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RAPD analyses of recombination processes in the entomopathogenic fungus Beauveria bassiana

Published online by Cambridge University Press:  23 September 2003

Patricia R. DALZOTO ,
Chirlei GLIENKE-BLANCO ,
Vanessa KAVA-CORDEIRO ,
Welington Luiz ARAÚJO  and
João Lúcio AZEVEDO
Patricia R. DALZOTO
Affiliation:
Universidade Federal do Paraná, Departamento de Genética, C. Postal 19071, Curitiba PR, CEP 81531-990, Brazil. E-mail: [email protected]
Chirlei GLIENKE-BLANCO
Affiliation:
Universidade Federal do Paraná, Departamento de Genética, C. Postal 19071, Curitiba PR, CEP 81531-990, Brazil. E-mail: [email protected]
Vanessa KAVA-CORDEIRO
Affiliation:
Universidade Federal do Paraná, Departamento de Genética, C. Postal 19071, Curitiba PR, CEP 81531-990, Brazil. E-mail: [email protected]
Welington Luiz ARAÚJO
Affiliation:
Universidade de São Paulo, ESALQ, Piracicaba SP, Brazil.
João Lúcio AZEVEDO
Affiliation:
Universidade de São Paulo, ESALQ, Piracicaba SP, Brazil and Universidade de Mogi das Cruzes, Mogi das Cruzes SP, Brazil.
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Abstract

To understand the nature of recombination processes in Beauveria bassiana, double-auxotrophic complementary mutant strains were used to produce six heterokaryons by three different methods. Conidia from these heterokaryons were plated on selective media and stable haploid (but not diploid) recombinants were isolated. Single colony recombinants were recovered with both parental and non-parental random amplified polymorphic DNA (RAPD) profiles. These results suggest that a range of different recombination mechanisms may be occurring in B. bassiana.

Type
Research Article
Information
Mycological Research , Volume 107 , Issue 9 , September 2003 , pp. 1069 - 1074
Copyright
© The British Mycological Society 2003

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