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Thiol coupling based synthesis of temperature-sensitive polymer–peptide conjugates with controlled architecture.

Published online by Cambridge University Press:  18 May 2012

Jean-Baptiste Guilbaud ,
Aline F. Miller  and
Alberto Saiani
Jean-Baptiste Guilbaud
Affiliation:
School of Materials, The University of Manchester, Manchester, M1 7HS, UK
Aline F. Miller
Affiliation:
CEAS, The University of Manchester, Manchester, M60 1QD, UK
Alberto Saiani
Affiliation:
School of Materials, The University of Manchester, Manchester, M1 7HS, UK
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Abstract

A synthetic strategy to couple selectively an ionic complementary thiol modified octapeptide, that is able to gel at low temperature, to the thermoresponsive polymer poly(N-isopropylacrylamide) (pNIPAAm) with controlled molecular weight and narrow polydispersity is described. The polymer was synthesized by atom transfer radical polymerization (ATRP) affording halogen functionalized chain ends. This allowed subsequent coupling to a thiol terminated ionic complementary octapeptide via nucleophile substitution. Results indicated that the peptide was covalently attached to the polymer and that both the coil-globule phase transition of pNIPAAm and the gelation properties of the peptide were retained in the conjugated product. This method provides a versatile route for the synthesis of a range of bioconjugate materials with controlled architecture and dual self-assembling and thermoresponsive behavior.

Keywords

polymerbiomaterialpolymerization
Type
Articles
Information
MRS Online Proceedings Library (OPL) , Volume 1418: Symposium LL/MM – Gels and Biomedical Materials , 2012 , mrsf11-1418-ll09-06
Copyright
Copyright © Materials Research Society 2012

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References

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