Research 113, 461–464.CrossRefGoogle Scholar
King, SJ, Day, JP, Oldham, C, Popplewell, JF, Ackrill, P, Moore, PB, Taylor, GA, Edwardson, JA, Fifield, LK, Liu, K & Cresswell, RG (1997) The influence of dissolved silicate on the physiological chemistry of aluminum, studied in humans using tracer 26Al and accelerator mass spectrometry. Nuclear Instruments and Methods in Physics Research 123, 254–258.CrossRefGoogle Scholar
King, SJ, Oldham, C, Popplewell, JF, Carling, RS, Day, JP, Fifield, LK, Cresswell, RG, Liu, KX & diTada, ML (1997) Determination of aluminum-26 in biological materials by accelerator mass spectrometry. Analyst 122, 1049–1055.CrossRefGoogle Scholar
Kislinger, G, Steinhausen, C, Alvarez-Brückmann, M, Winklhofer, C, Ittel, T-H & Nolte, E (1997) Investigations of the human aluminum biokinetics with 26Al and AMS. Nuclear Instruments and Methods in Physics Research 123, 259–265.CrossRefGoogle Scholar
Kobayashi, K, Yumoto, S, Nagai, H, Hosoyama, Y, Imamura, M, Masuzawa, S, Koizumi, Y & Yamashita, H (1990) 26Al tracer experiment by accelerator mass spectrometry and its application to the studies for amyotrophic lateral sclerosis and alzheimer's disease. Proceedings of the Japan Academy 66, 189–192.CrossRefGoogle Scholar
Koslowsky, VT, Bray, N, Imahori, Y, Andrews, HR & Davies, WG (1997) The new Chalk River AMS ion source, sample changer and external sample magazine. Nuclear Instruments and Methods in Physics Research 123, 203–207.CrossRefGoogle Scholar
Liu, YF, Guo, ZY, Liu, XQ, Qu, T & Xie, JL (1994) Applications of accelerator mass-spectrometry in analysis of trace isotopes and elements. Pure and Applied chemistry 66, 305–334.CrossRefGoogle Scholar
Mauthe, RJ, Dingley, KH, Leveson, SH, Freeman, SPHT, Turesky, RJ, Garner, CR & Turteltaub, KW (1999) Comparison of DNA-adduct and tissue-available dose levels of MeIQx in human and rodent colon following administration of a very low dose. International Journal of Cancer 80, 539–545.3.0.CO;2-C>CrossRefGoogle ScholarPubMed
Mauthe, RJ, Snyderwine, EG, Ghoshal, A, Freeman, SPHT & Turteltaub, KW (1998) Distribution and metabolism of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in female rats and their pups at dietary doses. Carcinogenesis 19, 919–924.CrossRefGoogle ScholarPubMed
Mous, DJW, Purser, KH, Fokker, W, van den Broek, R & Koopmans, RB (1997) A compact 14C isotope ratio mass spectrometer for biomedical applications. Nuclear Instruments and Methods in Physics Research 123, 159–162.CrossRefGoogle Scholar
Patterson, KY & Veillon, C (2001). Stable isotopes of minerals as metabolic tracers in human nutrition research. Experimental Biology and Medicine 226 271–282.CrossRefGoogle ScholarPubMed
Phillips, DH, Farmer, PB, Beland, FA, Nath, RG, Poirer, MC, Reddy, MV & Turteltaub, KW (2000) Methods of DNA adduct determination and their application to testing compounds for genotoxicity. Environmental and Molecular Mutagenesis 35, 222–233.3.0.CO;2-E>CrossRefGoogle ScholarPubMed
Priest, ND, Newton, D, Day, JP, Talbot, RJ & Warner, AJ (1995) Human metabolism of aluminum-26 and gallium-67 as injected citrates. Human and Experimental Toxicology 14, 287–293.CrossRefGoogle Scholar
Priest, ND, Talbot, RJ, Newton, D, Day, JP, King, SJ & Fifield, LK (1998) Uptake by man of aluminum in a public water supply. Human and Experimental Toxicology 17, 296–301.CrossRefGoogle Scholar
Radunovic, A, Ueda, F, Raja, K, Day, JP, Templar, J & Bradbury, MWJ (1994) Uptake of 26Al and 67Ga into tissues of anaesthetized normal and hypotransferrinaemic mice. Journal of Physiology 475P, 154.Google Scholar
Reamer, DC & Veillon, C (1983) A double isotope dilution method for using stable selenium isotopes in metabolic tracer studies: Analysis by gas chromatography/mass spectrometry (GC/MS). Journal of Nutrition 113, 786.CrossRefGoogle ScholarPubMed
Rennie, MJ (1999) An introduction to the use of tracers in nutrition and metabolism. Proceedings of the Nutrition Society 58, 935–944.CrossRefGoogle Scholar
Rickey, FA, Elmore, D, Hillegonds, D, Badylak, S, Record, R & Simmons-Byrd, A (2000) Regeneration of tissue about an animal-based scaffold; AMS studies of the fate of the scaffold. Nuclear Instruments and Methods in Physics Research 172, 904–909.CrossRefGoogle Scholar
