Bioactive compounds and their composition in coffee

Roasted coffee beans contain more than 1000 bioactive compounds with various physiological effects as well as health benefits^{(Reference Farah4,Reference Carneiro, Beatriz, Oliviera and Alves5)} . Active compounds and their derivatives contained in coffee have several neuroprotective properties that reduce the risk of cognitive decline and neurodegenerative diseases. A growing body of evidence suggests that regular consumption of coffee, as well as tea and dark chocolate (cocoa), may support a reduction in the risk of age-related neurodegenerative disorders. However, the complex array of phytochemicals in these components hinders a clear understanding of the components that influence neuronal plasticity and resilience^{(Reference Camandola, Plick and Mattson6)}. Anti-inflammatory (inhibition of the expression of cytokines genes, modulation of nuclear factor kappa-light chain enhancer of activated B cells, regeneration of neurons, neuroplasticity), antioxidant (elimination of free radicals, reduction of oxidative stress), antifibrotic (reduction of oxidative DNA damage, elimination of free radicals), antimicrobial (antimicrobial activity against a range of Gram-positive and Gram-negative bacteria) and anticancer (prevention of initiation of carcinogenesis, antitumoural activity) properties have also been described, leading to reduced mortality and improvements in endocrine, hepatic, gastrointestinal, cardiovascular and cancer diseases^{(Reference Wasim, Kukkar and Awad7–Reference Osarieme, Modupe and Oluchukwu10)}.

The optimal benefits obtained from coffee in pathologies depend on higher daily doses. Existing research believes that coffee has great therapeutic potential in the future, but there is still a need to elucidate the mechanisms of true causal relationships in certain neuropathologies in further studies.

Coffee is a complex blend of many compounds and nutrients that work synergistically together. However, its multidirectional effects and mechanism of action are still poorly understood. The composition of elements in coffee beans varies with the type of coffee, country of origin, growing conditions, roasting conditions, temperature, time, speed, brewing conditions and grind size of coffee beans^{(Reference Socała, Szopa and Serefko11)}. Caffeine (CAF) is one of the most important and discussed compounds not only in coffee but also in tea, cola and chocolate of different types and brands. The different abundances and contents of CAF are studied because of its many effects on the organism (genetic polymorphism, sex and metabolic heterogeneity)^{(Reference Hong, Chan and Bai12)}.

The most important compounds of coffee beans are:

1. 1) caffeine (0·5–2·6%): differences between Arabica (0·7–1·4%), Robusta (2·2–2·4%) and Excelsa (0·86–1·13%)

2. 2) theobromine, theophylline (1%)

3. 3) caffeic acid, quinic acid (10%)

4. 4) chlorogenic acid (4–6%)

5. 5) polysaccharides (25–30%)

6. 6) proteins (13%)

7. 7) fats and waxes (0·1–0·8%)

8. 8) water (10–13%)

9. 9) minerals (4%)

10. 10) vitamin B3 (trace amount)^{(Reference Wintgens13)}.

Among the most important physiologically effective compounds are:

1. 1) purine alkaloids – methylxanthines (CAF, theobromine, theophylline and nicotinic acid);

2. 2) polyphenols (chlorogenic acids (CGA) and their derivates such as CAF, quinic acids, phenylindanes, ferulic acid, quercetin, hydroxyhydroquinone and pyrocatechol);

3. 3) fatty acids (eicosanoyl-5-hydroxytryptamine);

4. 4) diterpenoids (cafestol and kahweol);

5. 5) flavonoids (anthocyanins and catechins);

6. 6) lactones;

7. 7) and many other micronutrients such as potassium and magnesium^{(Reference Wasim, Kukkar and Awad7,Reference Kolahdouzan and Hamadeh14,Reference Li, Hatamo and Hattori15)} .

