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Potential nutritional and physiological functions of betaine in livestock

Published online by Cambridge University Press:  14 December 2007

M. Eklund ,
E. Bauer ,
J. Wamatu  and
R. Mosenthin
M. Eklund
Affiliation:
Institute of Animal Nutrition, University of Hohenheim, Emil-Wolff-Strasse 10, D-70599 Stuttgart, Germany
E. Bauer
Affiliation:
Institute of Animal Nutrition, University of Hohenheim, Emil-Wolff-Strasse 10, D-70599 Stuttgart, Germany
J. Wamatu
Affiliation:
Institute of Animal Nutrition, University of Hohenheim, Emil-Wolff-Strasse 10, D-70599 Stuttgart, Germany
R. Mosenthin*
Affiliation:
Institute of Animal Nutrition, University of Hohenheim, Emil-Wolff-Strasse 10, D-70599 Stuttgart, Germany
*
*Corresponding author: Dr Rainer Mosenthin, fax +49 711 459 2421, email [email protected]
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Abstract

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The present review summarises the potential nutritional and physiological functions of betaine as a feed additive in relation to performance criteria in livestock production. Betaine, the trimethyl derivative of the amino acid glycine, is a metabolite of plant and animal tissues. In plants, betaine is particularly synthesised and accumulated as an osmoprotectant against salt and temperature stress. In animals, betaine is the product of choline oxidation or it originates from nutritional sources. Over the past decades, numerous studies have been carried out to investigate the potential effects of betaine supplementation on animal performance. Due to its chemical structure, betaine shows the characteristics of a dipolar zwitterion resulting in osmoprotective properties. Promoting effects on the intestinal tract against osmotic stress occurring during diarrhoea or coccidiosis have been reported following betaine supplementation in pigs and poultry. There is also some evidence that dietary betaine may improve the digestibility of specific nutrients. As a product of choline oxidation, betaine is involved in transmethylation reactions of the organism. Betaine as a methyl donor provides its labile methyl groups for the synthesis of several metabolically active substances such as creatine and carnitine. Supplementation with betaine may decrease the requirement for other methyl donors such as methionine and choline. There is also some evidence for enhanced methionine availability after dietary supplementation of betaine resulting in improved animal performance. Alterations in the distribution pattern of protein and fat in the body have been reported following betaine supplementation. A more efficient use of dietary protein may result from a methionine-sparing effect of betaine, but also direct interactions of betaine with metabolism-regulating factors have to be considered. Though the mode of action of betaine as a carcass modifier remains open, there is, however, growing evidence that betaine could have a positive impact both on animal performance and carcass quality.

Keywords

BetaineOsmolytesMethyl donorsPerformanceCarcass quality
Type
Research Articles
Information
Nutrition Research Reviews , Volume 18 , Issue 1 , June 2005 , pp. 31 - 48
Copyright
Copyright © The Authors 2005

References

Alaviuhkola, T & Suomi, K (1990) Effect of betaine supplementation of low methionine diet for growing pigs. Annales Agriculturae Fenniae 29, 127–129.Google Scholar
Alfieri, RR, Cavazzoni, A, Petronini, PG, Bonelli, MA, Caccamo, AE, Borghetti, AF & Wheeler, KP (2002) Compatible osmolytes modulate the responses of porcine endothelial cells to hypertonicity and protect them from apoptosis. Journal of Physiology 540, 499–508.CrossRefGoogle ScholarPubMed
Augustine, PC & Danforth, HD (1999) Influence of betaine and salinomycin on intestinal absorption of methionine and glucose and on the ultrastructure of intestinal cells and parasite developmental stages in chicks infected with Eimeria acervulina. Avian Diseases 43, 89–97.CrossRefGoogle ScholarPubMed
Augustine, PC, McNaughton, JL, Virtanen, E & Rosi, L (1997) Effects of betaine on the growth performance of chicks inoculated with mixed cultures of avian Eimeria species and on invasion and development of Eimeria tenella and Eimeria acervulina in vitro and in vivo. Poultry Science 76, 802–809.CrossRefGoogle ScholarPubMed
