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Identification of four starch-branching enzymes in barley endosperm: partial purification of forms I, IIa and IIb

Published online by Cambridge University Press:  01 October 1997

CHUANXIN SUN ,
P. SATHISH ,
STAFFAN AHLANDSBERG ,
ANNA DEIBER  and
CHRISTER JANSSON
CHUANXIN SUN
Affiliation:
Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden
P. SATHISH
Affiliation:
Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden
STAFFAN AHLANDSBERG
Affiliation:
Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden
ANNA DEIBER
Affiliation:
Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden
CHRISTER JANSSON
Affiliation:
Department of Biochemistry, The Arrhenius Laboratories, Stockholm University, S-10691 Stockholm, Sweden
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Abstract

Developing caryopses of barley (Hordeum vulgare L. cv. Golf) were monitored for starch-branching enzyme activity for 1 month after anthesis. Homogenized caryopses from grains with the highest specific activity were used as starting material for purification of branching enzyme by FPLC chromatography. Several branching enzyme activity fractions were resolved. We have previously described the isolation of a 51/50 kDa protein with starch branching activity from one of these fractions. From three other fractions, we have now partially purified starch branching enzymes corresponding to forms I, IIa and IIb.

Keywords

Barley caryopsesstarch-branching enzymesstarch synthesis
Type
Research Article
Information
The New Phytologist , Volume 137 , Issue 2 , October 1997 , pp. 215 - 222
Copyright
© Trustees of the New Phytologist 1997

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