Methylxanthines, of which CAF (1,3,7-trimethylxanthine) is best studied, have clear effects on neuronal network activity, protecting neurons from dysfunction and promoting sustained cognitive development. Since it is a purine alkaloid, it is described as an antioxidant, capable of protecting against oxidative damage, which is typical of most neurodegenerative diseases. Its mechanism of action is based on the antagonism of different subclasses of adenosine receptors, thereby mediating protection by dopamine receptors, stimulating the central nervous system and enhancing mood and alertness. In addition, it is a phosphodiesterase inhibitor, with a consequent effect on metabolism. It is most commonly associated with neuroprotective effects, mediated by the inhibition of lipid peroxidation and reduction of reactive oxygen species production^{(Reference Kolahdouzan and Hamadeh14)}. CAF has been shown to reduce excitotoxicity, cellular apoptosis and inflammation, as well as partially resolve memory problems. In Alzheimer’s disease (AD), CAF has been shown to protect against blood–brain barrier disruption, reducing amyloid beta (Aβ) levels through inhibition of its secretory pathway specifically, demonstrating its anti-inflammatory, antioxidant and mitochondrial activity, as well as neurological stimulation and neuroprotection. α-Tocopherol has demonstrated neuroprotective activity through antioxidant activity and free radical scavenging activity, which has been associated with inhibition of some forms of neurotoxicity and improvement of memory impairment in Parkinson’s disease (PD)^{(Reference Carneiro, Beatriz, Oliviera and Alves5)}.

Other representatives of methylxanthines are theobromine (3,7-dimethylxanthine) and theophylline (1,3-dimethylxanthine), also found in tea or chocolate. They show high bioavailability and are associated with hypoglycaemic and neuroprotective benefits. Moreover, theobromine levels inversely correlate with Aβ₄₂ levels in patients with AD^{(Reference Camandola, Plick and Mattson6)}.

CAF metabolites paraxanthine (1,7-dimethylxanthine) and theophylline (1,3-dimethylxanthine) provide neuroprotection against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) which induces dopamine loss in the striatum and shows inhibition of dopamine reduction^{(Reference Camandola, Plick and Mattson6)}. Whereas paraxanthine is not present in plant extracts, in humans its blood concentrations reach levels comparable or higher than those of CAF. Therefore, it should be considered when investigating the physiological effects of CAF, especially under conditions of chronic intake.

Mancini et al.^{(Reference Mancini, Wang and Weaver16)} investigated another group of coffee compounds – the phenylindanes. Using fluorescence assays to determine the level of inhibition of neurodegenerative protein accumulation, they found that the phenylindanes in dark-roasted coffee exhibited strong inhibitory activity, suggesting the importance of neuroprotection^{(Reference Mancini, Wang and Weaver16)}.

When coffee undergoes roasting, amino acids and carbohydrates react through a process called the Maillard reaction^{(Reference Martins, Jongen and Boekel17)}. Through this, other compounds such as melanoidins and acrylamide are produced. Moreira et al.^{(Reference Moreira, Nunes and Domingues18)} point to melanoidins as having high antioxidant activity, especially in scavenging free radicals. These bioactive substances were studied in rats that were given approximately six cups of espresso (1 espresso = 30 ml of caffeine source/60 mg caffeine content in the product) (to give an idea, Fig. 1 summarises the CAF content in caffeine-containing beverages). Melanoidins were able to decrease the concentration of pro-inflammatory cytokines (tumour necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ)) and increase the number of anti-inflammatory substances, such as interleukins (IL).

Figure 1. A summary of the ratio of the volume/quantity of the caffeine source to the amount of CAF contained in the product. The amount of CAF (mg) is recorded in individual coffee and other soft drinks, as well as in different types of chocolate. The size of the bubbles represents the amount of CAF in the product. Colour indicates product diversity: dark brown represents coffee drinks; light brown represents chocolate products; green represents tea drinks; and blue represents soft drinks. An interactive version is available at https://public.flourish.studio/visualisation/13411728/.

β-Carbolines harman and norharman are two compounds present in roasted coffee with a known spectrum of physiological effects in the human body. This is a group of chemicals widely found in nature and structurally very similar to the neurotoxin MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine), which has been linked to the development of PD. These compounds have recently been investigated for some neuroprotective effects. In particular, β-carbolines can reversibly inhibit monoamine oxidase (MAO) and increase the concentration of neurotransmitters such as dopamine, thus protecting against the neurodegenerative process. Indeed, MAO inhibition is increasingly being reported as an important mechanism for reducing the rate of PD, mainly by reducing the oxidative deamination of key neurotransmitters, including dopamine, the neurotransmitter most affected in this disease. While caffeine is reported to act as an antagonist for the adenosine A2 receptor, blocking the effect of adenosine on the suppression of dopaminergic transmission and, thus, reducing dopamine neurotoxicity, norharman and harman are potent competitive and reversible MAO inhibitors, both in rats and in humans. The high levels of these β-carbolines in roasted coffee place them at the top of the list of suspected compounds likely to influence the course of Parkinson’s disease^{(Reference Carneiro, Beatriz, Oliviera and Alves5,Reference Moreira, Nunes and Domingues18,Reference Casal19)} .