Bagnasco, S, Balaban, R, Fales, HM, Yang, YM & Burg, M (1986) Predominant osmotically active organic solutes in rat and rabbit renal medullas. Journal of Biological Chemistry 261, 5872–5877.CrossRefGoogle ScholarPubMed
Bessieres, MA, Gibon, Y, Lefeuvre, JC & Larher, F (1999) A single step purification for glycine betaine determination in plant extracts by isocratic HPLC. Journal of Agricultural and Food Chemistry 47, 3718–3722.CrossRefGoogle ScholarPubMed
Blaxter, K (1989) The minimal metabolism. In Energy Metabolism in Animals and Man, pp. 120–145. Cambridge, MA: Cambridge University Press.Google Scholar
Campbell, RG, Morley, WC & Zabaras-Krick, B (1997) The effects of betaine on protein and energy metabolism in pigs. In Manipulating Pig In Production VI, p. 243 [Cranwell, PD, editor]. Werribee, Australia: Australian Pig Science Association.Google Scholar
Cera, KR & Schinckel, AP (1995) Carcass and performance responses to feeding betaine in pigs. Journal of Animal Science 73, Suppl. 1, 82.Google Scholar
Chambers, ST & Kunin, CM (1985) The osmoprotective properties of urine for bacteria: The protective effect of betaine and human urine against low pH and high concentrations of electrolytes, sugars, and urea. Journal of Infectious Diseases 152, 1308–1316.CrossRefGoogle Scholar
Chendrimada, TP, Garcia, NM, Pesti, GM, Davis, AJ & Bakalli, RI (2002) Determination of the betaine content of feed ingredients using high–performance liquid chromatography. Journal of the Science of Food and Agriculture 82, 1556–1563.CrossRefGoogle Scholar
Chung, CS, Etherton, TD & Wiggins, JP (1985) Stimulation of swine growth by porcine growth hormone. Journal of Animal Science 60, 118–130.CrossRefGoogle ScholarPubMed
Clarke, WC, Virtanen, E, Blackburn, J & Higgs, D (1994) Effects of a dietary betaine/amino acid additive on growth and seawater adaptation in yearling chinook salmon. Aquaculture 121, 137–145.CrossRefGoogle Scholar
Coma, J, Carrion, D & Zimmerman, DR (1995) Use of plasma urea nitrogen as a rapid response criterion to determine the lysine requirement of pigs. Journal of Animal Science 73, Suppl. 1, 82.CrossRefGoogle ScholarPubMed
Cromwell, GL, Lindemann, MD, Randolph, JR, Monegue, HJ, Laurent, KM & Parker, JR (1999) Efficacy of betaine as a carcass modifier in finishing pigs fed normal and reduced energy diets. Journal of Animal Science 77, Suppl. 1, 179.Google Scholar
Dawson, KM & Baltz, JM (1997) Organic osmolytes and embryos: substrates of the Gly and beta transport systems protect mouse zygote against the effects of raised osmolarity. Biology of Reproduction 56, 1550–1558.CrossRefGoogle ScholarPubMed
Dawson, RM, Grime, DW & Lindsay, DB (1981) On the insensitivity of sheep to the almost complete microbial destruction of dietary choline before alimentary-tract absorption. Biochemical Journal 196, 499–504.CrossRefGoogle Scholar
Emmert, JL, Garrow, TA & Baker, DH (1996) Hepatic betaine–homocysteine methyltransferase activity in the chicken is influenced by dietary intake of sulfur amino acids, choline and betaine. Journal of Nutrition 126, 2050–2058.Google ScholarPubMed
Emmert, JL, Webel, DM, Biehl, RR, Griffiths, MA, Garrow, LS, Garrow, TA & Baker, DH (1998) Hepatic and renal betaine-homocysteine methyltransferase activity in pigs as affected by dietary intakes of sulfur amino acids, choline, and betaine. Journal of Animal Science 76, 606–610.CrossRefGoogle ScholarPubMed
Esteve-Garcia, E & Mack, S (2000) The effect of DL-methionine and betaine on growth performance and carcass characteristics in broilers. Animal Feed Science and Technology 87, 85–93.CrossRefGoogle Scholar
Etherton, TD, Wiggins, JP, Chung, CS, Evock, CM, Rebhun, JF & Walton, PE (1986) Stimulation of pig growth performance by porcine growth hormone and growth hormone-releasing factor. Journal of Animal Science 63, 1389–1399.CrossRefGoogle ScholarPubMed
EU–Safety Data, Sheet (1999 a) Product Name: Betaine Hydrochloride. http://www.aminoactives.com/pdf/eumsds/BetaineHydrochloride.pdfGoogle Scholar
EU–Safety Data Sheet (1999 b) Product Name: Betaine. http://www.aminoactives.com/pdf/eumsds/BetaineAnhydrous.pdfGoogle Scholar
EU–Safety Data Sheet (1999 c) Product Name: Betaine Monohydrate. http://www.aminoactives.com/pdf/eumsds/BetaineMonohydrate.pdfGoogle Scholar
Feng, J & Xu, ZR (2001) Effect of betaine on muscle, liver and serum amino acid composition in finishing swine. Journal of Zhejiang University Agriculture and Life Sciences 27, 107–110.Google Scholar
