Species-specific PCR primers in the rDNA-ITS region as a diagnostic tool for Serpula lacrymans

O. SCHMIDT; U. MORETH

doi:10.1017/S0953756299001562

Abstract

The ITS of the nuclear ribosomal DNA of Serpula lacrymans and S. himantioides was amplified by PCR to evaluate this DNA region as a diagnostic tool. Sequencing of the amplified ITS region revealed a size of the complete ITS of 654 bp for S. lacrymans and 650 bp for S. himantioides. The base sequence difference between the two fungi was used to design species-specific oligonucleotide PCR primers for both species. With laboratory and field samples, each primer selectively detected its target organism. No cross reaction occurred with other fungi causing rot of indoor wood.

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Species-specific PCR primers in the rDNA-ITS region as a diagnostic tool for Serpula lacrymans

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Species-specific PCR primers in the rDNA-ITS region as a diagnostic tool for Serpula lacrymans

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