Chlorogenic acids (CGA) share not only antioxidant properties but also anti-inflammatory effects by inhibiting the expression of cytokine genes and modulating the activation of inflammatory nuclear factor kappa B (NFκB), which is associated with neuronal regeneration, thereby contributing to neuroplasticity. For this reason, together with CAF, they have attributes of neuroprotective properties^{(Reference Kitagawa, Houzen and Tashiro20)}.

Caffeine and Parkinson’s disease

Although there are CAF intake doses determined to be not to be associated with adverse effects by Health Canada (400 mg/d for adults (10 g for lethality), 300 mg/d for pregnant women and 2·5 mg/kg/d for children and adolescents), 2·5 mg CAF/kg body weight/d remains an appropriate recommendation^{(Reference Wikoff, Welsh and Henderson21)}. Because CAF is water and lipid soluble, it readily crosses the blood–brain barrier. It produces complex pharmacological effects in the brain, ranging from adenosine receptor antagonism to phosphodiesterase inhibition, and from to GABA receptor blockade and calcium release^{(Reference Ren and Chen22)}.

CAF levels and their metabolism can be analysed from blood or saliva. Deciphering the molecular networks distinguishing PD from healthy individuals and patients with other, unrelated PD diseases may lead to new insights into pathogenesis and identification of crucial biomarkers. Previous studies have confirmed the involvement of oxidative stress and dyshomoeostasis in catecholamine, tryptophan and CAF metabolism in PD^{(Reference Shao, Li and Liu23)}. In the moderate/ advanced PD group, patients with motor complications had significantly reduced basal salivary CAF concentrations, significantly longer disease duration and an increase in the Movement Disorder Society-sponsored revision of the Unified Parkinson’s Disease Rating Scale (MDS-UPDRS) compared with those without motor complications, whereas patients with early PD or de novo patients did not. There was a negative association with antiparkinsonian medication, supporting the hypothesis that CAF levels in PD are a marker of disease progression^{(Reference Leodori, De Bartolo and Belvisi24,Reference Gökcen and Şanlier25)} . Similarly, a protective effect of coffee has also been reported in dementia and AD, with CAF reversing cognitive impairment and reducing amyloid burden. The results of a study by Hong et al.^{(Reference Hong, Chan and Bai12)} suggest that coffee consumption among healthy individuals and patients with PD was significantly associated with a lower hazard ratio for the risk or progression of PD leading to a favourable causal relationship between CAF consumption and the risk of PD. A potential neuroprotective effect of CAF consumption has also been reported in epidemiological case–control studies. Another constituent in coffee, eicosanoyl-5-hydroxytryptamide, is thought to protect against neurodegeneration. A similar effect is also thought to occur with tea consumption. Instead of psychostimulation, CAF antagonises the adenosine A2A receptor. In the CNS, A2A is expressed exclusively in dopaminergic neurons, and activation of the adenosine A2A receptor triggers cAMP-proteinase A, which is dependent on an increase in intracellular calcium and release of glutamate. This suggests that excessive intracellular calcium and glutamate concentrations are responsible for excitotoxicity in neurodegeneration^{(Reference Hong, Chan and Bai12)}.

Mechanism of action of caffeine

Clinically, CAF appears to improve motor symptoms by non-selective antagonism at the adenosine A2A receptor^{(Reference Fujimaki, Saiki and Li26)}. A2A receptors are predominantly distributed in the putamen, nucleus caudatus, nucleus accumbens and globulus pallidus, while indirectly interacting with dopamine D2 receptors of the basal ganglia. In the study by Ishibashi et al.^{(Reference Ishibashi, Miura and Wagatsuma27)}, they report that CAF binds to striatal A2A receptors in a dose-dependent manner, with sufficiently occupied receptors obtainable by drinking at least one cup of coffee, equivalent to approximately 100 mg of CAF^{(Reference Ishibashi, Miura and Wagatsuma27)}.