Feng, J & Yu, DY (2001) Effect of betaine on growth performance and methyl transfer function in finisher pigs. Chinese Journal of Animal Science 37, 8–10.Google Scholar
Ferket, PR (1995) Flushing syndrome in commercial turkeys during the grow-out stage. In Proceedings of the Smithkline Beecham Pacesetter Conference, National Turkey Federation Annual Meeting, 10 01 1995, pp. 5–14. Nutley, NJ: Smithkline Beecham Animal Health.Google Scholar
Fernandez, C, Lopez-Saez, A, Gallego, L & de la Fuente, JM (2000) Effect of source of betaine on growth performance and carcass traits in lambs. Animal Feed Science and Technology 86, 71–82.CrossRefGoogle Scholar
Fernandez, C, Gallego, L & Lopez-Bote, CJ (1998) Effect of betaine on fat content in growing lambs. Animal Feed Science and Technology 73, 329–338.CrossRefGoogle Scholar
Fernandez-Figares, I, Wray-Cahen, D, Steele, NC, Campbell, RG, Hall, DD, Virtanen, E & Caperna, TJ (2002) Effect of dietary betaine on nutrient utilization and partitioning in the young growing feed-restricted pig. Journal of Animal Science 80, 421–428.CrossRefGoogle Scholar
Finkelstein, JD (1998) The metabolism of homocysteine: pathways and regulation. European Journal of Pediatrics 157, Suppl. 2, S40–S44.CrossRefGoogle Scholar
Finkelstein, JD & Martin, JJ (1984) Methionine metabolism in mammals. Distribution of homocysteine between competing pathways. Journal of Biological Chemistry 259, 9508–9513.CrossRefGoogle ScholarPubMed
Firman, JD & Remus, JC (1999) Relationship between cystine and betaine in low methionine diets. Poultry Science 78, Suppl. 1, 135.Google Scholar
Flaim, KE, Li, JB & Jefferson, LS (1978) Protein turnover in rat skeletal muscle: effects of hypophysectomy and growth hormone. American Journal of Nutrition 234, E38–E43.Google ScholarPubMed
Florou-Paneri, P, Kufidis, DC, Vassilopoulos, VN & Spais, AV (1997) Performance of broiler chicks fed on low choline and methionine diets supplemented with betaine. Epitheorese Zootehnikes Epistemes 24, 103–111.Google Scholar
Garcia, MN, Chendrimada, TP, Pesti, GM & Bakalli, RI (1999) Relative bioavailability of two labile methyl sources methionine and betaine. Poultry Science 78, Suppl. 1, 135.Google Scholar
Garcia, MN, Pesti, GM & Bakalli, RI (2000) Influence of dietary protein level on the broiler chicken's response to methionine and betaine supplements. Poultry Science 79, 1478–1484.CrossRefGoogle Scholar
Goodman, AD, Hoekstra, S, Busch, RS, Meyer, GS & Abend, SS (1988) Effects of prolactin and growth hormone on tissue and serum carnitine in the rat. Endocrinology 123, 1955–1961.CrossRefGoogle ScholarPubMed
Graf, D, Kurz, AK, Reinehr, R, Fischer, R, Kircheis, G & Haussinger, D (2002) Prevention of bile acid–induced apoptosis by betaine in rat liver. Hepatology 36, 829–839.Google ScholarPubMed
Gralak, MA, Lesniewska, V, Puchala, R, Barej, W & Dymnicki, E (1998) The effect of betaine and rumen undegradable choline on growth rate and feed efficiency in calves. Journal of Animal and Feed Sciences 7, 229–233.CrossRefGoogle Scholar
Hafez, YS, Chavez, E, Vohra, P & Kratzer, FH (1978) Methionine toxicity in chicks and poults. Poultry Science 57, 699–703.CrossRefGoogle ScholarPubMed
Hanczakowska, E, Urbanczyk, J & Swiatkiewycz, M (1999) The efficiency of betaine and organic compounds of chromium in fattening of pigs with ad libitum or restricted feeding. Roczniki Naukowe Zootechniki 26, 263–274.Google Scholar
Haussinger, D (1998) Osmoregulation of liver cell function: signalling, osmolytes and cell heterogeneity. Contributions to Nephrology 123, 185–204.CrossRefGoogle ScholarPubMed
Haydon, KD, Campbell, RG & Prince, TJ (1995) Effect of, dietary betaine additions and amino:calorie ratio on performance and carcass traits of finishing pigs. Journal of Animal Science 73, Suppl. 1, 83.Google Scholar
Hochachka, PW & Somero, GN (1984) Biochemical Adaptation. Princeton, NJ: Princeton University Press.CrossRefGoogle Scholar
Kasai, K, Suzuki, H, Nakamura, T, Shiina, H & Shimoda, SI (1980) Glycine stimulated growth hormone release in man. Acta Endocrinologica (Copenhagen) 93, 283–286.Google ScholarPubMed
Kettunen, H, Peuranen, S, Apajalahti, J, Jatila, H, Nurminen, P & Saarinen, M (1999) Effect of betaine on the microbiology of the chicken gastrointestinal tract, p. 186. In Proceedings of the 12th European Symposium of Veldhofen, The Netherlands.Google Scholar
