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Identification and molecular phylogeny of Epulorhiza isolates from tropical orchids

Published online by Cambridge University Press:  23 September 2003

Ming MA ,
Teck Koon TAN  and
Sek Man WONG
Ming MA
Affiliation:
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Kent Ridge, 117543 Singapore. E-mail: [email protected] Present address: Institute of Molecular and Cell Biology, 1 Research Link, National University of Singapore, 117604 Singapore.
Teck Koon TAN
Affiliation:
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Kent Ridge, 117543 Singapore. E-mail: [email protected]
Sek Man WONG
Affiliation:
Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Kent Ridge, 117543 Singapore. E-mail: [email protected]
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Abstract

24 isolates of Epulorhiza were obtained from the roots and protocorms of orchids in Singapore. Two groups were distinguished based on morphological and cultural characteristics. Group 1 comprised 20 isolates which were identified as E. repens, while those in group 2 were identified as E. calendulina-like Rhizoctonia. The ITS-5.8S rDNA sequence identity ranged from 88–100% among isolates of E. repens and six sub-groups were further delineated. The sequence identity was 98–100% among E. calendulina-like Rhizoctonia isolates. The sequence identity between E. repens isolates and E. calendulina-like Rhizoctonia isolates ranged from 18–44%. Apparently, isolates from both groups 1 and 2 were genetically distinct. Phylogenetic analysis showed that the distribution of the isolates correlated with the sites and the hosts from which the fungi were isolated. No matching sequences have been found in the GenBank database for the ITS region of E. repens and E. calendulina-like Rhizoctonia.

Type
Research Article
Information
Mycological Research , Volume 107 , Issue 9 , September 2003 , pp. 1041 - 1049
Copyright
© The British Mycological Society 2003

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