Eicosanoyl-5-hydroxytryptamine (EHT) is a derivative of serotonin, whereas C₂₀ is a saturated eicosanoic fatty acid linked to the serotonin amino group. EHT purified from coffee serves as an agent leading to enhancement of the enzymatic activity of the specific phosphatase, protein phosphatase 2A (PPA2), which dephosphorylates pathogenic α-synuclein while working synergistically with CAF in PD and dementia with Lewy bodies (DLB). In the case of coffee, PPA2 thus inhibits the methylesterase and remains in a highly active methylated state. This synergy is through the enhancement of PPA2, which is dysregulated in the brains of patients with synucleinopathies. EHT is also an inhibitor of PME-1 methylesterase, thereby contributing to an increase in brain PPA2 methylation and phosphatase activity, which reduces the amount of phosphorylated α-synuclein (p-α-syn), improving neuronal integrity, suppressing neuroinflammation and preserving the behavioural phenotype. It also exhibits antioxidant and anti-inflammatory effects.

The catalytic subunit of a specific phosphatase, PP2A, which dephosphorylates α-synuclein, is hypomethylated in the brain of a patient with PD, preventing the assembly of an active trimeric holoenzyme and leading to a decrease in phosphatase activity. This phosphatase deficiency contributes to the accumulation of hyperphosphorylated α-synuclein, which tends to fibrillate more than unmodified α-synuclein.

CAF is primarily metabolised by cytochrome P450 (CYP), specifically the CYP1A2 and CYP2E1 subunits, protects against the dopaminergic neurotoxin MPTP and acts as an adenosine A2A receptor antagonist. However, in patients with early-onset PD, CAF consumption has no effect on the rate of disease progression nor have any associations between gene polymorphism and frequency of disease onset been confirmed^{(Reference Fujimaki, Saiki and Li26)}. Co-treatment of CAF with EHT reduces p-α-syn and protects against neuronal damage and neuroinflammation, as such treatment combination significantly reduces the number of p-α-syn immunoreactive cells in the brain and restores neuritic integrity. These results suggest that this treatment has a synergistic effect in protecting neuronal integrity and preventing inflammatory response^{(Reference Yan, Zhang and Park28)}.

A 4-year prospective study by Moccia et al.^{(Reference Moccia, Erro and Picillo29)} examined the clinical correlates of CAF consumption in a de novo patient population, in which they described that greater CAF consumption was associated with reduced deterioration in both motor and non-motor impairment. Concerning motor symptoms, higher CAF product use was associated with lower motor impairments throughout the study period, suggesting that CAF may have a symptomatic effect, possibly delaying an individual’s motor impairment. Pathophysiologically, the motor effect of CAF in PD is likely mediated by the inhibition of adenosine A2A receptors in striatopallidal neurons, which determines the improvement of parkinsonian symptoms. Consistent with this, there is another hypothesis that the motor function of patients with PD can be improved by a targeted strategy of incorporating CAF into the development of novel antiparkinsonian drugs. Since antiparkinsonian drugs can competitively affect CAF metabolism, significant improvement in patients with PD undergoing antiparkinsonian treatment has been observed^{(Reference Fujimaki, Saiki and Li26)}. Also, study results show that patients with PD with higher CAF use presented a reduced need for dopaminergic treatment^{(Reference Moccia, Erro and Picillo29)}.

Findings studied in mouse models suggest that subtherapeutic doses of CAF and EHT may synergistically act on biochemical and molecular changes in the mouse brain, leading to brain protection in synucleinopathies. This also preserves neuronal integrity and dampens the inflammatory response in phosphatase activity, which is associated with reduced p-α-syn accumulation. Similar biochemical changes are described in a cellular model in which there is a significant increase in PPA2 methylation and cytoprotection and a decrease in p-α-syn^{(Reference Yan, Zhang and Park28)}. In addition, antagonism of A2A receptors with selective inhibitors or genetic knock-out mimicked CAF protection in various experimental models of PD. In animal models of PD, caffeine treatment improved oxidative stress, restored striatal and midbrain dopamine depletion, prevented decline in motor activity and muscle strength, and improved norepinephrine levels^{(Reference Paul, Chuang and Shih30)}.

Caffeine change modelling tools

Liquid chromatography coupled with mass spectrometry (LC–MS), along with gas chromatography, is a powerful tool for profiling metabolite changes, using four CAF metabolites that have been confirmed as biomarkers of de novo PD progression^{(Reference Fujimaki, Saiki and Li26)}.