Kettunen, H, Peuranen, S & Tiihonen, K (2001 a) Betaine aids in the osmoregulation of duodenal epithelium of broiler chicks, and affects the movement of water across the small intestinal epithelium in vitro. Comparative Biochemistry and Physiology 129, 595–603.CrossRefGoogle ScholarPubMed
Kettunen, H, Peuranen, S, Tiihonen, K & Saarinen, M (2001 b) Intestinal uptake of betaine in vitro and the distribution of methyl groups from betaine, choline, and methionine in the body of broiler chicks. Comparative Biochemistry and Physiology 128, 269–278.CrossRefGoogle ScholarPubMed
Kettunen, H, Tiihonen, K, Peuranen, S, Saarinen, MT & Remus, JC (2001 c) Dietary betaine accumulates in the liver and intestinal tissue and stabilizes the intestinal epithelial structure in healthy and coccidia–infected broiler chicks. Comparative Biochemistry and Physiology 130, 759–769.CrossRefGoogle ScholarPubMed
Kidd, MT, Ferket, PR & Garlich, JD (1997) Nutritional and osmoregulatory functions of betaine. World's Poultry Science Journal 53, 125–139.CrossRefGoogle Scholar
Kitt, SJ, Miller, PS, Lewis, AJ & Chen, HY (1999) Effects of betaine and pen space allocation on growth performance, plasma urea concentration and carcass characteristics of growing and finishing barrows. Journal of Animal Science 77, Suppl. 1, 53.Google Scholar
Klasing, KC, Adler, KL, Remus, JC & Calvert, CC (2002) Dietary betaine increases intraepithelial lymphocytes in the duodenum of coccidian–infected chicks and increases functional properties of phagocytes. Journal of Nutrition 132, 2274–2282.CrossRefGoogle Scholar
Kuznetsov, VV & Shevyakova, NI (1997) Stress response of tobacco cells to high temperature and salinity. Proline accumulation and phosphorylation of polypeptides. Physiologia Plantarum 100, 320–326.CrossRefGoogle Scholar
Law, RO & Burg, MB (1991) The role of organic osmolytes in the regulation of mammalian cell volume. In Advances of Comparative and Environmental Physiology, vol. 9, Volume and Osmolality Control in Animal Cells, pp. 189–225. [Gilles, R, Hoffmann, EK and Bolis, L, editors]. New York: Springer Verlag.CrossRefGoogle Scholar
Lawrence, BV, Schinckel, AP, Adeola, O & Cera, K (2002) Impact of betaine on pig finishing performance and carcass composition. Journal of Animal Science 80, 475–482.CrossRefGoogle ScholarPubMed
LeMieux, FM, Southern, LL & Bidner, TD (1996) Interactive effects of chromium tripicolinate, zinc oxide and (or) betaine on growth performance of weanling pigs. Journal of Animal Science 74, Suppl. 1, 184.Google Scholar
Le Rudulier, D, Ström, AR, Dandekar, AM, Smith, LT & Valentine, RC (1984) Molecular biology of osmoregulation. Science 224, 1064–1068.CrossRefGoogle ScholarPubMed
Löest, CA, Drouillard, EC, Titgemeyer, EC, Hunter, RD & Wessel, RH (1998) Betaine as a dietary supplement for finishing cattle. In Cattlemen's Day, Report of Progress 804, pp. 76–78. Manhattan, KA: Agricultural Experimental Station, Kansas State University.Google Scholar
Löest, CA, Titgemeyer, EC, Drouillard, JS, Blasi, DA & Bindel, DJ (2001) Soybean hulls as a primary ingredient in forage-free diets for limit-fed growing cattle. Journal of Animal Science 79, 766–774.CrossRefGoogle ScholarPubMed
Löest, CA, Titgemeyer, EC & Greenwood, RH (1999) Role of methionine as a methyl group donor in cattle. In Cattlemen's Day, Report of Progress 831, pp. 114–116. Manhattan, KA: Agricultural Experimental Station, Kansas State University.Google Scholar
Lowry, KR, Izquierdo, QA & Baker, DH (1987) Efficacy of betaine relative to choline as a dietary methyl donor. Poultry Science 66, 135.Google Scholar
McDevitt, RM, Mack, S & Wallis, IR (2000) Can betaine partially replace or enhance the effect of methionine by improving broiler growth and carcase characteristics? British Poultry Science 41, 473–480.CrossRefGoogle ScholarPubMed
Matthews, JO, Southern, LL & Bidner, TD (2001 a) Estimation of the total sulfur amino acid requirement and the effect of betaine in diets deficient in total sulfur amino acids for the weanling pig. Journal of Animal Science 79, 1557–1565.CrossRefGoogle ScholarPubMed
Matthews, JO, Southern, LL, Bidner, TD & Persica, MA (2001 b) Effects of betaine, pen space, and slaughter handling method on growth performance, carcass traits, and pork quality of finishing barrows. Journal of Animal Science 79, 967–974.CrossRefGoogle ScholarPubMed
Matthews, JO, Southern, LL, Higbie, AD, Persica, MA & Bidner, TD (2001 c) Effects of betaine on growth, carcass characteristics, pork quality, and plasma metabolites in finishing pigs. Journal of Animal Science 79, 722–728.CrossRefGoogle ScholarPubMed
