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Heterologous diploid nuclei in the violet root rot fungus, Helicobasidium mompa

Published online by Cambridge University Press:  23 September 2003

Tadanori AIMI ,
Yoshinori IWASAKI ,
Sanae KANO ,
Yoshiki YOTSUTANI  and
Tsutomu MORINAGA
Tadanori AIMI
Affiliation:
Department of Bioresource Development, Hiroshima Prefectural University, Nanatsuka-cho 562, Shobara-shi, Hiroshima 727-0023, Japan. E-mail: [email protected]
Yoshinori IWASAKI
Affiliation:
Department of Bioresource Development, Hiroshima Prefectural University, Nanatsuka-cho 562, Shobara-shi, Hiroshima 727-0023, Japan. E-mail: [email protected]
Sanae KANO
Affiliation:
Department of Bioresource Development, Hiroshima Prefectural University, Nanatsuka-cho 562, Shobara-shi, Hiroshima 727-0023, Japan. E-mail: [email protected]
Yoshiki YOTSUTANI
Affiliation:
Department of Bioresource Development, Hiroshima Prefectural University, Nanatsuka-cho 562, Shobara-shi, Hiroshima 727-0023, Japan. E-mail: [email protected]
Tsutomu MORINAGA
Affiliation:
Department of Bioresource Development, Hiroshima Prefectural University, Nanatsuka-cho 562, Shobara-shi, Hiroshima 727-0023, Japan. E-mail: [email protected]
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Abstract

Allelic genes hga1-1 and hga1-2, which encode G protein alpha subunit in the violet root rot fungus, Helicobasidium mompa, were sequenced and characterized. Restriction fragment polymorphism (RFLP) analysis determined that the gene is present as a single locus in the single basidiospore isolates, while strain V169 possessed both alleles of this gene. Therefore, although basidiospore isolates are dikaryon, they are homokaryotic. Field-isolated strain V169, the putative parent strain, is a dikaryotic heterokaryon. Allelic genes hga1-1 and hga1-2 segregated in almost a 1[ratio ]3 ratio among single basidiospore isolates from the same fruiting body. Moreover, the copy number of hga1-1 was found to be less than that of hga1-2 in the V169 strain. These results suggest that one of the nuclei in the V169 parent strain is homozygous diploid and the other heterozygous diploid. This parent strain produced four homokaryotic and dikaryon basidiospores on each basidium.

Type
Research Article
Information
Mycological Research , Volume 107 , Issue 9 , September 2003 , pp. 1060 - 1068
Copyright
© The British Mycological Society 2003

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