Ritschel, W.A. (1992) Handbook of Basic Pharmacokinetics - Including Clinical Applications. Hamilton, IL: Drug Intelligence Publications.Google Scholar
Stenström, K, Leide-Svegborn, S, Erlandsson, B, Hellborg, R, Sören, M, Nillson, L-E, Nosslin, B, Skog, G & Wiebert, A (1996) Application of accelerator mass spectrometry (AMS) for high-sensitivity measurements of 14CO2 in long-term studies of fat metabolism. Applied Radiation and Isotopes 47, 417–422.CrossRefGoogle ScholarPubMed
Suter, M, Jacob, S & Synal, HA (1997) AMS of 14C at low energies. Nuclear Instruments and Methods in Physics Research 123, 148–152.CrossRefGoogle Scholar
Synal, H-A, Jacob, S & Suter, M (2000) The PSI/ETH small radiocarbon dating system. Nuclear Instruments and Methods in Physics Research 172, 1–7.CrossRefGoogle Scholar
Talbot, RJ, Newton, D, Priest, ND, Austin, JG & Day, JP (1995) Inter-subject variability in the metabolism of aluminum following intravenous injection as citrate. Human and Experimental Toxicology 14, 595–599.CrossRefGoogle Scholar
Turteltaub, KW, Felton, JS, Gledhill, BL, Vogel, JS, Southon, JR, Cafee, MW, Finkel, RC, Nelson, DE, Proctor, ID & Davis, JC (1990) Accelerator mass spectrometry in biomedical dosimetry: relationship between low-level exposure and covalent binding of heterocyclic amine carcinogens to DNA. Proceedings of the National Academy of Sciences of the USA 87, 5288–5292.CrossRefGoogle ScholarPubMed
Turteltaub, KW, Vogel, JS, Frantz, C, Buonarati, MH & Felton, JS (1993) Low-level biological dosimetry of heterocyclic amine carcinogens isolated from cooked food. Environmental Health Perspectives 99, 183–186.CrossRefGoogle ScholarPubMed
Vogel, JS & Turteltaub, KW (1992) Biomolecular tracing through accelerator mass spectrometry. Trends in Analytical Chemistry 11, 142–149.CrossRefGoogle Scholar
Vogel, JS & Turteltaub, KW (1994) Accelerator mass spectrometry in biomedical research. Nuclear Instruments and Methods in Physics Research 92, 445–453.CrossRefGoogle Scholar
Vogel, JS & Turteltaub, KW (1998) Accelerator mass spectrometry as a bioanalytical tool for nutritional research. In Mathematical Modeling in Experimental Nutrition, pp. 397–410 [Müller, CA editor]. New York: Plenum Press.CrossRefGoogle Scholar
Vogel, JS, Turteltaub, KW, Felton, JS, Gledhill, BL, Nelson, DE, Southon, JR, Proctor, ID & Davis, JC (1990) Application of AMS to the biomedical sciences. Nuclear Instruments and Methods in Physics Research 52, 524–530.CrossRefGoogle Scholar
Vogel, JS, Turteltaub, KW, Finkel, R & Erle Nelson, D (1995) Accelerator mass spectrometry: isotope quantification at attomole sensitivity. Analytical Chemistry 67, A353–A359.CrossRefGoogle Scholar
Walker, VR, Sutton, RAL, Meirav, O, Sossi, V, Johnson, R, Klein, J, Fink, D & Middleton, R (1994) Tissue disposition of 26aluminum in rats measured by accelerator mass spectrometry. Clinical and Investigative Medicine 17, 420–425.Google ScholarPubMed
Weaver, C (1988) Istopic tracer methodology: potential in mineral nutrition. In Trace Minerals in Foods, pp. 429–454 [Smith, KT editor]. New York: Marcel Dekker.Google Scholar
Yokel, RA (2000) The toxicology of aluminum in the brain: a review. Neurotoxicology 21, 813–828.Google ScholarPubMed
Yokel, RA, Rhineheimer, SS, Brauer, RD, Sharma, P, Elmore, D & McNamara, PJ (2001) Aluminum bioavailability from drinking water is very low and is not appreciably influenced by stomach contents or water hardness. Toxicology 161, 93–101.CrossRefGoogle ScholarPubMed
Yumoto, S, Kakimi, S, Ogawa, Y, Nagai, H, Imamura, M and Kobayashi, K (1995) Aluminum neurotoxicity & Alzheimer's disease. In Alzheimer's and Parkinson's Diseases, pp. 223–229 [Hainin, I, Yoshida, M and Fisher, A editors]. New York: Plenum Press.CrossRefGoogle Scholar
Yumoto, S, Nagai, H, Imamura, M, Matsuzaki, H, Hayashi, K, Masuda, A, Kumazawa, H, Ohashi, H & Kobayashi, K (1997) 26Al uptake and accumulation in the rat brain. Nuclear Instruments and Methods in Physics Research 123, 279–282.CrossRefGoogle Scholar
Zafar, TA, Weaver, CM, Martin, BR, Flarend, R & Elmore, D (1997) Aluminum (26Al) metabolism in rats. Proceedings of the Society for Experimental Biology and Medicine 216, 81–85.CrossRefGoogle Scholar