Matthews, JO, Southern, LL, Pontif, JE, Higbie, AD & Bidner, TD (1998) Interactive effects of betaine, crude protein, and net energy in finishing pigs. Journal of Animal Science 76, 2444–2455.CrossRefGoogle ScholarPubMed
Mitchell, AD, Chappell, A & Knox, KL (1979) Metabolism of betaine in the ruminant. Journal of Animal Science 49, 764–774.CrossRefGoogle ScholarPubMed
Moeckel, GW & Lien, YH (1997) Distribution of de novo synthetized betaine in rat kidney: role of renal synthesis of medullary betaine accumulation. American Journal of Physiology 272, F94–F99.Google Scholar
Moeckel, GW, Shadman, R, Fogel, JM & Sadrzadeh, SMH (2002) Organic osmolytes betaine, sorbitol and inositol are potent inhibitors of erythrocyte membrane ATPases. Life Sciences 71, 2413–2424.CrossRefGoogle ScholarPubMed
Mongin, P (1976) Ionic constituents and osmolality of the small intestinal fluids of the laying hen. British Poultry Science 17, 383–392.CrossRefGoogle ScholarPubMed
Nakanishi, TR, Turner, J & Burg, MB (1990) Osmoregulation of betaine transport in mammalian medullary cells. American Journal of Physiology 258, F1061–F1067.Google ScholarPubMed
National Research Council (1994) Nutrient Requirements of Poultry. 9th ed. Washington, DC: National Academy Press.Google Scholar
National Research Council (1998) Nutrient Requirements of Swine 10th ed. Washington, DC: National Academy Press.Google Scholar
Neill, AR, Grime, DW, Snoswell, AM, Northrop, AJ, Lindsay, DB & Dawson, RM (1979) The low availability of dietary choline for the nutrition of the sheep. Biochemical Journal 180, 559–565.CrossRefGoogle ScholarPubMed
Overland, M, Rorvik, KA & Skrede, A (1999) Effect of trimethylamine oxide and betaine in swine diets on growth performance, carcass characteristics, nutrient digestibility, and sensory quality of pork. Journal of Animal Science 77, 2143–2153.CrossRefGoogle ScholarPubMed
Owen, K, Nelssen, J, Goodband, R, Weeden, T & Blum, S (1996) Effect of L–carnitine and soyabean oil on growth performance and body composition of early weaned pigs. Journal of Animal Science 74, 1612–1619.CrossRefGoogle Scholar
Oyaas, K, Ellingsen, TE, Dyrset, N & Levine, DW (1995) Transport of osmoprotective compounds in hybridoma cells exposed to hyperosmotic stress. Cytotechnology 17, 143–151.CrossRefGoogle ScholarPubMed
Pesti, GM, Harper, AE & Sunde, ML (1979) Sulfur amino acid and methyl donor status of corn-soy diets for starting broiler chicks and turkey poults. Poultry Science 58, 1541–1547.CrossRefGoogle ScholarPubMed
Peters-Regehr, T, Bode, JG, Kubitz, R & Haussinger, D (1999) Organic osmolyte transport in quiescent and activated rat hepatic stellate cells (Ito cells). Hepatology 29, 173–180.CrossRefGoogle ScholarPubMed
Petronini, PG, Alfieri, RR, Losi, MN, Caccamo, AE, Cavazzoni, A, Bonelli, MA, Borghetti, AF & Wheeler, KP (2000) Induction of BGT–1 and amino acid system A transport activities in endothelial cells exposed to hyperosmolarity. American Journal of Physiology 279, R1580–R1589.Google ScholarPubMed
Petronini, PG, de Angelis, EM, Borghetti, P & Borghetti, AF (1992) Modulation by betaine of cellular response to osmotic stress. Journal of Biochemistry 282, 69–73.CrossRefGoogle Scholar
Petronini, PG, de Angelis, EM, Borghetti, AF & Wheeler, KP (1994) Osmolytically inducible uptake of betaine via amino acid transport system A in SV–3T3 cells. Biochemical Journal 300, 45–50.CrossRefGoogle ScholarPubMed
Pettigrew, JE & Esnaola, MA (2001) Swine nutrition and pork quality: a review. Journal of Animal Science 79, Suppl. E, E316–E342.CrossRefGoogle Scholar
Puchala, R, Zabielski, R, Lesniewska, P, Gralak, V, Kiela, P & Barej, W (1998) Influence of duodenal infusion of betaine or choline on blood metabolites and duodenal electrical activity in Friesian calves. Journal of Agricultural Science 131, 321–327.CrossRefGoogle Scholar
Puchala, RT, Sahlu, MJ, Herselman, JJ & Davis, SP (1995) Influence of betaine on blood metabolites of alpine and angora kids. Small Ruminant Research 18, 137–143.CrossRefGoogle Scholar
Remus, J, Virtanen, E, Rosi, L & McNaughton, J (1995) Effect of betaine on nutrient utilization of 21-day-old broilers during coccidiosis. In Proceedings of the 10th European Symposium on Poultry Nutrition, 15–19 10 1995, pp. 371–372. Antalya, Turkey: World Poultry Science Association.Google Scholar
Remus, JC & Quarles, CL (2000) The effect of betaine on lesion scores and tensile strength of coccidia-challenged broilers. Poultry Science 79, Suppl. 1, 118.Google Scholar
Robinson, SP & Jones, GP (1986) Accumulation of glycine betaine in chloroplasts provides osmotic adjustment during salt stress. Australian Journal of Plant Physiology 13, 659–668.Google Scholar
Rowling, MJ, McMullen, MH, Chipman, DC & Schalinske, KL (2002) Hepatic glycine N-methyltransferase is up-regulated by excess dietary methionine in rats. Journal of Nutrition 132, 2545–2550.CrossRefGoogle ScholarPubMed
Sakomura, NK, Kimura, ME, Junqueira, OM & Silva, R (1996) Utilization of betaine in broiler rations. Ars Veterinaria 12, 86–94.Google Scholar
Schrama, JW, Heetkamp, MJW, Simmins, PH & Gerrits, WJJ (2003) Dietary betaine supplementation affects energy metabolism of pigs. Journal of Animal Science 81, 1202–1209.CrossRefGoogle ScholarPubMed
Schutte, JB, de Jong, J, Smink, W & Pack, M (1997) Replacement value of betaine for DL–methionine in male broiler chicks. Poultry Science 76, 321–325.CrossRefGoogle ScholarPubMed
Schwab, U, Torronen, A, Toppinen, L, Alfthan, G, Saarinen, M, Aro, A & Uusitupa, M (2002) Betaine supplementation decreases plasma homocysteine concentrations but does not affect body weight, body composition or rest energy expenditure in human subjects. American Journal of Clinical Nutrition 76, 961–967.CrossRefGoogle Scholar
Shibata, T, Akamine, T, Nikki, T, Yamashita, H & Nobukini, K (2003) Synthesis of betaine–homocysteine S–methyl–transferase is continuously enhanced in fatty livers of thyroidectomized chickens. Poultry Science 82, 207–213.CrossRefGoogle Scholar
Sidransky, H & Farber, E (1960) Liver choline oxidase in man and in several species of animals. Archives of Biochemistry and Biophysics 87, 129–133.CrossRefGoogle ScholarPubMed
Siljander-Rasi, H, Peuranen, S, Tiihonen, K, Virtanen, E, Kettunen, H, Alaviuhkola, T & Simmins, PH (2003) Effect of equi-molar dietary betaine and choline addition on performance, carcass quality and physiological parameters of pigs. Animal Science 76, 55–62.CrossRefGoogle Scholar
Simon, J (1999) Choline, betaine and methionine interactions in chickens, pigs and fish (including crustaceans). World's Poultry Science Journal 55, 353–374.CrossRefGoogle Scholar
Smith, JW II, Nelssen, JL, Goodband, RD, Tokach, MD, Richert, BT, Owen, KQ, Bergstrom, JR & Blum, SA (1995) The effects of supplementing growing-finishing swine diets with betaine and (or) choline on growth and carcass characteristics. Journal of Animal Science 73, Suppl. 1, 83, Abstr.Google Scholar
Smith, JW II, Owen, KQ, Nelssen, JL, Goodband, RD, Tokach, MD, Friesen, KG, Lohrmann, TL & Blum, SA (1994) The effects of dietary carnitine, betaine, and chromium nicotinate supplementation on growth and carcass characteristics in growing-finishing pigs. Journal of Animal Science 72, Suppl. 1, 274.Google Scholar
Snoswell, AM & Xue, GP (1987) Methyl group metabolism in sheep. Comparative Biochemistry and Physiology 88, 383–394.Google ScholarPubMed
Souffrant, WB (1991) Endogenous nitrogen losses during digestion in pigs. In Proceedings of the 5th International Symposium on Digestive Physiology in Pigs, EAAP no. 54, pp. 147–166 [Versteegen, MWA, Huisman, J, den Hartog, LA, editors]. Wageningen, The Netherlands: EAAP.Google Scholar
Southern, LL, Brown, DR, Werner, DD & Fox, MC (1986) Excess of supplemental choline for swine. Journal of Animal Science 62, 992–996.CrossRefGoogle ScholarPubMed
Sowden, MP, Collins, HL, Smith, HC, Garrow, TA, Sparks, JD & Sparks, CE (1999) Apolipoprotein mRNA and lipoprotein secretion are increased in McArdle RH–7777 cells by expression of betaine–homocysteine S–methyltransferase. Biochemical Journal 341, 639–645.CrossRefGoogle ScholarPubMed
Sparks, JD & Sparks, CE (1994) Insulin regulation of triacylglycerol-rich lipoprotein synthesis and secretion. Biochimia et Biophysica Acta 1215, 9–23.CrossRefGoogle ScholarPubMed
Steinmetzer, W (1972) Contribution to the biochemistry and use of the beet constituent betaine. Zucker 25, 48–57.Google Scholar
Stekol, JA, Hsu, PT, Weiss, S & Smith, P (1953) Labile methyl group and its synthesis de novo in relation to growth of chicks. Biological Chemistry 203, 763–773.CrossRefGoogle ScholarPubMed
Stryer, L (1988) Biosynthesis of amino acids and heme. In Biochemistry, 3rd ed., pp. 575–626. New York: WH Freeman and Company.Google Scholar
Tan, AW & Ungar, F (1979) Growth hormone effects on creatine uptake by muscle in the hypophysectomized rat. Molecular Cellular Biochemistry 25, 67–77.CrossRefGoogle ScholarPubMed
Teeter, RG, Remus, JC, Belay, T, Mooney, M, Virtanen, E & Augustine, P (1999) The effects of betaine on water balance and performance in broilers reared under differing environmental conditions. In Proceedings of the Australian Poultry Science Symposium, p. 165 [Balnave, D, editor]. Sydney: University of Sydney Printing Service.Google Scholar
Tramacere, M, Petronini, PG, Severini, A & Borghetti, AF (1984) Osmoregulation of amino acid activity in cultured fibroblasts. Experimental Cell Research 151, 70–79.CrossRefGoogle ScholarPubMed
Tyler, DD (1977) Transport and oxidation of choline by liver mitochondria. Biochemical Journal 166, 571–581.CrossRefGoogle ScholarPubMed
Urbanczyk, J (1997) An attempt to decrease pig carcass fatness by nutritive factors. Krmiva 39, 311–325.Google Scholar
Urbanczyk, J, Hanczakowska, E & Swiatkiewycz, M (1999) Betaine and organic chromium as the feed additives in pig nutrition. Annals of Warsaw Agricultural University, Animal Science 36, 133–140.Google Scholar
Urbanczyk, J, Hanczakowska, E & Swiatkiewycz, M (2000) The efficiency of betaine and organic chromium compounds according to fattening pig genotype. Biuletyn Naukowy Przemyslu Paszowego 39, 53–64.Google Scholar
van Lunen, TA & Simmins, PH (2000) Dietary enzyme and betaine supplementation for young pigs. Canadian Journal of Animal Science 80, 755–756.Google Scholar
Virtanen, E, McNaughton, J, Rosi, L & Hall, D (1993) Effects of betaine supplementation on intestinal lesion, mortality and performance of coccidian–challenged broiler chicks. In Proceedings of the 9th European Symposium, on Poultry Nutrition, Jelenia, Göra, Poland, pp. 433–446. World's Poultry Science Association.Google Scholar
Virtanen, E & Rosi, L (1995) Effects of betaine on methionine requirement of broilers under various environmental conditions. In Proceedings of the Australian Poultry Science Symposium, pp. 88–92. Sydney, NSW, Australia: University of Sydney.Google Scholar
Wallis, IR (1999) Dietary supplements of methionine increase breast meat yield and decrease abdominal fat in growing broiler chickens. Australian Journal of Experimental Agriculture 39, 131–141.CrossRefGoogle Scholar
Wang, YZ (2000) Effect of betaine on growth performance and carcass traits of meat ducks. Journal of Zhejiang University Agricultural and Life Sciences 26, 347–352.Google Scholar
Wang, YZ & Xu, ZR (1999) Effect of feeding betaine on weight gain and carcass trait of barrows and gilts and approach to mechanism. Journal of Zhejiang Agricultural University 25, 281–285.Google Scholar
Wang, YZ, Xu, ZR, Chen, ML (2000 a) Effect of betaine on carcass fat metabolism of meat duck. Chinese Journal of Veterinary Science 20, 409–413.Google Scholar
Wang, YZ, Xu, ZR, Feng, J (2000 b) Study on the effect of betaine on meat quality and the mechanism in finishing pigs. Scientia Agricultura Sinica 33, 94–99.Google Scholar
Warren, LK, Lawrence, LM & Thompson, KN (1999) The influence of betaine on untrained and trained horses exercising to fatigue. Journal of Animal Science 77, 677–684.CrossRefGoogle ScholarPubMed
Warskulat, U, Wettstein, M, Häussinger, D (1995) Betaine is an osmolyte in RAW 264.7 mouse macrophages. FEBS Letters 377, 47–50.CrossRefGoogle ScholarPubMed
Webel, DM (1994) Effect of betaine supplementation on growth performance, carcass characteristics and nitrogen retention of finishing pigs. M.S. thesis, University of Illinois, Urbana.Google Scholar
Webel, DM, McKeith, FK & Easter, RA (1995) The effects of betaine supplementation on growth performance and carcass characteristics in finishing pigs. Journal of Animal Science 73, Suppl. 1, 82.Google Scholar
Weigand, E & Kirchgessner, W (1981) Betaine and glutamine acid contribution to nitrogen digestion and balance during feeding of vinasse to growing pigs. Archives of Animal Nutrition 31, 335–343.Google ScholarPubMed
Weik, C, Warskulat, U, Bode, J, Peters-Regehr, T, Häussinger, D (1998) Comparable organic osmolytes in rat liver endothelial cells. Hepatology 27, 787–793.Google Scholar
Westberg, JK (1951) Betaine in the Nutrition of Chickens and Turkeys. Chicago, IL: International Minerals and Chemical Corporation.Google Scholar
Wettstein, M, Weik, C, Holneicher, C, Häussinger, D (1998) Betaine as an osmolyte in rat liver: metabolism and cell–to–cell interactions. Hepatology 27, 787–793.CrossRefGoogle ScholarPubMed
Wiedmeier, RD, Tanner, BH, Bair, JR, Shenton, HT, Arambel, MJ & Walters, JL (1992) Effects of a new molasses by-product, concentrated separator by-product, on nutrient digestibility and ruminal fermentation in cattle. Journal of Animal Science 70, 1936–1940.CrossRefGoogle Scholar
Xing, WB & Rajashekar, CB (2001) Glycine betaine involvement in freezing tolerance and water stress in Arabidopsis thaliana. Environmental and Experimental Botany 46, 21–28.CrossRefGoogle ScholarPubMed
Xu, ZR & Feng, J (1998) Effect of betaine on carcass characteristics and approach to mechanism of the effect in finishing swine. Acta Veterinaria et Zootechnica Sinica 29, 397–405.Google Scholar
Xu, ZR, Wang, MQ, Huai, MY (1999 a) Approach of the mechanism of growth–promoting effect of betaine on swine. Chinese Journal of Veterinary Science 19, 399–403.Google Scholar
Xu, ZR & Yu, DY (2000) Effect of betaine on digestive function of weaned piglets. Chinese Journal of Veterinary Science 20, 201–204.Google Scholar
Xu, ZR, Yu, DY, Wang, YZ (1999 b) The effects of betaine on weanling piglets and its mechanism. Journal of Zhejiang Agricultural University 25, 543–546.Google Scholar
Xu, ZR, Yu, DY, Wang, YZ, Zhou, LX (1999 c) Effect of betaine on growth and digestive function of weaning piglets. Acta Agriculturae Zhejiangensis 11, 4.Google Scholar
Xu, ZR & Zhan, XA (1998) Effects of betaine on methionine and adipose metabolism in broiler chicks. Acta Veterinaria et Zootechnica Sinica 29, 212–219.Google Scholar
Xue, GP & Snoswell, AM (1985 a) Comparative studies on the methionine synthesis in sheep and rat tissues. Comparative Biochemistry and Physiology 80, 489–494.Google ScholarPubMed
Xue, GP & Snoswell, AM (1985 b) Regulation of methyl group metabolism in lactating ewes. Biochemistry International 11, 381–385.Google ScholarPubMed
Yalcin, S, Ergun, A & Colpan, I (1992) The effects of betaine supplementation on egg production and egg quality in laying hen. Veteriner Fakultesi, Dergisi, Universitesi Ankara 39, 325–335.Google Scholar
Yancey, PH & Burg, MB (1989) Distribution of major organic osmolytes in rabbit kidneys in diuresis and antidiuresis. American Journal of Physiology 257, F602–F607.Google ScholarPubMed
Yao, Z, McLeod, RS (1994) Synthesis and secretion of hepatic apolipoprotein B–containing lipoproteins. Biochimia et Biophysica Acta 1212, 152–166.CrossRefGoogle ScholarPubMed
Yao, Z & Vance, DE (1989) Reductions in VLDL but not HDL, in plasma of rats deficient in choline. Biochemistry and Cell Biology 68, 552–558.CrossRefGoogle Scholar
Yao, Z & Vance, DE (1990) Head group specificity in the requirement of phosphatidylcholine biosynthesis for very low density lipoprotein secretion from cultured hepatocytes. Biological Chemistry 264, 11373–11380.CrossRefGoogle Scholar
Yu, DY, Feng, J & Xu, ZR (2001) Effects of betaine on fat and protein metabolism in different stages of swine. Chinese Journal of Veterinary Science 21, 200–203.Google Scholar
Yu, DY & Xu, ZR (2000) Effects of methyl-donor on the performances and mechanisms of growth–promoting hormone in piglets. Chinese Journal of Animal Science 36, 8–10.Google Scholar
Zentek, J (2002) Egg taint – a problem of practical importance. Lohmann Information 28, 3–6.Google Scholar
Zhan, XA (2000) Studies on growth-promoting mechanism of betaine in broiler chickens. Acta Agriculturae Zhejiangensis 12, 209–212.Google Scholar
Zhan, XA & Xu, ZR (1999) Effects of betaine on meat quality and mechanism of the effects in finishing broilers. Journal of Zhejiang University Agriculture and Life Science 25, 611–614.Google Scholar
Zhang, F, Warskulat, U, Wettstein, M, Häussinger, D (1996) Identification of betaine as an osmolyte in rat liver macrophages (Kupffer cells). Gastroenterology 110, 1543–1552.CrossRefGoogle ScholarPubMed
Zou, XT (2001) Effects of betaine on endocrinology of laying hens and its mechanism of action. Chinese Journal of Veterinary Science 21, 300–303.Google Scholar
Zou, XT & Lu, JJ (2002) Effects of betaine on the regulation of the lipid metabolism in laying hen. Agricultural Sciences in China 1, 1043–1049.Google Scholar
Zou, XT, Ma, YL & Xu, ZR (1998) Effects of betaine and thyroprotein on laying performance and approach to mechanism of the effects in hens. Acta Agriculturae Zhejiangensis 10, 144–149.Google Scholar
Zou, XT, Xu, ZR & Wang, YZ (2002) Effects of methylamino acids on growth performance of swine in different stages. Journal of Zhejiang University Agriculture and Life Sciences 28, 551–555.Google Scholar